Dual on–off and off–on switchable oligoaziridine biosensor

Detalhes bibliográficos
Autor(a) principal: Raje, Vivek
Data de Publicação: 2013
Outros Autores: Morgado, Patrícia I., Ribeiro, MP., Correia, Ilídio Joaquim Sobreira, Bonifácio, Vasco, Branco, Paula S., Ricardo, Ana Aguiar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.6/4643
Resumo: A water-soluble biocompatible aziridine-based biosensor with pendant anthracene units was synthesized by radicalar polymerization of N-substituted aziridines in supercritical carbon dioxide. The binding ability of the sensor towards a series of metal ions was examined by comparing the fluorescence intensities of the solutions before and after the addition of 100 equivalents of a solution of the metal ion chloride salt. A fast, simple and highly optical sensitive dual behavior, “off–on” and “on–off” response, was observed after the biosensor was exposed to the metal cations in aqueous solution. Zinc presented the highest fluorescence enhancement (turn-on) and copper presented the highest fluorescence quenching (turn-off). The response time was found to be instantaneous and the detection limit was achieved even in the presence of excess metal cation competitors. By using immunofluorescence microscopy it was also shown that oligoaziridine acts as an “on–off” probe through highly sensitive (detection limit of 1.6 nM), selective and reversible binding to copper anions under physiologic conditions using living Human Fibroblast cells. The stoichiometry for the reaction of the biosensor with Cu2+ was determined by a Job plot and indicates the formation of an oligoaziridine-Cu2+ 1:2 adduct.
id RCAP_2249f64efb3d7b15aafb2be9315f390d
oai_identifier_str oai:ubibliorum.ubi.pt:10400.6/4643
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Dual on–off and off–on switchable oligoaziridine biosensorOligoaziridinesBiosensorsSupercritical carbon dioxideCopperFluorescenceA water-soluble biocompatible aziridine-based biosensor with pendant anthracene units was synthesized by radicalar polymerization of N-substituted aziridines in supercritical carbon dioxide. The binding ability of the sensor towards a series of metal ions was examined by comparing the fluorescence intensities of the solutions before and after the addition of 100 equivalents of a solution of the metal ion chloride salt. A fast, simple and highly optical sensitive dual behavior, “off–on” and “on–off” response, was observed after the biosensor was exposed to the metal cations in aqueous solution. Zinc presented the highest fluorescence enhancement (turn-on) and copper presented the highest fluorescence quenching (turn-off). The response time was found to be instantaneous and the detection limit was achieved even in the presence of excess metal cation competitors. By using immunofluorescence microscopy it was also shown that oligoaziridine acts as an “on–off” probe through highly sensitive (detection limit of 1.6 nM), selective and reversible binding to copper anions under physiologic conditions using living Human Fibroblast cells. The stoichiometry for the reaction of the biosensor with Cu2+ was determined by a Job plot and indicates the formation of an oligoaziridine-Cu2+ 1:2 adduct.ElsevieruBibliorumRaje, VivekMorgado, Patrícia I.Ribeiro, MP.Correia, Ilídio Joaquim SobreiraBonifácio, VascoBranco, Paula S.Ricardo, Ana Aguiar2018-03-20T09:34:15Z2013-01-152013-01-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4643engRaje, V.P., Morgado, P.I., Ribeiro, M.P., Correia, I.J., Bonifácio, V.D.B., Branco, P.S. e Aguiar-Ricardo, A. (2013) “Dual On-Off and Off-On Switchable Oligoaziridine Biosensor”, Biosensors and Bioelectronics, Vol. 39(1), pp. 64–6910.1016/j.bios.2012.06.047metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-27T12:14:59Zoai:ubibliorum.ubi.pt:10400.6/4643Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-27T12:14:59Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Dual on–off and off–on switchable oligoaziridine biosensor
title Dual on–off and off–on switchable oligoaziridine biosensor
spellingShingle Dual on–off and off–on switchable oligoaziridine biosensor
Raje, Vivek
Oligoaziridines
Biosensors
Supercritical carbon dioxide
Copper
Fluorescence
title_short Dual on–off and off–on switchable oligoaziridine biosensor
title_full Dual on–off and off–on switchable oligoaziridine biosensor
title_fullStr Dual on–off and off–on switchable oligoaziridine biosensor
title_full_unstemmed Dual on–off and off–on switchable oligoaziridine biosensor
title_sort Dual on–off and off–on switchable oligoaziridine biosensor
author Raje, Vivek
author_facet Raje, Vivek
Morgado, Patrícia I.
Ribeiro, MP.
Correia, Ilídio Joaquim Sobreira
Bonifácio, Vasco
Branco, Paula S.
Ricardo, Ana Aguiar
author_role author
author2 Morgado, Patrícia I.
Ribeiro, MP.
Correia, Ilídio Joaquim Sobreira
Bonifácio, Vasco
Branco, Paula S.
Ricardo, Ana Aguiar
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv uBibliorum
dc.contributor.author.fl_str_mv Raje, Vivek
Morgado, Patrícia I.
Ribeiro, MP.
Correia, Ilídio Joaquim Sobreira
Bonifácio, Vasco
Branco, Paula S.
Ricardo, Ana Aguiar
dc.subject.por.fl_str_mv Oligoaziridines
Biosensors
Supercritical carbon dioxide
Copper
Fluorescence
topic Oligoaziridines
Biosensors
Supercritical carbon dioxide
Copper
Fluorescence
description A water-soluble biocompatible aziridine-based biosensor with pendant anthracene units was synthesized by radicalar polymerization of N-substituted aziridines in supercritical carbon dioxide. The binding ability of the sensor towards a series of metal ions was examined by comparing the fluorescence intensities of the solutions before and after the addition of 100 equivalents of a solution of the metal ion chloride salt. A fast, simple and highly optical sensitive dual behavior, “off–on” and “on–off” response, was observed after the biosensor was exposed to the metal cations in aqueous solution. Zinc presented the highest fluorescence enhancement (turn-on) and copper presented the highest fluorescence quenching (turn-off). The response time was found to be instantaneous and the detection limit was achieved even in the presence of excess metal cation competitors. By using immunofluorescence microscopy it was also shown that oligoaziridine acts as an “on–off” probe through highly sensitive (detection limit of 1.6 nM), selective and reversible binding to copper anions under physiologic conditions using living Human Fibroblast cells. The stoichiometry for the reaction of the biosensor with Cu2+ was determined by a Job plot and indicates the formation of an oligoaziridine-Cu2+ 1:2 adduct.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-15
2013-01-15T00:00:00Z
2018-03-20T09:34:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.6/4643
url http://hdl.handle.net/10400.6/4643
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Raje, V.P., Morgado, P.I., Ribeiro, M.P., Correia, I.J., Bonifácio, V.D.B., Branco, P.S. e Aguiar-Ricardo, A. (2013) “Dual On-Off and Off-On Switchable Oligoaziridine Biosensor”, Biosensors and Bioelectronics, Vol. 39(1), pp. 64–69
10.1016/j.bios.2012.06.047
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817549604238917632