Biofilm formation by ST17 and ST19 strains of Streptococcus agalactiae

Detalhes bibliográficos
Autor(a) principal: Silvestre, Inês
Data de Publicação: 2020
Outros Autores: Borrego, Maria José, Jordão, Luísa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/7363
Resumo: Bacterial biofilms are an important virulence factor with a vital role in evasion from the host immune system, colonization and infection. The aim of the present study was to evaluate in vitro the effects of three environmental factors (H+, glucose and human plasma) in biofilm formation, by carrier and invasive Streptococcus agalactiae strains of ST17 and ST19 sequence types, including DNase producers and non-producers. Bacteria ability to assemble biofilms was classified based on crystal violet assay. Biofilm formation was also monitored by scanning electron microscopy. Depending on the growth medium used, each bacterial isolate could fit in different biofilm production categories. Our data showed that optimal conditions for S. agalactiae biofilm assembly were reached after 48 h incubation at pH 7.6 in the presence of glucose and inactivated human plasma. In the presence of inactivated human plasma, the biofilm biomass of ST19 strains experienced a higher increase than ST17 strains. The composition of the extracellular polymeric matrix of the three strongest biofilm producers (all from ST17) was accessed by enzymatic digestion of mature biofilms and proteins were shown to be the predominant component. The detailed identification of the extracellular protein components should contribute to the development of new therapeutic strategies to fight S. agalactiae infections.
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spelling Biofilm formation by ST17 and ST19 strains of Streptococcus agalactiaeStreptococcus agalactiaeBiofilmsExtracellular Polymeric Substance MatrixDNasesBacterial biofilms are an important virulence factor with a vital role in evasion from the host immune system, colonization and infection. The aim of the present study was to evaluate in vitro the effects of three environmental factors (H+, glucose and human plasma) in biofilm formation, by carrier and invasive Streptococcus agalactiae strains of ST17 and ST19 sequence types, including DNase producers and non-producers. Bacteria ability to assemble biofilms was classified based on crystal violet assay. Biofilm formation was also monitored by scanning electron microscopy. Depending on the growth medium used, each bacterial isolate could fit in different biofilm production categories. Our data showed that optimal conditions for S. agalactiae biofilm assembly were reached after 48 h incubation at pH 7.6 in the presence of glucose and inactivated human plasma. In the presence of inactivated human plasma, the biofilm biomass of ST19 strains experienced a higher increase than ST17 strains. The composition of the extracellular polymeric matrix of the three strongest biofilm producers (all from ST17) was accessed by enzymatic digestion of mature biofilms and proteins were shown to be the predominant component. The detailed identification of the extracellular protein components should contribute to the development of new therapeutic strategies to fight S. agalactiae infections.Elsevier/ Institut PasteurRepositório Científico do Instituto Nacional de SaúdeSilvestre, InêsBorrego, Maria JoséJordão, Luísa2021-03-08T15:53:50Z2020-122020-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7363engRes Microbiol. 2020 Dec;171(8):311-318. doi: 10.1016/j.resmic.2020.08.001. Epub 2020 Sep 5.0923-250810.1016/j.resmic.2020.08.001info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:58Zoai:repositorio.insa.pt:10400.18/7363Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:00.627625Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Biofilm formation by ST17 and ST19 strains of Streptococcus agalactiae
title Biofilm formation by ST17 and ST19 strains of Streptococcus agalactiae
spellingShingle Biofilm formation by ST17 and ST19 strains of Streptococcus agalactiae
Silvestre, Inês
Streptococcus agalactiae
Biofilms
Extracellular Polymeric Substance Matrix
DNases
title_short Biofilm formation by ST17 and ST19 strains of Streptococcus agalactiae
title_full Biofilm formation by ST17 and ST19 strains of Streptococcus agalactiae
title_fullStr Biofilm formation by ST17 and ST19 strains of Streptococcus agalactiae
title_full_unstemmed Biofilm formation by ST17 and ST19 strains of Streptococcus agalactiae
title_sort Biofilm formation by ST17 and ST19 strains of Streptococcus agalactiae
author Silvestre, Inês
author_facet Silvestre, Inês
Borrego, Maria José
Jordão, Luísa
author_role author
author2 Borrego, Maria José
Jordão, Luísa
author2_role author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Silvestre, Inês
Borrego, Maria José
Jordão, Luísa
dc.subject.por.fl_str_mv Streptococcus agalactiae
Biofilms
Extracellular Polymeric Substance Matrix
DNases
topic Streptococcus agalactiae
Biofilms
Extracellular Polymeric Substance Matrix
DNases
description Bacterial biofilms are an important virulence factor with a vital role in evasion from the host immune system, colonization and infection. The aim of the present study was to evaluate in vitro the effects of three environmental factors (H+, glucose and human plasma) in biofilm formation, by carrier and invasive Streptococcus agalactiae strains of ST17 and ST19 sequence types, including DNase producers and non-producers. Bacteria ability to assemble biofilms was classified based on crystal violet assay. Biofilm formation was also monitored by scanning electron microscopy. Depending on the growth medium used, each bacterial isolate could fit in different biofilm production categories. Our data showed that optimal conditions for S. agalactiae biofilm assembly were reached after 48 h incubation at pH 7.6 in the presence of glucose and inactivated human plasma. In the presence of inactivated human plasma, the biofilm biomass of ST19 strains experienced a higher increase than ST17 strains. The composition of the extracellular polymeric matrix of the three strongest biofilm producers (all from ST17) was accessed by enzymatic digestion of mature biofilms and proteins were shown to be the predominant component. The detailed identification of the extracellular protein components should contribute to the development of new therapeutic strategies to fight S. agalactiae infections.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
2020-12-01T00:00:00Z
2021-03-08T15:53:50Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/7363
url http://hdl.handle.net/10400.18/7363
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Res Microbiol. 2020 Dec;171(8):311-318. doi: 10.1016/j.resmic.2020.08.001. Epub 2020 Sep 5.
0923-2508
10.1016/j.resmic.2020.08.001
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dc.publisher.none.fl_str_mv Elsevier/ Institut Pasteur
publisher.none.fl_str_mv Elsevier/ Institut Pasteur
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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