Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis

Detalhes bibliográficos
Autor(a) principal: Lopes, Susana Patrícia
Data de Publicação: 2013
Outros Autores: Azevedo, N. F., Pereira, Maria Olívia
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/28662
Resumo: A complex microbial ecosystem is present in Cystic fibrosis (CF)-airways, with uncommon species co-existing and establishing dynamic interactions with traditional pathogens. This study aimed to examine in vitro biofilm formation and susceptibility patterns of two CF-atypical bacteria, Inquilinus limosus (IL) and Dolosigranulum pigrum (DP), when associated to P. aeruginosa (PA), under oxygen-atmospheres resembling CF airways. The ability of IL and DP to form dual- and three-species biofilms with PA and to resist against antibiotics was evaluated under in vitro oxygen-restricted conditions. The fitness of biofilms was compared with monospecies consortia by individual species. Atypical species were able to develop biofilms with PA, presenting a great extent of adhesion in microtiter plate wells and achieving high cell-densities over 24h of growth. Three-species populations were well-adapted to poor-oxygen environments, showing high growth rates in these conditions. Both dual- and three-species biofilms were significantly resistant to most antibiotics, with minimum biofilm eradication concentrations (MBECs) in most cases not achievable (>1024 mg/L). Comparing with monospecies consortia, the presence of IL and DP did not disturb PA biofilms, resulting in most cases in polymicribial biofilms with increased biomass, activity and antibiotic resistance, which persisted under oxygen-restricted environments. This study evidenced the contribution of some atypical species to develop resilient polymicrobial biofilms with PA in CF-airway oxygen conditions. Hence, CF treatment will only be successful after recognizing CF-infection as polymicrobial and upon exhaustive modulation of ecological processes, which will be useful to predict the effects of new therapeutic interventions.
id RCAP_e51e272a69fd528fc8b581938413f696
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/28662
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosisCystic fibrosisBiofilmPolymicrobial infectionAntibiotic resistanceA complex microbial ecosystem is present in Cystic fibrosis (CF)-airways, with uncommon species co-existing and establishing dynamic interactions with traditional pathogens. This study aimed to examine in vitro biofilm formation and susceptibility patterns of two CF-atypical bacteria, Inquilinus limosus (IL) and Dolosigranulum pigrum (DP), when associated to P. aeruginosa (PA), under oxygen-atmospheres resembling CF airways. The ability of IL and DP to form dual- and three-species biofilms with PA and to resist against antibiotics was evaluated under in vitro oxygen-restricted conditions. The fitness of biofilms was compared with monospecies consortia by individual species. Atypical species were able to develop biofilms with PA, presenting a great extent of adhesion in microtiter plate wells and achieving high cell-densities over 24h of growth. Three-species populations were well-adapted to poor-oxygen environments, showing high growth rates in these conditions. Both dual- and three-species biofilms were significantly resistant to most antibiotics, with minimum biofilm eradication concentrations (MBECs) in most cases not achievable (>1024 mg/L). Comparing with monospecies consortia, the presence of IL and DP did not disturb PA biofilms, resulting in most cases in polymicribial biofilms with increased biomass, activity and antibiotic resistance, which persisted under oxygen-restricted environments. This study evidenced the contribution of some atypical species to develop resilient polymicrobial biofilms with PA in CF-airway oxygen conditions. Hence, CF treatment will only be successful after recognizing CF-infection as polymicrobial and upon exhaustive modulation of ecological processes, which will be useful to predict the effects of new therapeutic interventions.Universidade do MinhoLopes, Susana PatríciaAzevedo, N. F.Pereira, Maria Olívia20132013-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/28662enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T04:49:14Zoai:repositorium.sdum.uminho.pt:1822/28662Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T04:49:14Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis
title Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis
spellingShingle Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis
Lopes, Susana Patrícia
Cystic fibrosis
Biofilm
Polymicrobial infection
Antibiotic resistance
title_short Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis
title_full Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis
title_fullStr Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis
title_full_unstemmed Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis
title_sort Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis
author Lopes, Susana Patrícia
author_facet Lopes, Susana Patrícia
Azevedo, N. F.
Pereira, Maria Olívia
author_role author
author2 Azevedo, N. F.
Pereira, Maria Olívia
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Lopes, Susana Patrícia
Azevedo, N. F.
Pereira, Maria Olívia
dc.subject.por.fl_str_mv Cystic fibrosis
Biofilm
Polymicrobial infection
Antibiotic resistance
topic Cystic fibrosis
Biofilm
Polymicrobial infection
Antibiotic resistance
description A complex microbial ecosystem is present in Cystic fibrosis (CF)-airways, with uncommon species co-existing and establishing dynamic interactions with traditional pathogens. This study aimed to examine in vitro biofilm formation and susceptibility patterns of two CF-atypical bacteria, Inquilinus limosus (IL) and Dolosigranulum pigrum (DP), when associated to P. aeruginosa (PA), under oxygen-atmospheres resembling CF airways. The ability of IL and DP to form dual- and three-species biofilms with PA and to resist against antibiotics was evaluated under in vitro oxygen-restricted conditions. The fitness of biofilms was compared with monospecies consortia by individual species. Atypical species were able to develop biofilms with PA, presenting a great extent of adhesion in microtiter plate wells and achieving high cell-densities over 24h of growth. Three-species populations were well-adapted to poor-oxygen environments, showing high growth rates in these conditions. Both dual- and three-species biofilms were significantly resistant to most antibiotics, with minimum biofilm eradication concentrations (MBECs) in most cases not achievable (>1024 mg/L). Comparing with monospecies consortia, the presence of IL and DP did not disturb PA biofilms, resulting in most cases in polymicribial biofilms with increased biomass, activity and antibiotic resistance, which persisted under oxygen-restricted environments. This study evidenced the contribution of some atypical species to develop resilient polymicrobial biofilms with PA in CF-airway oxygen conditions. Hence, CF treatment will only be successful after recognizing CF-infection as polymicrobial and upon exhaustive modulation of ecological processes, which will be useful to predict the effects of new therapeutic interventions.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
dc.type.driver.fl_str_mv conference object
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/28662
url http://hdl.handle.net/1822/28662
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
_version_ 1817544429337051136