HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star)

Detalhes bibliográficos
Autor(a) principal: Ferreres, Federico
Data de Publicação: 2010
Outros Autores: Pereira, David M., Gil-Izquierdo, Angel, Valentão, Patrícia, Botelho, João, Mouga, Teresa, Andrade, Paula B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/4064
Resumo: An HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling analysis was conducted on the marine echinoderm Marthasterias glacialis (spiny sea-star). Bio-guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV–Vis characteristics and MS spectra interpretation. No previous works addressed the MS analysis of carotenoids present in this organism. The purified carotenoid fraction displayed a strong cell proliferation inhibition against rat basophilic leukemia RBL-2H3 (IC25 5 268 mg/mL) cancer cell line. Against healthy V79 (rat lung fibroblasts (IC25 5411 mg/mL)) cell line, however, toxicity was lower, as it is desired for anti-cancer molecules. This study suggests that M. glacialis may constitute a good source of bioactive compounds that can be used as lead compounds for the pharmaceutical industry.
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spelling HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star)CarotenoidsCytotoxicHPLC-PAD-atmospheric pressure chemical ionization-MSMarthasterias glacialisAn HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling analysis was conducted on the marine echinoderm Marthasterias glacialis (spiny sea-star). Bio-guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV–Vis characteristics and MS spectra interpretation. No previous works addressed the MS analysis of carotenoids present in this organism. The purified carotenoid fraction displayed a strong cell proliferation inhibition against rat basophilic leukemia RBL-2H3 (IC25 5 268 mg/mL) cancer cell line. Against healthy V79 (rat lung fibroblasts (IC25 5411 mg/mL)) cell line, however, toxicity was lower, as it is desired for anti-cancer molecules. This study suggests that M. glacialis may constitute a good source of bioactive compounds that can be used as lead compounds for the pharmaceutical industry.IC-OnlineFerreres, FedericoPereira, David M.Gil-Izquierdo, AngelValentão, PatríciaBotelho, JoãoMouga, TeresaAndrade, Paula B.2019-09-06T09:09:45Z20102019-09-04T14:51:09Z2010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/4064engWOS:000281055900004cv-prod-14996410.1002/jssc.201000197metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:48:31Zoai:iconline.ipleiria.pt:10400.8/4064Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:48:01.583145Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star)
title HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star)
spellingShingle HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star)
Ferreres, Federico
Carotenoids
Cytotoxic
HPLC-PAD-atmospheric pressure chemical ionization-MS
Marthasterias glacialis
title_short HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star)
title_full HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star)
title_fullStr HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star)
title_full_unstemmed HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star)
title_sort HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star)
author Ferreres, Federico
author_facet Ferreres, Federico
Pereira, David M.
Gil-Izquierdo, Angel
Valentão, Patrícia
Botelho, João
Mouga, Teresa
Andrade, Paula B.
author_role author
author2 Pereira, David M.
Gil-Izquierdo, Angel
Valentão, Patrícia
Botelho, João
Mouga, Teresa
Andrade, Paula B.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Ferreres, Federico
Pereira, David M.
Gil-Izquierdo, Angel
Valentão, Patrícia
Botelho, João
Mouga, Teresa
Andrade, Paula B.
dc.subject.por.fl_str_mv Carotenoids
Cytotoxic
HPLC-PAD-atmospheric pressure chemical ionization-MS
Marthasterias glacialis
topic Carotenoids
Cytotoxic
HPLC-PAD-atmospheric pressure chemical ionization-MS
Marthasterias glacialis
description An HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling analysis was conducted on the marine echinoderm Marthasterias glacialis (spiny sea-star). Bio-guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV–Vis characteristics and MS spectra interpretation. No previous works addressed the MS analysis of carotenoids present in this organism. The purified carotenoid fraction displayed a strong cell proliferation inhibition against rat basophilic leukemia RBL-2H3 (IC25 5 268 mg/mL) cancer cell line. Against healthy V79 (rat lung fibroblasts (IC25 5411 mg/mL)) cell line, however, toxicity was lower, as it is desired for anti-cancer molecules. This study suggests that M. glacialis may constitute a good source of bioactive compounds that can be used as lead compounds for the pharmaceutical industry.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
2019-09-06T09:09:45Z
2019-09-04T14:51:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/4064
url http://hdl.handle.net/10400.8/4064
dc.language.iso.fl_str_mv eng
language eng
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cv-prod-149964
10.1002/jssc.201000197
dc.rights.driver.fl_str_mv metadata only access
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instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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