Polymicrobial biofilms in cystic fibrosis – the role of atypical bacteria in the consortia and impact in antibiotic treatment

Detalhes bibliográficos
Autor(a) principal: Lopes, Susana Patrícia
Data de Publicação: 2014
Outros Autores: Azevedo, N. F., Pereira, Maria Olívia
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/31572
Resumo: Cystic Fibrosis (CF) is characterized by high rates of morbidity and mortality caused by pulmonary microbial infections. Pseudomonas aeruginosa is typically the prevailing pathogen in the airways of CF patients. However, an emergent and diverse microbial community inhabiting CF lungs has been disclosed, but how it interacts and contributes to the polymicrobial consortia with CF-common pathogens is still to be revealed. The mail goal of this study was to address the behavior of two CF-atypical bacteria, Inquilinus limosus (IL) and Dolosigranulum pigrum (DP), when associated to P. aeruginosa (PA) under oxygen-atmospheres resembling CF airways. For this, those bacteria were grown in dual- and three-species populations with P. aeruginosa in variable oxygen conditions and biofilms were thoroughly characterized for biomass, activity, CFU numbers, antibiotic resistance profiles and relative distributions of bacterial populations. Dual-species consortia were of difficult eradication, with most antibiotics being ineffective in reducing biofilm-bacteria, particularly under low-oxygen atmospheres. Regarding microbial composition, these biofilms presented similar bacterial proportions, whereas P. aeruginosa and D. pigrum dominated the three-species consortia, with I. limosus being the smallest representative population. In general, biofilm compositions changed as a result of antibiotic treatment, with alterations being dependent on the antibiotic, concentration and oxygen condition implemented. P. aeruginosa and I. limosus dual-biofilms exhibited higher antibiotic resistance, with I. limosus persisting and occupying a significant portion together with P. aeruginosa in the overall biofilm after antibiotic treatment. Interestingly, the three-species biofilms displayed higher sensitivity, with D. pigrum and/or P. aeruginosa dominating and I. limosus populations declining in most cases. This suggests that the preponderance of D. pigrum in the biofilm was decisive to decrease I. limosus and lead to an increase in overall sensitivity of the biofilm to a large number of antibiotics. PNA FISH allowed the direct observation of the location and distribution of the three-species species within the biofilms, corroborating the dominance of D. pigrum and P. aeruginosa within the mixed-species consortia and facilitating the understanding of the real complex interactions among the bacterial species. Data highlighted that emergent species are able to establish polymicrobial consortia with common pathogens in the airways of CF patients, modulating different social activities into those communities and impacting the CF therapeutics.
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spelling Polymicrobial biofilms in cystic fibrosis – the role of atypical bacteria in the consortia and impact in antibiotic treatmentCystic fibrosisPolymicrobial infectionBiofilmInquilinus limosusDolosigranulum pigrumCystic Fibrosis (CF) is characterized by high rates of morbidity and mortality caused by pulmonary microbial infections. Pseudomonas aeruginosa is typically the prevailing pathogen in the airways of CF patients. However, an emergent and diverse microbial community inhabiting CF lungs has been disclosed, but how it interacts and contributes to the polymicrobial consortia with CF-common pathogens is still to be revealed. The mail goal of this study was to address the behavior of two CF-atypical bacteria, Inquilinus limosus (IL) and Dolosigranulum pigrum (DP), when associated to P. aeruginosa (PA) under oxygen-atmospheres resembling CF airways. For this, those bacteria were grown in dual- and three-species populations with P. aeruginosa in variable oxygen conditions and biofilms were thoroughly characterized for biomass, activity, CFU numbers, antibiotic resistance profiles and relative distributions of bacterial populations. Dual-species consortia were of difficult eradication, with most antibiotics being ineffective in reducing biofilm-bacteria, particularly under low-oxygen atmospheres. Regarding microbial composition, these biofilms presented similar bacterial proportions, whereas P. aeruginosa and D. pigrum dominated the three-species consortia, with I. limosus being the smallest representative population. In general, biofilm compositions changed as a result of antibiotic treatment, with alterations being dependent on the antibiotic, concentration and oxygen condition implemented. P. aeruginosa and I. limosus dual-biofilms exhibited higher antibiotic resistance, with I. limosus persisting and occupying a significant portion together with P. aeruginosa in the overall biofilm after antibiotic treatment. Interestingly, the three-species biofilms displayed higher sensitivity, with D. pigrum and/or P. aeruginosa dominating and I. limosus populations declining in most cases. This suggests that the preponderance of D. pigrum in the biofilm was decisive to decrease I. limosus and lead to an increase in overall sensitivity of the biofilm to a large number of antibiotics. PNA FISH allowed the direct observation of the location and distribution of the three-species species within the biofilms, corroborating the dominance of D. pigrum and P. aeruginosa within the mixed-species consortia and facilitating the understanding of the real complex interactions among the bacterial species. Data highlighted that emergent species are able to establish polymicrobial consortia with common pathogens in the airways of CF patients, modulating different social activities into those communities and impacting the CF therapeutics.Universidade do MinhoLopes, Susana PatríciaAzevedo, N. F.Pereira, Maria Olívia20142014-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/31572engLopes, Susana Patrícia; Azevedo, N. F.; Pereira, M. O., Polymicrobial biofilms in cystic fibrosis – the role of atypical bacteria in the consortia and impact in antibiotic treatment. ICAR 2014 - III International Conference on Antimicrobial Research. Madrid, Spain, Oct. 1-3, 231-2312014.http://www.icar-2014.org/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T07:30:51Zoai:repositorium.sdum.uminho.pt:1822/31572Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T07:30:51Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Polymicrobial biofilms in cystic fibrosis – the role of atypical bacteria in the consortia and impact in antibiotic treatment
title Polymicrobial biofilms in cystic fibrosis – the role of atypical bacteria in the consortia and impact in antibiotic treatment
spellingShingle Polymicrobial biofilms in cystic fibrosis – the role of atypical bacteria in the consortia and impact in antibiotic treatment
Lopes, Susana Patrícia
Cystic fibrosis
Polymicrobial infection
Biofilm
Inquilinus limosus
Dolosigranulum pigrum
title_short Polymicrobial biofilms in cystic fibrosis – the role of atypical bacteria in the consortia and impact in antibiotic treatment
title_full Polymicrobial biofilms in cystic fibrosis – the role of atypical bacteria in the consortia and impact in antibiotic treatment
title_fullStr Polymicrobial biofilms in cystic fibrosis – the role of atypical bacteria in the consortia and impact in antibiotic treatment
title_full_unstemmed Polymicrobial biofilms in cystic fibrosis – the role of atypical bacteria in the consortia and impact in antibiotic treatment
title_sort Polymicrobial biofilms in cystic fibrosis – the role of atypical bacteria in the consortia and impact in antibiotic treatment
author Lopes, Susana Patrícia
author_facet Lopes, Susana Patrícia
Azevedo, N. F.
Pereira, Maria Olívia
author_role author
author2 Azevedo, N. F.
Pereira, Maria Olívia
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lopes, Susana Patrícia
Azevedo, N. F.
Pereira, Maria Olívia
dc.subject.por.fl_str_mv Cystic fibrosis
Polymicrobial infection
Biofilm
Inquilinus limosus
Dolosigranulum pigrum
topic Cystic fibrosis
Polymicrobial infection
Biofilm
Inquilinus limosus
Dolosigranulum pigrum
description Cystic Fibrosis (CF) is characterized by high rates of morbidity and mortality caused by pulmonary microbial infections. Pseudomonas aeruginosa is typically the prevailing pathogen in the airways of CF patients. However, an emergent and diverse microbial community inhabiting CF lungs has been disclosed, but how it interacts and contributes to the polymicrobial consortia with CF-common pathogens is still to be revealed. The mail goal of this study was to address the behavior of two CF-atypical bacteria, Inquilinus limosus (IL) and Dolosigranulum pigrum (DP), when associated to P. aeruginosa (PA) under oxygen-atmospheres resembling CF airways. For this, those bacteria were grown in dual- and three-species populations with P. aeruginosa in variable oxygen conditions and biofilms were thoroughly characterized for biomass, activity, CFU numbers, antibiotic resistance profiles and relative distributions of bacterial populations. Dual-species consortia were of difficult eradication, with most antibiotics being ineffective in reducing biofilm-bacteria, particularly under low-oxygen atmospheres. Regarding microbial composition, these biofilms presented similar bacterial proportions, whereas P. aeruginosa and D. pigrum dominated the three-species consortia, with I. limosus being the smallest representative population. In general, biofilm compositions changed as a result of antibiotic treatment, with alterations being dependent on the antibiotic, concentration and oxygen condition implemented. P. aeruginosa and I. limosus dual-biofilms exhibited higher antibiotic resistance, with I. limosus persisting and occupying a significant portion together with P. aeruginosa in the overall biofilm after antibiotic treatment. Interestingly, the three-species biofilms displayed higher sensitivity, with D. pigrum and/or P. aeruginosa dominating and I. limosus populations declining in most cases. This suggests that the preponderance of D. pigrum in the biofilm was decisive to decrease I. limosus and lead to an increase in overall sensitivity of the biofilm to a large number of antibiotics. PNA FISH allowed the direct observation of the location and distribution of the three-species species within the biofilms, corroborating the dominance of D. pigrum and P. aeruginosa within the mixed-species consortia and facilitating the understanding of the real complex interactions among the bacterial species. Data highlighted that emergent species are able to establish polymicrobial consortia with common pathogens in the airways of CF patients, modulating different social activities into those communities and impacting the CF therapeutics.
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/31572
url http://hdl.handle.net/1822/31572
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Lopes, Susana Patrícia; Azevedo, N. F.; Pereira, M. O., Polymicrobial biofilms in cystic fibrosis – the role of atypical bacteria in the consortia and impact in antibiotic treatment. ICAR 2014 - III International Conference on Antimicrobial Research. Madrid, Spain, Oct. 1-3, 231-2312014.
http://www.icar-2014.org/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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