Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and genotype characterization

Detalhes bibliográficos
Autor(a) principal: Pereira, Rui C.
Data de Publicação: 2011
Outros Autores: Gentili, Chiara, Cancedda, Ranieri, Azevedo, Helena S., Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/12936
Resumo: This chapter is intended to provide a summary of the current materials used in cell encapsulation technology as well as methods for evaluating the performance of cells encapsulated in a polymeric matrix. In particular, it describes the experimental procedure to prepare a hydrogel matrix based on natural polymers for encapsulating and culturing human articular chondrocytes with the interest in cartilage regeneration. Protocols to evaluate the viability, proliferation, differentiation, and matrix production of embedded cells are also described and include standard protocols such as the MTT and [3H] Thymidine assays, reverse transcription polymerase chain reaction (RT-PCR) technique, histology, and immunohistochemistry analysis. The assessment of cell distribution within the 3D hydrogel construct is also described using APoTome analysis.
id RCAP_234279744e1b7ebd92b3a1995f3783b1
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/12936
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and genotype characterizationNatural polymersHydrogelsGelationChondrocyte encapsulationCell culture and survival within 3D matrixCell-based technologyCartilage regenerationScience & TechnologyThis chapter is intended to provide a summary of the current materials used in cell encapsulation technology as well as methods for evaluating the performance of cells encapsulated in a polymeric matrix. In particular, it describes the experimental procedure to prepare a hydrogel matrix based on natural polymers for encapsulating and culturing human articular chondrocytes with the interest in cartilage regeneration. Protocols to evaluate the viability, proliferation, differentiation, and matrix production of embedded cells are also described and include standard protocols such as the MTT and [3H] Thymidine assays, reverse transcription polymerase chain reaction (RT-PCR) technique, histology, and immunohistochemistry analysis. The assessment of cell distribution within the 3D hydrogel construct is also described using APoTome analysis.(undefined)Springer Science+Business MediaUniversidade do MinhoPereira, Rui C.Gentili, ChiaraCancedda, RanieriAzevedo, Helena S.Reis, R. L.20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/12936eng978-1-60761-984-01064-374510.1007/978-1-60761-984-0_1121042972http://www.ncbi.nlm.nih.gov/pubmed/21042972info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:18:29Zoai:repositorium.sdum.uminho.pt:1822/12936Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:11:19.293079Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and genotype characterization
title Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and genotype characterization
spellingShingle Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and genotype characterization
Pereira, Rui C.
Natural polymers
Hydrogels
Gelation
Chondrocyte encapsulation
Cell culture and survival within 3D matrix
Cell-based technology
Cartilage regeneration
Science & Technology
title_short Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and genotype characterization
title_full Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and genotype characterization
title_fullStr Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and genotype characterization
title_full_unstemmed Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and genotype characterization
title_sort Encapsulation of human articular chondrocytes into 3D hydrogel: phenotype and genotype characterization
author Pereira, Rui C.
author_facet Pereira, Rui C.
Gentili, Chiara
Cancedda, Ranieri
Azevedo, Helena S.
Reis, R. L.
author_role author
author2 Gentili, Chiara
Cancedda, Ranieri
Azevedo, Helena S.
Reis, R. L.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pereira, Rui C.
Gentili, Chiara
Cancedda, Ranieri
Azevedo, Helena S.
Reis, R. L.
dc.subject.por.fl_str_mv Natural polymers
Hydrogels
Gelation
Chondrocyte encapsulation
Cell culture and survival within 3D matrix
Cell-based technology
Cartilage regeneration
Science & Technology
topic Natural polymers
Hydrogels
Gelation
Chondrocyte encapsulation
Cell culture and survival within 3D matrix
Cell-based technology
Cartilage regeneration
Science & Technology
description This chapter is intended to provide a summary of the current materials used in cell encapsulation technology as well as methods for evaluating the performance of cells encapsulated in a polymeric matrix. In particular, it describes the experimental procedure to prepare a hydrogel matrix based on natural polymers for encapsulating and culturing human articular chondrocytes with the interest in cartilage regeneration. Protocols to evaluate the viability, proliferation, differentiation, and matrix production of embedded cells are also described and include standard protocols such as the MTT and [3H] Thymidine assays, reverse transcription polymerase chain reaction (RT-PCR) technique, histology, and immunohistochemistry analysis. The assessment of cell distribution within the 3D hydrogel construct is also described using APoTome analysis.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/12936
url https://hdl.handle.net/1822/12936
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 978-1-60761-984-0
1064-3745
10.1007/978-1-60761-984-0_11
21042972
http://www.ncbi.nlm.nih.gov/pubmed/21042972
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Science+Business Media
publisher.none.fl_str_mv Springer Science+Business Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132545046020096

Registros relacionados