Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.17/4121 |
Resumo: | The FEEDMI Study (NCT03663556) evaluated the influence of infant feeding (mother's own milk (MOM), donor human milk (DHM) and formula) on the fecal microbiota composition and alkaline phosphatase (ALP) activity in extremely and very preterm infants (≤32 gestational weeks). In this observational study, preterm infants were recruited within the first 24 h after birth. Meconium and fecal samples were collected at four time points (between the 2nd and the 26th postnatal days. Fecal microbiota was analyzed by RT-PCR and by 16S rRNA sequencing. Fecal ALP activity, a proposed specific biomarker of necrotizing enterocolitis (NEC), was evaluated by spectrophotometry at the 26th postnatal day. A total of 389 fecal samples were analyzed from 117 very preterm neonates. Human milk was positively associated with beneficial bacteria, such as Bifidobacterium, Bacteroides ovatus, and Akkermancia muciniphila, as well as bacterial richness. Neonates fed with human milk during the first week of life had increased Bifidobacterium content and fecal ALP activity on the 26th postnatal day. These findings point out the importance of MOM and DHM in the establishment of fecal microbiota on neonates prematurely delivered. Moreover, these results suggest an ALP pathway by which human milk may protect against NEC. |
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Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase ActivityMAC PEDMAC DIEHumansFemaleMaleAlkaline Phosphatase / metabolism*Feces / microbiologyGastrointestinal Microbiome / physiology*Gestational AgeInfant Formula / microbiologyInfant Nutritional Physiological Phenomena / physiology*Infant, NewbornInfant, Extremely Premature / physiology*Longitudinal StudiesMilk, Human / microbiology*RNA, Ribosomal, 16S / analysisThe FEEDMI Study (NCT03663556) evaluated the influence of infant feeding (mother's own milk (MOM), donor human milk (DHM) and formula) on the fecal microbiota composition and alkaline phosphatase (ALP) activity in extremely and very preterm infants (≤32 gestational weeks). In this observational study, preterm infants were recruited within the first 24 h after birth. Meconium and fecal samples were collected at four time points (between the 2nd and the 26th postnatal days. Fecal microbiota was analyzed by RT-PCR and by 16S rRNA sequencing. Fecal ALP activity, a proposed specific biomarker of necrotizing enterocolitis (NEC), was evaluated by spectrophotometry at the 26th postnatal day. A total of 389 fecal samples were analyzed from 117 very preterm neonates. Human milk was positively associated with beneficial bacteria, such as Bifidobacterium, Bacteroides ovatus, and Akkermancia muciniphila, as well as bacterial richness. Neonates fed with human milk during the first week of life had increased Bifidobacterium content and fecal ALP activity on the 26th postnatal day. These findings point out the importance of MOM and DHM in the establishment of fecal microbiota on neonates prematurely delivered. Moreover, these results suggest an ALP pathway by which human milk may protect against NEC.MDPIRepositório do Centro Hospitalar Universitário de Lisboa Central, EPEMorais, JMarques, CFaria, ATeixeira, DBarreiros-Mota, IDurão, CAraújo, JIsmael, SBrito, SCardoso, MMacedo, IPereira, ETomé, TCalhau, C2022-06-15T15:05:39Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.17/4121engNutrients. 2021 May 6;13(5):1564.10.3390/nu13051564.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-10T09:45:25Zoai:repositorio.chlc.min-saude.pt:10400.17/4121Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:21:27.808640Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity |
title |
Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity |
spellingShingle |
Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity Morais, J MAC PED MAC DIE Humans Female Male Alkaline Phosphatase / metabolism* Feces / microbiology Gastrointestinal Microbiome / physiology* Gestational Age Infant Formula / microbiology Infant Nutritional Physiological Phenomena / physiology* Infant, Newborn Infant, Extremely Premature / physiology* Longitudinal Studies Milk, Human / microbiology* RNA, Ribosomal, 16S / analysis |
title_short |
Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity |
title_full |
Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity |
title_fullStr |
Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity |
title_full_unstemmed |
Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity |
title_sort |
Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity |
author |
Morais, J |
author_facet |
Morais, J Marques, C Faria, A Teixeira, D Barreiros-Mota, I Durão, C Araújo, J Ismael, S Brito, S Cardoso, M Macedo, I Pereira, E Tomé, T Calhau, C |
author_role |
author |
author2 |
Marques, C Faria, A Teixeira, D Barreiros-Mota, I Durão, C Araújo, J Ismael, S Brito, S Cardoso, M Macedo, I Pereira, E Tomé, T Calhau, C |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório do Centro Hospitalar Universitário de Lisboa Central, EPE |
dc.contributor.author.fl_str_mv |
Morais, J Marques, C Faria, A Teixeira, D Barreiros-Mota, I Durão, C Araújo, J Ismael, S Brito, S Cardoso, M Macedo, I Pereira, E Tomé, T Calhau, C |
dc.subject.por.fl_str_mv |
MAC PED MAC DIE Humans Female Male Alkaline Phosphatase / metabolism* Feces / microbiology Gastrointestinal Microbiome / physiology* Gestational Age Infant Formula / microbiology Infant Nutritional Physiological Phenomena / physiology* Infant, Newborn Infant, Extremely Premature / physiology* Longitudinal Studies Milk, Human / microbiology* RNA, Ribosomal, 16S / analysis |
topic |
MAC PED MAC DIE Humans Female Male Alkaline Phosphatase / metabolism* Feces / microbiology Gastrointestinal Microbiome / physiology* Gestational Age Infant Formula / microbiology Infant Nutritional Physiological Phenomena / physiology* Infant, Newborn Infant, Extremely Premature / physiology* Longitudinal Studies Milk, Human / microbiology* RNA, Ribosomal, 16S / analysis |
description |
The FEEDMI Study (NCT03663556) evaluated the influence of infant feeding (mother's own milk (MOM), donor human milk (DHM) and formula) on the fecal microbiota composition and alkaline phosphatase (ALP) activity in extremely and very preterm infants (≤32 gestational weeks). In this observational study, preterm infants were recruited within the first 24 h after birth. Meconium and fecal samples were collected at four time points (between the 2nd and the 26th postnatal days. Fecal microbiota was analyzed by RT-PCR and by 16S rRNA sequencing. Fecal ALP activity, a proposed specific biomarker of necrotizing enterocolitis (NEC), was evaluated by spectrophotometry at the 26th postnatal day. A total of 389 fecal samples were analyzed from 117 very preterm neonates. Human milk was positively associated with beneficial bacteria, such as Bifidobacterium, Bacteroides ovatus, and Akkermancia muciniphila, as well as bacterial richness. Neonates fed with human milk during the first week of life had increased Bifidobacterium content and fecal ALP activity on the 26th postnatal day. These findings point out the importance of MOM and DHM in the establishment of fecal microbiota on neonates prematurely delivered. Moreover, these results suggest an ALP pathway by which human milk may protect against NEC. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z 2022-06-15T15:05:39Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.17/4121 |
url |
http://hdl.handle.net/10400.17/4121 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nutrients. 2021 May 6;13(5):1564. 10.3390/nu13051564. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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