Dual on–off and off–on switchable oligoaziridine biosensor
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4643 |
Resumo: | A water-soluble biocompatible aziridine-based biosensor with pendant anthracene units was synthesized by radicalar polymerization of N-substituted aziridines in supercritical carbon dioxide. The binding ability of the sensor towards a series of metal ions was examined by comparing the fluorescence intensities of the solutions before and after the addition of 100 equivalents of a solution of the metal ion chloride salt. A fast, simple and highly optical sensitive dual behavior, “off–on” and “on–off” response, was observed after the biosensor was exposed to the metal cations in aqueous solution. Zinc presented the highest fluorescence enhancement (turn-on) and copper presented the highest fluorescence quenching (turn-off). The response time was found to be instantaneous and the detection limit was achieved even in the presence of excess metal cation competitors. By using immunofluorescence microscopy it was also shown that oligoaziridine acts as an “on–off” probe through highly sensitive (detection limit of 1.6 nM), selective and reversible binding to copper anions under physiologic conditions using living Human Fibroblast cells. The stoichiometry for the reaction of the biosensor with Cu2+ was determined by a Job plot and indicates the formation of an oligoaziridine-Cu2+ 1:2 adduct. |
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Dual on–off and off–on switchable oligoaziridine biosensorOligoaziridinesBiosensorsSupercritical carbon dioxideCopperFluorescenceA water-soluble biocompatible aziridine-based biosensor with pendant anthracene units was synthesized by radicalar polymerization of N-substituted aziridines in supercritical carbon dioxide. The binding ability of the sensor towards a series of metal ions was examined by comparing the fluorescence intensities of the solutions before and after the addition of 100 equivalents of a solution of the metal ion chloride salt. A fast, simple and highly optical sensitive dual behavior, “off–on” and “on–off” response, was observed after the biosensor was exposed to the metal cations in aqueous solution. Zinc presented the highest fluorescence enhancement (turn-on) and copper presented the highest fluorescence quenching (turn-off). The response time was found to be instantaneous and the detection limit was achieved even in the presence of excess metal cation competitors. By using immunofluorescence microscopy it was also shown that oligoaziridine acts as an “on–off” probe through highly sensitive (detection limit of 1.6 nM), selective and reversible binding to copper anions under physiologic conditions using living Human Fibroblast cells. The stoichiometry for the reaction of the biosensor with Cu2+ was determined by a Job plot and indicates the formation of an oligoaziridine-Cu2+ 1:2 adduct.ElsevieruBibliorumRaje, VivekMorgado, Patrícia I.Ribeiro, MP.Correia, Ilídio Joaquim SobreiraBonifácio, VascoBranco, Paula S.Ricardo, Ana Aguiar2018-03-20T09:34:15Z2013-01-152013-01-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4643engRaje, V.P., Morgado, P.I., Ribeiro, M.P., Correia, I.J., Bonifácio, V.D.B., Branco, P.S. e Aguiar-Ricardo, A. (2013) “Dual On-Off and Off-On Switchable Oligoaziridine Biosensor”, Biosensors and Bioelectronics, Vol. 39(1), pp. 64–6910.1016/j.bios.2012.06.047metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-27T12:14:59Zoai:ubibliorum.ubi.pt:10400.6/4643Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-27T12:14:59Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Dual on–off and off–on switchable oligoaziridine biosensor |
title |
Dual on–off and off–on switchable oligoaziridine biosensor |
spellingShingle |
Dual on–off and off–on switchable oligoaziridine biosensor Raje, Vivek Oligoaziridines Biosensors Supercritical carbon dioxide Copper Fluorescence |
title_short |
Dual on–off and off–on switchable oligoaziridine biosensor |
title_full |
Dual on–off and off–on switchable oligoaziridine biosensor |
title_fullStr |
Dual on–off and off–on switchable oligoaziridine biosensor |
title_full_unstemmed |
Dual on–off and off–on switchable oligoaziridine biosensor |
title_sort |
Dual on–off and off–on switchable oligoaziridine biosensor |
author |
Raje, Vivek |
author_facet |
Raje, Vivek Morgado, Patrícia I. Ribeiro, MP. Correia, Ilídio Joaquim Sobreira Bonifácio, Vasco Branco, Paula S. Ricardo, Ana Aguiar |
author_role |
author |
author2 |
Morgado, Patrícia I. Ribeiro, MP. Correia, Ilídio Joaquim Sobreira Bonifácio, Vasco Branco, Paula S. Ricardo, Ana Aguiar |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Raje, Vivek Morgado, Patrícia I. Ribeiro, MP. Correia, Ilídio Joaquim Sobreira Bonifácio, Vasco Branco, Paula S. Ricardo, Ana Aguiar |
dc.subject.por.fl_str_mv |
Oligoaziridines Biosensors Supercritical carbon dioxide Copper Fluorescence |
topic |
Oligoaziridines Biosensors Supercritical carbon dioxide Copper Fluorescence |
description |
A water-soluble biocompatible aziridine-based biosensor with pendant anthracene units was synthesized by radicalar polymerization of N-substituted aziridines in supercritical carbon dioxide. The binding ability of the sensor towards a series of metal ions was examined by comparing the fluorescence intensities of the solutions before and after the addition of 100 equivalents of a solution of the metal ion chloride salt. A fast, simple and highly optical sensitive dual behavior, “off–on” and “on–off” response, was observed after the biosensor was exposed to the metal cations in aqueous solution. Zinc presented the highest fluorescence enhancement (turn-on) and copper presented the highest fluorescence quenching (turn-off). The response time was found to be instantaneous and the detection limit was achieved even in the presence of excess metal cation competitors. By using immunofluorescence microscopy it was also shown that oligoaziridine acts as an “on–off” probe through highly sensitive (detection limit of 1.6 nM), selective and reversible binding to copper anions under physiologic conditions using living Human Fibroblast cells. The stoichiometry for the reaction of the biosensor with Cu2+ was determined by a Job plot and indicates the formation of an oligoaziridine-Cu2+ 1:2 adduct. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-15 2013-01-15T00:00:00Z 2018-03-20T09:34:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4643 |
url |
http://hdl.handle.net/10400.6/4643 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Raje, V.P., Morgado, P.I., Ribeiro, M.P., Correia, I.J., Bonifácio, V.D.B., Branco, P.S. e Aguiar-Ricardo, A. (2013) “Dual On-Off and Off-On Switchable Oligoaziridine Biosensor”, Biosensors and Bioelectronics, Vol. 39(1), pp. 64–69 10.1016/j.bios.2012.06.047 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817549604238917632 |