Cytotoxicity of Aspergillus Section Fumigati isolates recovered from protection devices used on waste sorting industry

Detalhes bibliográficos
Autor(a) principal: Viegas, Carla
Data de Publicação: 2022
Outros Autores: Twarużek, Magdalena, Dias, Marta, Carolino, Elisabete, Soszczyńska, Ewelina, Caetano, Liliana Aranha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/137369
Resumo: Funding Information: Funding: This research was funded by FCT—Fundação para a Ciência e a Tecnologia, I.P. (Portugal) for funding the EEA Grants Project “EXPO-Green”, by Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices” (IPL/2018/WasteFRPD_ESTeSL) and to Polish Minister of Science and Higher Education, under the program “Regional Initiative of Excellence” in 2019–2022 (Grant No. 008/RID/2018/19). Funding Information: This research was funded by FCT?Funda??o para a Ci?ncia e a Tecnologia, I.P. (Portugal) for funding the EEA Grants Project ?EXPO-Green?, by Instituto Polit?cnico de Lisboa, Lisbon, Portugal for funding the Project "Waste Workers? Exposure to Bioburden through Filtering Respiratory Protective Devices? (IPL/2018/WasteFRPD_ESTeSL) and to Polish Minister of Science and Higher Education, under the program ?Regional Initiative of Excellence? in 2019?2022 (Grant No. 008/RID/2018/19). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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spelling Cytotoxicity of Aspergillus Section Fumigati isolates recovered from protection devices used on waste sorting industryCytotoxicityMTT assayProtection devicesRisk assessmentWaste sorting environmentToxicologyHealth, Toxicology and MutagenesisFunding Information: Funding: This research was funded by FCT—Fundação para a Ciência e a Tecnologia, I.P. (Portugal) for funding the EEA Grants Project “EXPO-Green”, by Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices” (IPL/2018/WasteFRPD_ESTeSL) and to Polish Minister of Science and Higher Education, under the program “Regional Initiative of Excellence” in 2019–2022 (Grant No. 008/RID/2018/19). Funding Information: This research was funded by FCT?Funda??o para a Ci?ncia e a Tecnologia, I.P. (Portugal) for funding the EEA Grants Project ?EXPO-Green?, by Instituto Polit?cnico de Lisboa, Lisbon, Portugal for funding the Project "Waste Workers? Exposure to Bioburden through Filtering Respiratory Protective Devices? (IPL/2018/WasteFRPD_ESTeSL) and to Polish Minister of Science and Higher Education, under the program ?Regional Initiative of Excellence? in 2019?2022 (Grant No. 008/RID/2018/19). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.Safe working conditions must be guaranteed during waste sorting, which is crucial to maximizing recycling and reuse, in order to minimize workers’ exposure to chemical and biological hazards. This study determines the contribution of Aspergillus section Fumigati to the overall cytotoxicity of filtering respiratory protection devices (FRPD) and mechanic protection gloves (MPG) collected in 2019 from different workstations in one waste sorting industry in Portugal. The cytotoxicity of 133 Aspergillus section Fumigati isolates was determined as IC50 in human A549 epithelial lung cells and swine kidney cells, using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Aspergillus section Fumigati cytotoxicity results were compared with previous total cytotoxicity data from FRPD and MPG samples. A significant correlation was detected between the total cytotoxicity of samples and cytotoxicity of Aspergillus section Fumigati isolates in A549 cells (rS = −0.339, p = 0.030). The cytotoxicity of Aspergillus section Fumigati isolates explained 10.7% of the total cytotoxicity of the sample. On the basis of the comparison of cytotoxicity levels, it was possible to determine the contribution of Aspergillus section Fumigati isolates for the total cytotoxicity of protection devices used in the waste sorting industry. The results support in vitro toxicology as a relevant approach in risk assessments regarding cytotoxicity in passive sampling, and thus, useful in determining the contribution of relevant microbial contaminants to overall cytotoxicity. This approach can provide valuable answers in dose/response studies, and support innovations in risk characterization and their translation into occupational policies.Comprehensive Health Research Centre (CHRC) - Pólo ENSPCentro de Investigação em Saúde Pública (CISP/PHRC)Escola Nacional de Saúde Pública (ENSP)RUNViegas, CarlaTwarużek, MagdalenaDias, MartaCarolino, ElisabeteSoszczyńska, EwelinaCaetano, Liliana Aranha2022-05-03T22:49:04Z2022-022022-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/137369eng2072-6651PURE: 43565830https://doi.org/10.3390/toxins14020070info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:14:54Zoai:run.unl.pt:10362/137369Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:48:49.806802Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cytotoxicity of Aspergillus Section Fumigati isolates recovered from protection devices used on waste sorting industry
title Cytotoxicity of Aspergillus Section Fumigati isolates recovered from protection devices used on waste sorting industry
spellingShingle Cytotoxicity of Aspergillus Section Fumigati isolates recovered from protection devices used on waste sorting industry
Viegas, Carla
Cytotoxicity
MTT assay
Protection devices
Risk assessment
Waste sorting environment
Toxicology
Health, Toxicology and Mutagenesis
title_short Cytotoxicity of Aspergillus Section Fumigati isolates recovered from protection devices used on waste sorting industry
title_full Cytotoxicity of Aspergillus Section Fumigati isolates recovered from protection devices used on waste sorting industry
title_fullStr Cytotoxicity of Aspergillus Section Fumigati isolates recovered from protection devices used on waste sorting industry
title_full_unstemmed Cytotoxicity of Aspergillus Section Fumigati isolates recovered from protection devices used on waste sorting industry
title_sort Cytotoxicity of Aspergillus Section Fumigati isolates recovered from protection devices used on waste sorting industry
author Viegas, Carla
author_facet Viegas, Carla
Twarużek, Magdalena
Dias, Marta
Carolino, Elisabete
Soszczyńska, Ewelina
Caetano, Liliana Aranha
author_role author
author2 Twarużek, Magdalena
Dias, Marta
Carolino, Elisabete
Soszczyńska, Ewelina
Caetano, Liliana Aranha
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Comprehensive Health Research Centre (CHRC) - Pólo ENSP
Centro de Investigação em Saúde Pública (CISP/PHRC)
Escola Nacional de Saúde Pública (ENSP)
RUN
dc.contributor.author.fl_str_mv Viegas, Carla
Twarużek, Magdalena
Dias, Marta
Carolino, Elisabete
Soszczyńska, Ewelina
Caetano, Liliana Aranha
dc.subject.por.fl_str_mv Cytotoxicity
MTT assay
Protection devices
Risk assessment
Waste sorting environment
Toxicology
Health, Toxicology and Mutagenesis
topic Cytotoxicity
MTT assay
Protection devices
Risk assessment
Waste sorting environment
Toxicology
Health, Toxicology and Mutagenesis
description Funding Information: Funding: This research was funded by FCT—Fundação para a Ciência e a Tecnologia, I.P. (Portugal) for funding the EEA Grants Project “EXPO-Green”, by Instituto Politécnico de Lisboa, Lisbon, Portugal for funding the Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices” (IPL/2018/WasteFRPD_ESTeSL) and to Polish Minister of Science and Higher Education, under the program “Regional Initiative of Excellence” in 2019–2022 (Grant No. 008/RID/2018/19). Funding Information: This research was funded by FCT?Funda??o para a Ci?ncia e a Tecnologia, I.P. (Portugal) for funding the EEA Grants Project ?EXPO-Green?, by Instituto Polit?cnico de Lisboa, Lisbon, Portugal for funding the Project "Waste Workers? Exposure to Bioburden through Filtering Respiratory Protective Devices? (IPL/2018/WasteFRPD_ESTeSL) and to Polish Minister of Science and Higher Education, under the program ?Regional Initiative of Excellence? in 2019?2022 (Grant No. 008/RID/2018/19). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
publishDate 2022
dc.date.none.fl_str_mv 2022-05-03T22:49:04Z
2022-02
2022-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/137369
url http://hdl.handle.net/10362/137369
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2072-6651
PURE: 43565830
https://doi.org/10.3390/toxins14020070
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