Crystal violet staining alone is not adequate to assess synergism or antagonism in multi-species biofilms of bacteria associated with bacterial vaginosis

Detalhes bibliográficos
Autor(a) principal: Castro, Joana Isabel Reis
Data de Publicação: 2022
Outros Autores: Lima, Ângela Martins, Sousa, Lúcia, Rosca, Aliona, Muzny, Christina A., Cerca, Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/75734
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021. 795797/full#supplementary-material
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spelling Crystal violet staining alone is not adequate to assess synergism or antagonism in multi-species biofilms of bacteria associated with bacterial vaginosisAnaerobic bacteriaBacterial vaginosisBiofilm quantificationCrystal violet stainingMicrotiter platesThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021. 795797/full#supplementary-materialBacterial Vaginosis (BV) involves the presence of a multi-species biofilm adhered to vaginal epithelial cells, but its in-depth study has been limited due to the complexity of the bacterial community, which makes the design of in vitro models challenging. Perhaps the most common experimental technique to quantify biofilms is the crystal violet (CV) staining method. Despite its widespread utilization, the CV method is not without flaws. While biofilm CV quantification within the same strain in different conditions is normally accepted, assessing multi-species biofilms formation by CV staining might provide significant bias. For BV research, determining possible synergism or antagonism between species is a fundamental step for assessing the roles of individual species in BV development. Herein, we provide our perspective on how CV fails to properly quantify an in vitro triple-species biofilm composed of Gardnerella vaginalis, Fannyhessea (Atopobium) vaginae, and Prevotella bivia, three common BV-associated bacteria thought to play key roles in incident BV pathogenesis. We compared the CV method with total colony forming units (CFU) and fluorescence microscopy cell count methods. Not surprisingly, when comparing single-species biofilms, the relationship between biofilm biomass, total number of cells, and total cultivable cells was very different between each tested method, and also varied with the time of incubation. Thus, despite its wide utilization for single-species biofilm quantification, the CV method should not be considered for accurate quantification of multi-species biofilms in BV pathogenesis research.NIAID -National Institute of Allergy and Infectious Diseases(POCI-01-0145-FEDER-028271)info:eu-repo/semantics/publishedVersionFrontiers MediaUniversidade do MinhoCastro, Joana Isabel ReisLima, Ângela MartinsSousa, LúciaRosca, AlionaMuzny, Christina A.Cerca, Nuno2022-01-052022-01-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75734engCastro, Joana; Lima, Â.; Sousa, Lúcia; Rosca, A.; Muzny, Christina A.; Cerca, Nuno, Crystal violet staining alone is not adequate to assess synergism or antagonism in multi-species biofilms of bacteria associated with bacterial vaginosis. Frontiers in Cellular and Infection Microbiology, 11(795797), 20222235-298810.3389/fcimb.2021.79579735071046https://www.frontiersin.org/journals/cellular-and-infection-microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:28:40Zoai:repositorium.sdum.uminho.pt:1822/75734Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:23:31.500821Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Crystal violet staining alone is not adequate to assess synergism or antagonism in multi-species biofilms of bacteria associated with bacterial vaginosis
title Crystal violet staining alone is not adequate to assess synergism or antagonism in multi-species biofilms of bacteria associated with bacterial vaginosis
spellingShingle Crystal violet staining alone is not adequate to assess synergism or antagonism in multi-species biofilms of bacteria associated with bacterial vaginosis
Castro, Joana Isabel Reis
Anaerobic bacteria
Bacterial vaginosis
Biofilm quantification
Crystal violet staining
Microtiter plates
title_short Crystal violet staining alone is not adequate to assess synergism or antagonism in multi-species biofilms of bacteria associated with bacterial vaginosis
title_full Crystal violet staining alone is not adequate to assess synergism or antagonism in multi-species biofilms of bacteria associated with bacterial vaginosis
title_fullStr Crystal violet staining alone is not adequate to assess synergism or antagonism in multi-species biofilms of bacteria associated with bacterial vaginosis
title_full_unstemmed Crystal violet staining alone is not adequate to assess synergism or antagonism in multi-species biofilms of bacteria associated with bacterial vaginosis
title_sort Crystal violet staining alone is not adequate to assess synergism or antagonism in multi-species biofilms of bacteria associated with bacterial vaginosis
author Castro, Joana Isabel Reis
author_facet Castro, Joana Isabel Reis
Lima, Ângela Martins
Sousa, Lúcia
Rosca, Aliona
Muzny, Christina A.
Cerca, Nuno
author_role author
author2 Lima, Ângela Martins
Sousa, Lúcia
Rosca, Aliona
Muzny, Christina A.
Cerca, Nuno
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Castro, Joana Isabel Reis
Lima, Ângela Martins
Sousa, Lúcia
Rosca, Aliona
Muzny, Christina A.
Cerca, Nuno
dc.subject.por.fl_str_mv Anaerobic bacteria
Bacterial vaginosis
Biofilm quantification
Crystal violet staining
Microtiter plates
topic Anaerobic bacteria
Bacterial vaginosis
Biofilm quantification
Crystal violet staining
Microtiter plates
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fcimb.2021. 795797/full#supplementary-material
publishDate 2022
dc.date.none.fl_str_mv 2022-01-05
2022-01-05T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/75734
url http://hdl.handle.net/1822/75734
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Castro, Joana; Lima, Â.; Sousa, Lúcia; Rosca, A.; Muzny, Christina A.; Cerca, Nuno, Crystal violet staining alone is not adequate to assess synergism or antagonism in multi-species biofilms of bacteria associated with bacterial vaginosis. Frontiers in Cellular and Infection Microbiology, 11(795797), 2022
2235-2988
10.3389/fcimb.2021.795797
35071046
https://www.frontiersin.org/journals/cellular-and-infection-microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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