The blp locus of Streptococcus pneumoniae plays a limited role in the selection of strains that can cocolonize the human nasopharynx
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1128/AEM.01048-16 |
Resumo: | Nasopharyngeal colonization is important for Streptococcus pneumoniae evolution, providing the opportunity for horizontal gene transfer when multiple strains co-occur. Although colonization with more than one strain of pneumococcus is common, the factors that influence the ability of strains to coexist are not known. A highly variable blp (bacteriocin-like peptide) locus has been identified in all sequenced strains of S. pneumoniae. This locus controls the regulation and secretion of bacteriocins, small peptides that target other bacteria. In this study, we analyzed a series of cocolonizing isolates to evaluate the impact of the blp locus on human colonization to determine whether competitive phenotypes of bacteriocin secretion restrict cocolonization. We identified a collection of 135 nasopharyngeal samples cocolonized with two or more strains, totaling 285 isolates. The blp locus of all strains was characterized genetically with regard to pheromone type, bacteriocin/immunity content, and potential for locus functionality. Inhibitory phenotypes of bacteriocin secretion and locus activity were assessed through overlay assays. Isolates from single colonizations (n=298) were characterized for comparison. Cocolonizing strains had a high diversity of blp cassettes; approximately one-third displayed an inhibitory phenotype in vitro. Despite in vitro evidence of competition, pneumococci cocolonized the subjects independently of blp pheromone type (P=0.577), bacteriocin/immunity content, blp locus activity (P=0.798), and inhibitory phenotype (P=0.716). In addition, no significant differences were observed when single and cocolonizing strains were compared. Despite clear evidence of blp-mediated competition in experimental models, the results of our study suggest that the blp locus plays a limited role in restricting pneumococcal cocolonization in humans. |
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The blp locus of Streptococcus pneumoniae plays a limited role in the selection of strains that can cocolonize the human nasopharynxBiotechnologyFood ScienceApplied Microbiology and BiotechnologyEcologyNasopharyngeal colonization is important for Streptococcus pneumoniae evolution, providing the opportunity for horizontal gene transfer when multiple strains co-occur. Although colonization with more than one strain of pneumococcus is common, the factors that influence the ability of strains to coexist are not known. A highly variable blp (bacteriocin-like peptide) locus has been identified in all sequenced strains of S. pneumoniae. This locus controls the regulation and secretion of bacteriocins, small peptides that target other bacteria. In this study, we analyzed a series of cocolonizing isolates to evaluate the impact of the blp locus on human colonization to determine whether competitive phenotypes of bacteriocin secretion restrict cocolonization. We identified a collection of 135 nasopharyngeal samples cocolonized with two or more strains, totaling 285 isolates. The blp locus of all strains was characterized genetically with regard to pheromone type, bacteriocin/immunity content, and potential for locus functionality. Inhibitory phenotypes of bacteriocin secretion and locus activity were assessed through overlay assays. Isolates from single colonizations (n=298) were characterized for comparison. Cocolonizing strains had a high diversity of blp cassettes; approximately one-third displayed an inhibitory phenotype in vitro. Despite in vitro evidence of competition, pneumococci cocolonized the subjects independently of blp pheromone type (P=0.577), bacteriocin/immunity content, blp locus activity (P=0.798), and inhibitory phenotype (P=0.716). In addition, no significant differences were observed when single and cocolonizing strains were compared. Despite clear evidence of blp-mediated competition in experimental models, the results of our study suggest that the blp locus plays a limited role in restricting pneumococcal cocolonization in humans.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)Instituto de Higiene e Medicina Tropical (IHMT)Population health, policies and services (PPS)Global Health and Tropical Medicine (GHTM)RUNValente, CarinaDawid, SuzannePinto, Francisco R.Hinds, JasonSimões, Alexandra S.Gould, Katherine A.Mendes, Luís A.de Lencastre, HermíniaSá-Leão, Raquel2018-05-11T22:06:33Z20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10application/pdfhttps://doi.org/10.1128/AEM.01048-16eng0099-2240PURE: 2455969http://www.scopus.com/inward/record.url?scp=84987912459&partnerID=8YFLogxKhttps://doi.org/10.1128/AEM.01048-16info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:20:10Zoai:run.unl.pt:10362/36645Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:36.721157Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The blp locus of Streptococcus pneumoniae plays a limited role in the selection of strains that can cocolonize the human nasopharynx |
title |
The blp locus of Streptococcus pneumoniae plays a limited role in the selection of strains that can cocolonize the human nasopharynx |
spellingShingle |
The blp locus of Streptococcus pneumoniae plays a limited role in the selection of strains that can cocolonize the human nasopharynx Valente, Carina Biotechnology Food Science Applied Microbiology and Biotechnology Ecology |
title_short |
The blp locus of Streptococcus pneumoniae plays a limited role in the selection of strains that can cocolonize the human nasopharynx |
title_full |
The blp locus of Streptococcus pneumoniae plays a limited role in the selection of strains that can cocolonize the human nasopharynx |
title_fullStr |
The blp locus of Streptococcus pneumoniae plays a limited role in the selection of strains that can cocolonize the human nasopharynx |
title_full_unstemmed |
The blp locus of Streptococcus pneumoniae plays a limited role in the selection of strains that can cocolonize the human nasopharynx |
title_sort |
The blp locus of Streptococcus pneumoniae plays a limited role in the selection of strains that can cocolonize the human nasopharynx |
author |
Valente, Carina |
author_facet |
Valente, Carina Dawid, Suzanne Pinto, Francisco R. Hinds, Jason Simões, Alexandra S. Gould, Katherine A. Mendes, Luís A. de Lencastre, Hermínia Sá-Leão, Raquel |
author_role |
author |
author2 |
Dawid, Suzanne Pinto, Francisco R. Hinds, Jason Simões, Alexandra S. Gould, Katherine A. Mendes, Luís A. de Lencastre, Hermínia Sá-Leão, Raquel |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Tecnologia Química e Biológica António Xavier (ITQB) Instituto de Higiene e Medicina Tropical (IHMT) Population health, policies and services (PPS) Global Health and Tropical Medicine (GHTM) RUN |
dc.contributor.author.fl_str_mv |
Valente, Carina Dawid, Suzanne Pinto, Francisco R. Hinds, Jason Simões, Alexandra S. Gould, Katherine A. Mendes, Luís A. de Lencastre, Hermínia Sá-Leão, Raquel |
dc.subject.por.fl_str_mv |
Biotechnology Food Science Applied Microbiology and Biotechnology Ecology |
topic |
Biotechnology Food Science Applied Microbiology and Biotechnology Ecology |
description |
Nasopharyngeal colonization is important for Streptococcus pneumoniae evolution, providing the opportunity for horizontal gene transfer when multiple strains co-occur. Although colonization with more than one strain of pneumococcus is common, the factors that influence the ability of strains to coexist are not known. A highly variable blp (bacteriocin-like peptide) locus has been identified in all sequenced strains of S. pneumoniae. This locus controls the regulation and secretion of bacteriocins, small peptides that target other bacteria. In this study, we analyzed a series of cocolonizing isolates to evaluate the impact of the blp locus on human colonization to determine whether competitive phenotypes of bacteriocin secretion restrict cocolonization. We identified a collection of 135 nasopharyngeal samples cocolonized with two or more strains, totaling 285 isolates. The blp locus of all strains was characterized genetically with regard to pheromone type, bacteriocin/immunity content, and potential for locus functionality. Inhibitory phenotypes of bacteriocin secretion and locus activity were assessed through overlay assays. Isolates from single colonizations (n=298) were characterized for comparison. Cocolonizing strains had a high diversity of blp cassettes; approximately one-third displayed an inhibitory phenotype in vitro. Despite in vitro evidence of competition, pneumococci cocolonized the subjects independently of blp pheromone type (P=0.577), bacteriocin/immunity content, blp locus activity (P=0.798), and inhibitory phenotype (P=0.716). In addition, no significant differences were observed when single and cocolonizing strains were compared. Despite clear evidence of blp-mediated competition in experimental models, the results of our study suggest that the blp locus plays a limited role in restricting pneumococcal cocolonization in humans. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z 2018-05-11T22:06:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1128/AEM.01048-16 |
url |
https://doi.org/10.1128/AEM.01048-16 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0099-2240 PURE: 2455969 http://www.scopus.com/inward/record.url?scp=84987912459&partnerID=8YFLogxK https://doi.org/10.1128/AEM.01048-16 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
10 application/pdf |
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