The effect of dietary fatty acid on breast cancer lipidome
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/13294 |
Resumo: | Breast cancer is a complex heterogeneous disease and one of the leading causes of death among women. Many studies have tried to determine if saturated, monounsaturated and polyunsaturated fatty acids (FA) have different effects on the development of breast cancer. However, the role of different dietary FAs in lipidome of breast cancer cells is not completely understood. The aim of this work was to establish if lipidome of breast cancer T47-D cell line differs with different dietary FAs and correlate this findings to effects in proliferation and apoptosis. The lipid changes in lipidome induced by the presence of 1 μg/mL oleic acid (OA1) or 1 or 2 μg/mL linoleic (LA1 or LA2, respectively) acid in vitro were study through a lipidomic analysis, based on techniques such as TLC and GC-MS. TLC allowed the separation and quantification of Phospholipid (PL) classes and GC –MS allowed the separation and quantification of FAs. TGs and CHL were quantified. Also, cell viability and apoptosis were evaluated. PL class analysis showed significant alterations in phosphatidylethanolamine (PE), phosphatidylserine (PS) in the medium with LA1 or LA2, respectively and lysophosphatidylcholine (LPC) in the medium with OA1. GC-MS showed an increase of 18:2 in the treatment with LA. Further TG showed a decrease on LA2 treatment and viability and apoptosis assays showed that LA1 and LA2 stimulate cell growth and OA2 stimulated apoptosis, respectively. This work contributes to a better understanding of the influence of dietary FAs on the lipidome of T-47D cells and shows that alterations in cellular lipidome are associated to changes in cell number and apoptosis. These findings may lead to new perspectives in the identification of specific targets that may be helpful to understand the process behind breast cancer growth. However, more studies are necessary to conclude about how different lipid molecules promote these effects. |
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The effect of dietary fatty acid on breast cancer lipidomeBioquímicaFosfolípidosÁcidos gordosCancro da mamaCélulas epiteliaisBreast cancer is a complex heterogeneous disease and one of the leading causes of death among women. Many studies have tried to determine if saturated, monounsaturated and polyunsaturated fatty acids (FA) have different effects on the development of breast cancer. However, the role of different dietary FAs in lipidome of breast cancer cells is not completely understood. The aim of this work was to establish if lipidome of breast cancer T47-D cell line differs with different dietary FAs and correlate this findings to effects in proliferation and apoptosis. The lipid changes in lipidome induced by the presence of 1 μg/mL oleic acid (OA1) or 1 or 2 μg/mL linoleic (LA1 or LA2, respectively) acid in vitro were study through a lipidomic analysis, based on techniques such as TLC and GC-MS. TLC allowed the separation and quantification of Phospholipid (PL) classes and GC –MS allowed the separation and quantification of FAs. TGs and CHL were quantified. Also, cell viability and apoptosis were evaluated. PL class analysis showed significant alterations in phosphatidylethanolamine (PE), phosphatidylserine (PS) in the medium with LA1 or LA2, respectively and lysophosphatidylcholine (LPC) in the medium with OA1. GC-MS showed an increase of 18:2 in the treatment with LA. Further TG showed a decrease on LA2 treatment and viability and apoptosis assays showed that LA1 and LA2 stimulate cell growth and OA2 stimulated apoptosis, respectively. This work contributes to a better understanding of the influence of dietary FAs on the lipidome of T-47D cells and shows that alterations in cellular lipidome are associated to changes in cell number and apoptosis. These findings may lead to new perspectives in the identification of specific targets that may be helpful to understand the process behind breast cancer growth. However, more studies are necessary to conclude about how different lipid molecules promote these effects.O cancro da mama é uma doença heterogênea complexa e uma das principais causas de morte entre as mulheres. Muitos estudos têm tentado determinar se ácidos gordos (FA) saturados, monoinsaturados e polinsaturados têm efeitos diferentes sobre o desenvolvimento de cancro da mama. No entanto o papel dos diferentes FAs da dieta no lipidoma de células de cancro da mama não é completamente compreendido. O objetivo deste trabalho foi avaliar se o lipidoma da linha celular T-47D de cancro da mama se altera com diferentes FAs da dieta e correlacionar essas alterações com efeitos na proliferação e apoptose celulares. As alterações lipídicas induzidas pela presença de 1 ug / mL de ácido oleico (OA1) ou 1 ou 2 ug / ml de ácido linoleico (LA1 ou LA2, respetivamente) de ácido in vitro foram estudadas através de uma análise lipidómica com base em técnicas tais como TLC, para a separação e quantificação de classes de fosfolípidos (PLs) e GC-MS, para separação e quantificação dos FAs. Os triglicerídeos (TGs) e colesterol (CHL) totais foram quantificados utilizando métodos colorimétricos. Além disso, a viabilidade celular e a apoptose também foram avaliadas. A análise de classes de PL permitiu observar alterações significativas nas classes de fosfatidiletanolamina (PE), fosfatidilserina (PS) nas células suplementadas com LA1 e LA2, respetivamente, e lisofosfatidilcolina (LPC) nas células suplementadas com OA1. Observou-se ainda um aumento do FA 18:2 no tratamento com LA. Além disso, os TGs mostraram uma diminuição no tratamento com LA2. Os ensaios de viabilidade e apoptose mostraram que LA1 e LA2 estimulam o crescimento celular e que OA2 estimula a apoptose. Este trabalho contribui para uma melhor compreensão da influência dos FAs da dieta no lipidoma de células T-47D e mostra que as alterações no lipidoma celular estão associadas a um aumento do metabolismo e crescimento celular nas células suplementadas com LA e a um aumento da apoptose em células suplementadas com OA. Essas descobertas podem levar a novas perspetivas na identificação de estratégias específicas que podem ser úteis para compreender o processo envolvido na progressão do cancro da mama. No entanto, mais estudos são necessários para concluir sobre como os diferentes lípidos podem promover estes efeitos.Universidade de Aveiro2015-01-28T11:51:24Z2014-01-01T00:00:00Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10773/13294TID:201562626engCosta, Ana Cláudia Marques Lopes Soares dainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T11:24:08Zoai:ria.ua.pt:10773/13294Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:49:09.898687Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The effect of dietary fatty acid on breast cancer lipidome |
title |
The effect of dietary fatty acid on breast cancer lipidome |
spellingShingle |
The effect of dietary fatty acid on breast cancer lipidome Costa, Ana Cláudia Marques Lopes Soares da Bioquímica Fosfolípidos Ácidos gordos Cancro da mama Células epiteliais |
title_short |
The effect of dietary fatty acid on breast cancer lipidome |
title_full |
The effect of dietary fatty acid on breast cancer lipidome |
title_fullStr |
The effect of dietary fatty acid on breast cancer lipidome |
title_full_unstemmed |
The effect of dietary fatty acid on breast cancer lipidome |
title_sort |
The effect of dietary fatty acid on breast cancer lipidome |
author |
Costa, Ana Cláudia Marques Lopes Soares da |
author_facet |
Costa, Ana Cláudia Marques Lopes Soares da |
author_role |
author |
dc.contributor.author.fl_str_mv |
Costa, Ana Cláudia Marques Lopes Soares da |
dc.subject.por.fl_str_mv |
Bioquímica Fosfolípidos Ácidos gordos Cancro da mama Células epiteliais |
topic |
Bioquímica Fosfolípidos Ácidos gordos Cancro da mama Células epiteliais |
description |
Breast cancer is a complex heterogeneous disease and one of the leading causes of death among women. Many studies have tried to determine if saturated, monounsaturated and polyunsaturated fatty acids (FA) have different effects on the development of breast cancer. However, the role of different dietary FAs in lipidome of breast cancer cells is not completely understood. The aim of this work was to establish if lipidome of breast cancer T47-D cell line differs with different dietary FAs and correlate this findings to effects in proliferation and apoptosis. The lipid changes in lipidome induced by the presence of 1 μg/mL oleic acid (OA1) or 1 or 2 μg/mL linoleic (LA1 or LA2, respectively) acid in vitro were study through a lipidomic analysis, based on techniques such as TLC and GC-MS. TLC allowed the separation and quantification of Phospholipid (PL) classes and GC –MS allowed the separation and quantification of FAs. TGs and CHL were quantified. Also, cell viability and apoptosis were evaluated. PL class analysis showed significant alterations in phosphatidylethanolamine (PE), phosphatidylserine (PS) in the medium with LA1 or LA2, respectively and lysophosphatidylcholine (LPC) in the medium with OA1. GC-MS showed an increase of 18:2 in the treatment with LA. Further TG showed a decrease on LA2 treatment and viability and apoptosis assays showed that LA1 and LA2 stimulate cell growth and OA2 stimulated apoptosis, respectively. This work contributes to a better understanding of the influence of dietary FAs on the lipidome of T-47D cells and shows that alterations in cellular lipidome are associated to changes in cell number and apoptosis. These findings may lead to new perspectives in the identification of specific targets that may be helpful to understand the process behind breast cancer growth. However, more studies are necessary to conclude about how different lipid molecules promote these effects. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-01-01T00:00:00Z 2014 2015-01-28T11:51:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/13294 TID:201562626 |
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http://hdl.handle.net/10773/13294 |
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TID:201562626 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de Aveiro |
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Universidade de Aveiro |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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