Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Model

Detalhes bibliográficos
Autor(a) principal: Silva, Joana
Data de Publicação: 2019
Outros Autores: Alves, Celso, Freitas, Rafaela, Martins, Alice, Pinteus, Susete, Ribeiro, Joana, Gaspar, Helena, Alfonso, Amparo, Pedrosa, Rui
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/5071
Resumo: This work was supported by the Portuguese Foundation for Science and Technology (FCT) through Strategic Projects UID/MAR/04292/2013 and UID/Multi/04046/2019 granted to MARE—Marine and Environmental Sciences Centre, and BioISI—BioSystems and Integrative Sciences Institute, respectively, through Red2Discovery Project (PTDC/MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145-FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015 -LISBOA-01-0145-FEDER-016405) and through CrossAtlantic Project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI-01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund.
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spelling Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Model6-hydroxydopamineSeaweedsSH-SY5Y cellsNeuroprotectionMitochondrial membrane potentialCaspase-3 activityEleganoloneEleganonalNeurodegenerative diseasesThis work was supported by the Portuguese Foundation for Science and Technology (FCT) through Strategic Projects UID/MAR/04292/2013 and UID/Multi/04046/2019 granted to MARE—Marine and Environmental Sciences Centre, and BioISI—BioSystems and Integrative Sciences Institute, respectively, through Red2Discovery Project (PTDC/MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145-FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015 -LISBOA-01-0145-FEDER-016405) and through CrossAtlantic Project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI-01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund.Authors are very grateful for the financial support of the Projects and Programmes described in funding section. FCT is also acknowledged for the grants attributed to JS (SFRH/BD/103255/2014), CA (SFRH/BD/97764/2013), and SP (SFRH/BD/96203/2013).Bifurcaria bifurcata is a marine brown seaweed mainly found on the Atlantic coast. Herein, we report the antioxidant and neuroprotective activities of seven fractions (F1–F7) obtained by normal phase chromatography from the B. bifurcata dichloromethane extract, as well as of its two major isolated diterpenes. Total phenolic content of fractions was determined by the Folin–Ciocalteu method, while antioxidant activity was evaluated by the DPPH, ORAC, and FRAP assays. Neuroprotective effects were evaluated in a neurotoxic model induced by 6-hydroxydopamine (6-OHDA) in a human neuroblastoma cell line (SH-SY5Y), while the mechanisms associated to neuroprotection were investigated by the determination of mitochondrial membrane potential, H2O2 production, Caspase-3 activity, and by observation of DNA fragmentation. Fractions F4 and F5 exhibited the best neuroprotective and antioxidant activities, respectively. F4 fraction prevented changes in mitochondrial potential, and induced a reduction of H2O2 levels production and an increase in cell viability, suggesting that it may contain multi-target compounds acting on different pathways. Hence, this fraction was subjected to purification steps, affording the known diterpenes eleganolone and eleganonal. Both compounds exhibited antioxidant potential, being interesting candidates for further neuroprotective studies.MDPIIC-OnlineSilva, JoanaAlves, CelsoFreitas, RafaelaMartins, AlicePinteus, SuseteRibeiro, JoanaGaspar, HelenaAlfonso, AmparoPedrosa, Rui2020-08-12T10:51:45Z20192019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/5071engSilva, J., Alves, C., Freitas, R., Martins, A., Pinteus, S., Ribeiro, J., Gaspar, H., Alfonso, A., & Pedrosa, R. (2019). Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson's Disease Model. Marine drugs, 17(2), 85. https://doi.org/10.3390/md170200851660-339710.3390/md17020085info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:50:34Zoai:iconline.ipleiria.pt:10400.8/5071Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:48:45.522910Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Model
title Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Model
spellingShingle Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Model
Silva, Joana
6-hydroxydopamine
Seaweeds
SH-SY5Y cells
Neuroprotection
Mitochondrial membrane potential
Caspase-3 activity
Eleganolone
Eleganonal
Neurodegenerative diseases
title_short Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Model
title_full Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Model
title_fullStr Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Model
title_full_unstemmed Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Model
title_sort Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson’s Disease Model
author Silva, Joana
author_facet Silva, Joana
Alves, Celso
Freitas, Rafaela
Martins, Alice
Pinteus, Susete
Ribeiro, Joana
Gaspar, Helena
Alfonso, Amparo
Pedrosa, Rui
author_role author
author2 Alves, Celso
Freitas, Rafaela
Martins, Alice
Pinteus, Susete
Ribeiro, Joana
Gaspar, Helena
Alfonso, Amparo
Pedrosa, Rui
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Silva, Joana
Alves, Celso
Freitas, Rafaela
Martins, Alice
Pinteus, Susete
Ribeiro, Joana
Gaspar, Helena
Alfonso, Amparo
Pedrosa, Rui
dc.subject.por.fl_str_mv 6-hydroxydopamine
Seaweeds
SH-SY5Y cells
Neuroprotection
Mitochondrial membrane potential
Caspase-3 activity
Eleganolone
Eleganonal
Neurodegenerative diseases
topic 6-hydroxydopamine
Seaweeds
SH-SY5Y cells
Neuroprotection
Mitochondrial membrane potential
Caspase-3 activity
Eleganolone
Eleganonal
Neurodegenerative diseases
description This work was supported by the Portuguese Foundation for Science and Technology (FCT) through Strategic Projects UID/MAR/04292/2013 and UID/Multi/04046/2019 granted to MARE—Marine and Environmental Sciences Centre, and BioISI—BioSystems and Integrative Sciences Institute, respectively, through Red2Discovery Project (PTDC/MAR-BIO/6149/2014), co-financed by COMPETE (POCI-01-0145-FEDER-016791), through Oncologia de Precisão: Terapias e Tecnologias Inovadoras project (POINT4PAC) (SAICTPAC/0019/2015 -LISBOA-01-0145-FEDER-016405) and through CrossAtlantic Project (PTDC/BIA-OUT/29250/2017), co-financed by COMPETE (POCI-01-0145-FEDER-029250). This work was also funded by the Integrated Programme of SR&TD Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate (reference Centro-01-0145-FEDER-000018), co-funded by Centro 2020 Programme, Portugal 2020, European Union, through the European Regional Development Fund.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-01-01T00:00:00Z
2020-08-12T10:51:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/5071
url http://hdl.handle.net/10400.8/5071
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Silva, J., Alves, C., Freitas, R., Martins, A., Pinteus, S., Ribeiro, J., Gaspar, H., Alfonso, A., & Pedrosa, R. (2019). Antioxidant and neuroprotective potential of the brown seaweed Bifurcaria bifurcata in an in vitro Parkinson's Disease Model. Marine drugs, 17(2), 85. https://doi.org/10.3390/md17020085
1660-3397
10.3390/md17020085
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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