Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity.

Detalhes bibliográficos
Autor(a) principal: Cabrita, Ana
Data de Publicação: 2022
Outros Autores: Medeiros, Alexandra M, Pereira, Telmo, Rodrigues, António Sebastião, Kranendonk, Michel, Mendes, César S
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/141448
Resumo: 752891, GEMiNI to CSM), Fundac¸ a˜ o para a Cieˆ ncia e a Tecnologia (FCT) (PTDC/ BIA-COM/0151/2020, iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), and LS4FUTURE (LA/P/ 0087/2020) to CSM). AM was supported by a doctoral fellowship from FCT (PD/BD/128445/2017). ASR and MK were partially supported by grant UID/BIM/0009/2020 for the Research Center for Toxicogenomics and Human Health - (ToxOmics) from FCT, Portugal.
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spelling Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity.EntomologyNeurotoxicology752891, GEMiNI to CSM), Fundac¸ a˜ o para a Cieˆ ncia e a Tecnologia (FCT) (PTDC/ BIA-COM/0151/2020, iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), and LS4FUTURE (LA/P/ 0087/2020) to CSM). AM was supported by a doctoral fellowship from FCT (PD/BD/128445/2017). ASR and MK were partially supported by grant UID/BIM/0009/2020 for the Research Center for Toxicogenomics and Human Health - (ToxOmics) from FCT, Portugal.Adequate alternatives to conventional animal testing are needed to study developmental neurotoxicity (DNT). Here, we used kinematic analysis to assess DNT of known (toluene (TOL) and chlorpyrifos (CPS)) and putative (β-N-methylamino-L-alanine (BMAA)) neurotoxic compounds. Drosophila melanogaster was exposed to these compounds during development and evaluated for survival and adult kinematic parameters using the FlyWalker system, a kinematics evaluation method. At concentrations that do not induce general toxicity, the solvent DMSO had a significant effect on kinematic parameters. Moreover, while TOL did not significantly induce lethality or kinematic dysfunction, CPS not only induced developmental lethality but also significantly impaired coordination in comparison to DMSO. Interestingly, BMAA, which was not lethal during development, induced motor decay in young adult animals, phenotypically resembling aged flies, an effect later attenuated upon aging. Furthermore, BMAA induced abnormal development of leg motor neuron projections. Our results suggest that our kinematic approach can assess potential DNT of chemical compounds.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)iNOVA4Health - pólo NMSCentre for Toxicogenomics and Human Health (ToxOmics)RUNCabrita, AnaMedeiros, Alexandra MPereira, TelmoRodrigues, António SebastiãoKranendonk, MichelMendes, César S2022-07-05T22:30:30Z2022-07-152022-07-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/141448eng2589-0042PURE: 45153864https://doi.org/10.1016/j.isci.2022.104541info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:18:49Zoai:run.unl.pt:10362/141448Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:50:00.277921Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity.
title Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity.
spellingShingle Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity.
Cabrita, Ana
Entomology
Neurotoxicology
title_short Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity.
title_full Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity.
title_fullStr Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity.
title_full_unstemmed Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity.
title_sort Motor dysfunction in Drosophila melanogaster as a biomarker for developmental neurotoxicity.
author Cabrita, Ana
author_facet Cabrita, Ana
Medeiros, Alexandra M
Pereira, Telmo
Rodrigues, António Sebastião
Kranendonk, Michel
Mendes, César S
author_role author
author2 Medeiros, Alexandra M
Pereira, Telmo
Rodrigues, António Sebastião
Kranendonk, Michel
Mendes, César S
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)
iNOVA4Health - pólo NMS
Centre for Toxicogenomics and Human Health (ToxOmics)
RUN
dc.contributor.author.fl_str_mv Cabrita, Ana
Medeiros, Alexandra M
Pereira, Telmo
Rodrigues, António Sebastião
Kranendonk, Michel
Mendes, César S
dc.subject.por.fl_str_mv Entomology
Neurotoxicology
topic Entomology
Neurotoxicology
description 752891, GEMiNI to CSM), Fundac¸ a˜ o para a Cieˆ ncia e a Tecnologia (FCT) (PTDC/ BIA-COM/0151/2020, iNOVA4Health (UIDB/04462/2020 and UIDP/04462/2020), and LS4FUTURE (LA/P/ 0087/2020) to CSM). AM was supported by a doctoral fellowship from FCT (PD/BD/128445/2017). ASR and MK were partially supported by grant UID/BIM/0009/2020 for the Research Center for Toxicogenomics and Human Health - (ToxOmics) from FCT, Portugal.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-05T22:30:30Z
2022-07-15
2022-07-15T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/141448
url http://hdl.handle.net/10362/141448
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2589-0042
PURE: 45153864
https://doi.org/10.1016/j.isci.2022.104541
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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