Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS

Detalhes bibliográficos
Autor(a) principal: Garcia, Juliana
Data de Publicação: 2015
Outros Autores: Oliveira, Ana Paula Leandro, Pinho, P. Guedes de, Freitas, Victor, Carvalho, Alexandra T.P., Baptista, Paula, Pereira, Eric Carvalho, Bastos, Maria de Lourdes, Carvalho, Félix
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10198/16134
Resumo: Amanita phalloides is a toxic mushroom responsible for the majority of deaths occurring after mushrooms ingestion, mainly due to amatoxins. In the present study the contents and distribution of the major amatoxins and phallotoxins in different tissues of A. phalloides from two different sites of Portugal were analyzed by liquid chromatography (LC) coupled to diode array (DAD) and mass spectrometry (MS) detection. The main toxins were separated by LC and its chemical structures confirmed by MS. a-Amanitin contents in caps, stipe and volva tissues were quantified by RP-HPLC. The results show that caps have the highest content of amatoxins, whereas the volva was richest in phallotoxins. Moreover variability in the toxins composition from different geographic sites was also observed. This study provides for the first time the content of toxins in A. phalloides from Portugal.
id RCAP_2612d55aed9d83e5525ed05d37935161
oai_identifier_str oai:bibliotecadigital.ipb.pt:10198/16134
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MSAmanita phalloidesMass spectrometryReversed-phase high performance liquid chromatographyToxinsα-amanitinAmanita phalloides is a toxic mushroom responsible for the majority of deaths occurring after mushrooms ingestion, mainly due to amatoxins. In the present study the contents and distribution of the major amatoxins and phallotoxins in different tissues of A. phalloides from two different sites of Portugal were analyzed by liquid chromatography (LC) coupled to diode array (DAD) and mass spectrometry (MS) detection. The main toxins were separated by LC and its chemical structures confirmed by MS. a-Amanitin contents in caps, stipe and volva tissues were quantified by RP-HPLC. The results show that caps have the highest content of amatoxins, whereas the volva was richest in phallotoxins. Moreover variability in the toxins composition from different geographic sites was also observed. This study provides for the first time the content of toxins in A. phalloides from Portugal.Authors are grateful to Dr Zélia dos Santos Azevedo, Faculty of Sciences, University of Porto, who loaned LC/DAD-ESI/MS and for all technical assistance. The authors also are grateful for the help of the Foundation for the Science and Technology (FCT, Portugal) for financial support and also thank FCT for doctoral grant SFRH/BD/74979/2010.Biblioteca Digital do IPBGarcia, JulianaOliveira, Ana Paula LeandroPinho, P. Guedes deFreitas, VictorCarvalho, Alexandra T.P.Baptista, PaulaPereira, Eric CarvalhoBastos, Maria de LourdesCarvalho, Félix2018-01-19T10:00:00Z20152015-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10198/16134engGarcia, Juliana; Oliveira, Ana; Guedes de Pinho, Paula; Freitas, Victor; Carvalho, Alexandra; Baptista, Paula; Pereira, Eric; Bastos, Maria de Lourdes; Carvalho, Félix (2015). Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS. Mycologia. ISSN 0027-5514. 107, p. 679-6870027-551410.3852/14-253info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-21T10:37:13Zoai:bibliotecadigital.ipb.pt:10198/16134Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:05:25.157974Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS
title Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS
spellingShingle Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS
Garcia, Juliana
Amanita phalloides
Mass spectrometry
Reversed-phase high performance liquid chromatography
Toxins
α-amanitin
title_short Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS
title_full Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS
title_fullStr Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS
title_full_unstemmed Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS
title_sort Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS
author Garcia, Juliana
author_facet Garcia, Juliana
Oliveira, Ana Paula Leandro
Pinho, P. Guedes de
Freitas, Victor
Carvalho, Alexandra T.P.
Baptista, Paula
Pereira, Eric Carvalho
Bastos, Maria de Lourdes
Carvalho, Félix
author_role author
author2 Oliveira, Ana Paula Leandro
Pinho, P. Guedes de
Freitas, Victor
Carvalho, Alexandra T.P.
Baptista, Paula
Pereira, Eric Carvalho
Bastos, Maria de Lourdes
Carvalho, Félix
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Biblioteca Digital do IPB
dc.contributor.author.fl_str_mv Garcia, Juliana
Oliveira, Ana Paula Leandro
Pinho, P. Guedes de
Freitas, Victor
Carvalho, Alexandra T.P.
Baptista, Paula
Pereira, Eric Carvalho
Bastos, Maria de Lourdes
Carvalho, Félix
dc.subject.por.fl_str_mv Amanita phalloides
Mass spectrometry
Reversed-phase high performance liquid chromatography
Toxins
α-amanitin
topic Amanita phalloides
Mass spectrometry
Reversed-phase high performance liquid chromatography
Toxins
α-amanitin
description Amanita phalloides is a toxic mushroom responsible for the majority of deaths occurring after mushrooms ingestion, mainly due to amatoxins. In the present study the contents and distribution of the major amatoxins and phallotoxins in different tissues of A. phalloides from two different sites of Portugal were analyzed by liquid chromatography (LC) coupled to diode array (DAD) and mass spectrometry (MS) detection. The main toxins were separated by LC and its chemical structures confirmed by MS. a-Amanitin contents in caps, stipe and volva tissues were quantified by RP-HPLC. The results show that caps have the highest content of amatoxins, whereas the volva was richest in phallotoxins. Moreover variability in the toxins composition from different geographic sites was also observed. This study provides for the first time the content of toxins in A. phalloides from Portugal.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01T00:00:00Z
2018-01-19T10:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10198/16134
url http://hdl.handle.net/10198/16134
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Garcia, Juliana; Oliveira, Ana; Guedes de Pinho, Paula; Freitas, Victor; Carvalho, Alexandra; Baptista, Paula; Pereira, Eric; Bastos, Maria de Lourdes; Carvalho, Félix (2015). Determination of amatoxins and phallotoxins in Amanita phalloides mushrooms from northeastern Portugal by HPLC-DAD-MS. Mycologia. ISSN 0027-5514. 107, p. 679-687
0027-5514
10.3852/14-253
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1817553400001200128

Registros relacionados