Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry

Detalhes bibliográficos
Autor(a) principal: Viegas, Carla
Data de Publicação: 2022
Outros Autores: Twarużek, Magdalena, Dias, Marta, Carolino, Elisabete, Soszczyńska, Ewelina, Caetano, Liliana Aranha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/14208
Resumo: FCT_EEA Grants Project “EXPO-Green”.
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spelling Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industryOccupational healthOccupational exposureCitotoxicityProtection devicesMTT assayRisk assessmentWaste sorting environmentFCT_EEA Grants Project “EXPO-Green”Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices”IPL/2018/WasteFRPD_ESTeSLFCT_EEA Grants Project “EXPO-Green”.Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices” (IPL/2018/WasteFRPD_ESTeSL).Safe working conditions must be guaranteed during waste sorting, which is crucial to maximizing recycling and reuse, in order to minimize workers’ exposure to chemical and biological hazards. This study determines the contribution of Aspergillus section Fumigati to the overall cytotoxicity of filtering respiratory protection devices (FRPD) and mechanic protection gloves (MPG) collected in 2019 from different workstations in one waste sorting industry in Portugal. The cytotoxicity of 133 Aspergillus section Fumigati isolates was determined as IC50 in human A549 epithelial lung cells and swine kidney cells, using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Aspergillus section Fumigati cytotoxicity results were compared with previous total cytotoxicity data from FRPD and MPG samples. A significant correlation was detected between the total cytotoxicity of samples and cytotoxicity of Aspergillus section Fumigati isolates in A549 cells (rS = −0.339, p = 0.030). The cytotoxicity of Aspergillus section Fumigati isolates explained 10.7% of the total cytotoxicity of the sample. On the basis of the comparison of cytotoxicity levels, it was possible to determine the contribution of Aspergillus section Fumigati isolates for the total cytotoxicity of protection devices used in the waste sorting industry. The results support in vitro toxicology as a relevant approach in risk assessments regarding cytotoxicity in passive sampling, and thus, useful in determining the contribution of relevant microbial contaminants to overall cytotoxicity. This approach can provide valuable answers in dose/response studies, and support innovations in risk characterization and their translation into occupational policies.MDPIRCIPLViegas, CarlaTwarużek, MagdalenaDias, MartaCarolino, ElisabeteSoszczyńska, EwelinaCaetano, Liliana Aranha2022-01-26T13:16:37Z2022-012022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/14208engViegas C, Twarużek M, Dias M, Carolino E, Soszczyńska E, Caetano LA. Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry. Toxins. 2022;14(2):70.10.3390/toxins14020070info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:10:03Zoai:repositorio.ipl.pt:10400.21/14208Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:22:03.549273Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry
title Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry
spellingShingle Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry
Viegas, Carla
Occupational health
Occupational exposure
Citotoxicity
Protection devices
MTT assay
Risk assessment
Waste sorting environment
FCT_EEA Grants Project “EXPO-Green”
Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices”
IPL/2018/WasteFRPD_ESTeSL
title_short Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry
title_full Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry
title_fullStr Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry
title_full_unstemmed Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry
title_sort Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry
author Viegas, Carla
author_facet Viegas, Carla
Twarużek, Magdalena
Dias, Marta
Carolino, Elisabete
Soszczyńska, Ewelina
Caetano, Liliana Aranha
author_role author
author2 Twarużek, Magdalena
Dias, Marta
Carolino, Elisabete
Soszczyńska, Ewelina
Caetano, Liliana Aranha
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Viegas, Carla
Twarużek, Magdalena
Dias, Marta
Carolino, Elisabete
Soszczyńska, Ewelina
Caetano, Liliana Aranha
dc.subject.por.fl_str_mv Occupational health
Occupational exposure
Citotoxicity
Protection devices
MTT assay
Risk assessment
Waste sorting environment
FCT_EEA Grants Project “EXPO-Green”
Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices”
IPL/2018/WasteFRPD_ESTeSL
topic Occupational health
Occupational exposure
Citotoxicity
Protection devices
MTT assay
Risk assessment
Waste sorting environment
FCT_EEA Grants Project “EXPO-Green”
Project "Waste Workers’ Exposure to Bioburden through Filtering Respiratory Protective Devices”
IPL/2018/WasteFRPD_ESTeSL
description FCT_EEA Grants Project “EXPO-Green”.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-26T13:16:37Z
2022-01
2022-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/14208
url http://hdl.handle.net/10400.21/14208
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Viegas C, Twarużek M, Dias M, Carolino E, Soszczyńska E, Caetano LA. Cytotoxicity of Aspergillus section fumigati isolates recovered from protection devices used on waste sorting industry. Toxins. 2022;14(2):70.
10.3390/toxins14020070
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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