A gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.8/4067 |
Resumo: | In this work a fast and simple multi-target gas chromatography–mass spectrometry (GC–MS) method for the simultaneous detection and absolute quantification of amino acids, fatty acids and sterols in marine organisms is proposed. The methodology was applied to the characterization of the echinoderm Marthasterias glacialis Linnaeus spiny sea star extracts. The main factors influencing the extraction of target compounds were evaluated by using different extraction procedures, solvent systems and temperature conditions and a comparison with a reference technique was performed. The most suitable procedure, capable of successfully extract the three classes of target compounds, was ethanol as solvent at 40 1C under magnetic stirring. Good analytical parameters were obtained since calibrations curves for the 40 compounds under analysis (15 amino acids, 16 fatty acids and 9 sterols) showed regression coefficients (r 2 ) ranging from 0.9844 to 0.9978, with low RSD (from 0.00 to 9.45%), and detection limits varying from 0.03 to 15.40 mg/L. The RSD values for intra- and interday variations studies were also good (RSDo13.5%, for both) and recoveries were higher than 92%. Variation in samples from different harvests and origins and their chemical composition during the year is reported. The fact that no previous treatment of samples is required can make this a useful technique for metabolite profiling in marine organisms, among others, both in biomedical and nutritional studies. Moreover, due to the fast and robust character of the proposed method it seems to be suitable for the implementation as routine analysis. |
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A gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organismsMulti-target GC–MSMarine organismsMarthasterias glacialis LinnaeusAmino acidsFatty acidsSterolsIn this work a fast and simple multi-target gas chromatography–mass spectrometry (GC–MS) method for the simultaneous detection and absolute quantification of amino acids, fatty acids and sterols in marine organisms is proposed. The methodology was applied to the characterization of the echinoderm Marthasterias glacialis Linnaeus spiny sea star extracts. The main factors influencing the extraction of target compounds were evaluated by using different extraction procedures, solvent systems and temperature conditions and a comparison with a reference technique was performed. The most suitable procedure, capable of successfully extract the three classes of target compounds, was ethanol as solvent at 40 1C under magnetic stirring. Good analytical parameters were obtained since calibrations curves for the 40 compounds under analysis (15 amino acids, 16 fatty acids and 9 sterols) showed regression coefficients (r 2 ) ranging from 0.9844 to 0.9978, with low RSD (from 0.00 to 9.45%), and detection limits varying from 0.03 to 15.40 mg/L. The RSD values for intra- and interday variations studies were also good (RSDo13.5%, for both) and recoveries were higher than 92%. Variation in samples from different harvests and origins and their chemical composition during the year is reported. The fact that no previous treatment of samples is required can make this a useful technique for metabolite profiling in marine organisms, among others, both in biomedical and nutritional studies. Moreover, due to the fast and robust character of the proposed method it seems to be suitable for the implementation as routine analysis.ElsevierIC-OnlinePereira, David M.Vinholes, Julianade Pinho, Paula GuedesValentao, PatriciaMouga, TeresaTeixeira, NaterciaAndrade, Paula B.2019-09-06T10:17:59Z20122019-09-04T14:55:15Z2012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/4067engD.M. Pereira, et al., A gas chromatography–mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms, Talanta (2012), http://dx.doi.org/10.1016/j.talanta.2012.08.004WOS:000313773300052cv-prod-14997410.1016/j.talanta.2012.08.004metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:48:31Zoai:iconline.ipleiria.pt:10400.8/4067Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:48:01.729247Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
A gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms |
title |
A gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms |
spellingShingle |
A gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms Pereira, David M. Multi-target GC–MS Marine organisms Marthasterias glacialis Linnaeus Amino acids Fatty acids Sterols |
title_short |
A gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms |
title_full |
A gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms |
title_fullStr |
A gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms |
title_full_unstemmed |
A gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms |
title_sort |
A gas chromatography-mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms |
author |
Pereira, David M. |
author_facet |
Pereira, David M. Vinholes, Juliana de Pinho, Paula Guedes Valentao, Patricia Mouga, Teresa Teixeira, Natercia Andrade, Paula B. |
author_role |
author |
author2 |
Vinholes, Juliana de Pinho, Paula Guedes Valentao, Patricia Mouga, Teresa Teixeira, Natercia Andrade, Paula B. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
IC-Online |
dc.contributor.author.fl_str_mv |
Pereira, David M. Vinholes, Juliana de Pinho, Paula Guedes Valentao, Patricia Mouga, Teresa Teixeira, Natercia Andrade, Paula B. |
dc.subject.por.fl_str_mv |
Multi-target GC–MS Marine organisms Marthasterias glacialis Linnaeus Amino acids Fatty acids Sterols |
topic |
Multi-target GC–MS Marine organisms Marthasterias glacialis Linnaeus Amino acids Fatty acids Sterols |
description |
In this work a fast and simple multi-target gas chromatography–mass spectrometry (GC–MS) method for the simultaneous detection and absolute quantification of amino acids, fatty acids and sterols in marine organisms is proposed. The methodology was applied to the characterization of the echinoderm Marthasterias glacialis Linnaeus spiny sea star extracts. The main factors influencing the extraction of target compounds were evaluated by using different extraction procedures, solvent systems and temperature conditions and a comparison with a reference technique was performed. The most suitable procedure, capable of successfully extract the three classes of target compounds, was ethanol as solvent at 40 1C under magnetic stirring. Good analytical parameters were obtained since calibrations curves for the 40 compounds under analysis (15 amino acids, 16 fatty acids and 9 sterols) showed regression coefficients (r 2 ) ranging from 0.9844 to 0.9978, with low RSD (from 0.00 to 9.45%), and detection limits varying from 0.03 to 15.40 mg/L. The RSD values for intra- and interday variations studies were also good (RSDo13.5%, for both) and recoveries were higher than 92%. Variation in samples from different harvests and origins and their chemical composition during the year is reported. The fact that no previous treatment of samples is required can make this a useful technique for metabolite profiling in marine organisms, among others, both in biomedical and nutritional studies. Moreover, due to the fast and robust character of the proposed method it seems to be suitable for the implementation as routine analysis. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2012-01-01T00:00:00Z 2019-09-06T10:17:59Z 2019-09-04T14:55:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.8/4067 |
url |
http://hdl.handle.net/10400.8/4067 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
D.M. Pereira, et al., A gas chromatography–mass spectrometry multi-target method for the simultaneous analysis of three classes of metabolites in marine organisms, Talanta (2012), http://dx.doi.org/10.1016/j.talanta.2012.08.004 WOS:000313773300052 cv-prod-149974 10.1016/j.talanta.2012.08.004 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799136973656424448 |