Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity

Detalhes bibliográficos
Autor(a) principal: Morais, J
Data de Publicação: 2021
Outros Autores: Marques, C, Faria, A, Teixeira, D, Barreiros-Mota, I, Durão, C, Araújo, J, Ismael, S, Brito, S, Cardoso, M, Macedo, I, Pereira, E, Tomé, T, Calhau, C
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.17/4121
Resumo: The FEEDMI Study (NCT03663556) evaluated the influence of infant feeding (mother's own milk (MOM), donor human milk (DHM) and formula) on the fecal microbiota composition and alkaline phosphatase (ALP) activity in extremely and very preterm infants (≤32 gestational weeks). In this observational study, preterm infants were recruited within the first 24 h after birth. Meconium and fecal samples were collected at four time points (between the 2nd and the 26th postnatal days. Fecal microbiota was analyzed by RT-PCR and by 16S rRNA sequencing. Fecal ALP activity, a proposed specific biomarker of necrotizing enterocolitis (NEC), was evaluated by spectrophotometry at the 26th postnatal day. A total of 389 fecal samples were analyzed from 117 very preterm neonates. Human milk was positively associated with beneficial bacteria, such as Bifidobacterium, Bacteroides ovatus, and Akkermancia muciniphila, as well as bacterial richness. Neonates fed with human milk during the first week of life had increased Bifidobacterium content and fecal ALP activity on the 26th postnatal day. These findings point out the importance of MOM and DHM in the establishment of fecal microbiota on neonates prematurely delivered. Moreover, these results suggest an ALP pathway by which human milk may protect against NEC.
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spelling Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase ActivityMAC PEDMAC DIEHumansFemaleMaleAlkaline Phosphatase / metabolism*Feces / microbiologyGastrointestinal Microbiome / physiology*Gestational AgeInfant Formula / microbiologyInfant Nutritional Physiological Phenomena / physiology*Infant, NewbornInfant, Extremely Premature / physiology*Longitudinal StudiesMilk, Human / microbiology*RNA, Ribosomal, 16S / analysisThe FEEDMI Study (NCT03663556) evaluated the influence of infant feeding (mother's own milk (MOM), donor human milk (DHM) and formula) on the fecal microbiota composition and alkaline phosphatase (ALP) activity in extremely and very preterm infants (≤32 gestational weeks). In this observational study, preterm infants were recruited within the first 24 h after birth. Meconium and fecal samples were collected at four time points (between the 2nd and the 26th postnatal days. Fecal microbiota was analyzed by RT-PCR and by 16S rRNA sequencing. Fecal ALP activity, a proposed specific biomarker of necrotizing enterocolitis (NEC), was evaluated by spectrophotometry at the 26th postnatal day. A total of 389 fecal samples were analyzed from 117 very preterm neonates. Human milk was positively associated with beneficial bacteria, such as Bifidobacterium, Bacteroides ovatus, and Akkermancia muciniphila, as well as bacterial richness. Neonates fed with human milk during the first week of life had increased Bifidobacterium content and fecal ALP activity on the 26th postnatal day. These findings point out the importance of MOM and DHM in the establishment of fecal microbiota on neonates prematurely delivered. Moreover, these results suggest an ALP pathway by which human milk may protect against NEC.MDPIRepositório do Centro Hospitalar Universitário de Lisboa Central, EPEMorais, JMarques, CFaria, ATeixeira, DBarreiros-Mota, IDurão, CAraújo, JIsmael, SBrito, SCardoso, MMacedo, IPereira, ETomé, TCalhau, C2022-06-15T15:05:39Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.17/4121engNutrients. 2021 May 6;13(5):1564.10.3390/nu13051564.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-10T09:45:25Zoai:repositorio.chlc.min-saude.pt:10400.17/4121Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:21:27.808640Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity
title Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity
spellingShingle Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity
Morais, J
MAC PED
MAC DIE
Humans
Female
Male
Alkaline Phosphatase / metabolism*
Feces / microbiology
Gastrointestinal Microbiome / physiology*
Gestational Age
Infant Formula / microbiology
Infant Nutritional Physiological Phenomena / physiology*
Infant, Newborn
Infant, Extremely Premature / physiology*
Longitudinal Studies
Milk, Human / microbiology*
RNA, Ribosomal, 16S / analysis
title_short Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity
title_full Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity
title_fullStr Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity
title_full_unstemmed Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity
title_sort Influence of Human Milk on Very Preterms' Gut Microbiota and Alkaline Phosphatase Activity
author Morais, J
author_facet Morais, J
Marques, C
Faria, A
Teixeira, D
Barreiros-Mota, I
Durão, C
Araújo, J
Ismael, S
Brito, S
Cardoso, M
Macedo, I
Pereira, E
Tomé, T
Calhau, C
author_role author
author2 Marques, C
Faria, A
Teixeira, D
Barreiros-Mota, I
Durão, C
Araújo, J
Ismael, S
Brito, S
Cardoso, M
Macedo, I
Pereira, E
Tomé, T
Calhau, C
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório do Centro Hospitalar Universitário de Lisboa Central, EPE
dc.contributor.author.fl_str_mv Morais, J
Marques, C
Faria, A
Teixeira, D
Barreiros-Mota, I
Durão, C
Araújo, J
Ismael, S
Brito, S
Cardoso, M
Macedo, I
Pereira, E
Tomé, T
Calhau, C
dc.subject.por.fl_str_mv MAC PED
MAC DIE
Humans
Female
Male
Alkaline Phosphatase / metabolism*
Feces / microbiology
Gastrointestinal Microbiome / physiology*
Gestational Age
Infant Formula / microbiology
Infant Nutritional Physiological Phenomena / physiology*
Infant, Newborn
Infant, Extremely Premature / physiology*
Longitudinal Studies
Milk, Human / microbiology*
RNA, Ribosomal, 16S / analysis
topic MAC PED
MAC DIE
Humans
Female
Male
Alkaline Phosphatase / metabolism*
Feces / microbiology
Gastrointestinal Microbiome / physiology*
Gestational Age
Infant Formula / microbiology
Infant Nutritional Physiological Phenomena / physiology*
Infant, Newborn
Infant, Extremely Premature / physiology*
Longitudinal Studies
Milk, Human / microbiology*
RNA, Ribosomal, 16S / analysis
description The FEEDMI Study (NCT03663556) evaluated the influence of infant feeding (mother's own milk (MOM), donor human milk (DHM) and formula) on the fecal microbiota composition and alkaline phosphatase (ALP) activity in extremely and very preterm infants (≤32 gestational weeks). In this observational study, preterm infants were recruited within the first 24 h after birth. Meconium and fecal samples were collected at four time points (between the 2nd and the 26th postnatal days. Fecal microbiota was analyzed by RT-PCR and by 16S rRNA sequencing. Fecal ALP activity, a proposed specific biomarker of necrotizing enterocolitis (NEC), was evaluated by spectrophotometry at the 26th postnatal day. A total of 389 fecal samples were analyzed from 117 very preterm neonates. Human milk was positively associated with beneficial bacteria, such as Bifidobacterium, Bacteroides ovatus, and Akkermancia muciniphila, as well as bacterial richness. Neonates fed with human milk during the first week of life had increased Bifidobacterium content and fecal ALP activity on the 26th postnatal day. These findings point out the importance of MOM and DHM in the establishment of fecal microbiota on neonates prematurely delivered. Moreover, these results suggest an ALP pathway by which human milk may protect against NEC.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2022-06-15T15:05:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.17/4121
url http://hdl.handle.net/10400.17/4121
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nutrients. 2021 May 6;13(5):1564.
10.3390/nu13051564.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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