Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/28662 |
Resumo: | A complex microbial ecosystem is present in Cystic fibrosis (CF)-airways, with uncommon species co-existing and establishing dynamic interactions with traditional pathogens. This study aimed to examine in vitro biofilm formation and susceptibility patterns of two CF-atypical bacteria, Inquilinus limosus (IL) and Dolosigranulum pigrum (DP), when associated to P. aeruginosa (PA), under oxygen-atmospheres resembling CF airways. The ability of IL and DP to form dual- and three-species biofilms with PA and to resist against antibiotics was evaluated under in vitro oxygen-restricted conditions. The fitness of biofilms was compared with monospecies consortia by individual species. Atypical species were able to develop biofilms with PA, presenting a great extent of adhesion in microtiter plate wells and achieving high cell-densities over 24h of growth. Three-species populations were well-adapted to poor-oxygen environments, showing high growth rates in these conditions. Both dual- and three-species biofilms were significantly resistant to most antibiotics, with minimum biofilm eradication concentrations (MBECs) in most cases not achievable (>1024 mg/L). Comparing with monospecies consortia, the presence of IL and DP did not disturb PA biofilms, resulting in most cases in polymicribial biofilms with increased biomass, activity and antibiotic resistance, which persisted under oxygen-restricted environments. This study evidenced the contribution of some atypical species to develop resilient polymicrobial biofilms with PA in CF-airway oxygen conditions. Hence, CF treatment will only be successful after recognizing CF-infection as polymicrobial and upon exhaustive modulation of ecological processes, which will be useful to predict the effects of new therapeutic interventions. |
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Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosisCystic fibrosisBiofilmPolymicrobial infectionAntibiotic resistanceA complex microbial ecosystem is present in Cystic fibrosis (CF)-airways, with uncommon species co-existing and establishing dynamic interactions with traditional pathogens. This study aimed to examine in vitro biofilm formation and susceptibility patterns of two CF-atypical bacteria, Inquilinus limosus (IL) and Dolosigranulum pigrum (DP), when associated to P. aeruginosa (PA), under oxygen-atmospheres resembling CF airways. The ability of IL and DP to form dual- and three-species biofilms with PA and to resist against antibiotics was evaluated under in vitro oxygen-restricted conditions. The fitness of biofilms was compared with monospecies consortia by individual species. Atypical species were able to develop biofilms with PA, presenting a great extent of adhesion in microtiter plate wells and achieving high cell-densities over 24h of growth. Three-species populations were well-adapted to poor-oxygen environments, showing high growth rates in these conditions. Both dual- and three-species biofilms were significantly resistant to most antibiotics, with minimum biofilm eradication concentrations (MBECs) in most cases not achievable (>1024 mg/L). Comparing with monospecies consortia, the presence of IL and DP did not disturb PA biofilms, resulting in most cases in polymicribial biofilms with increased biomass, activity and antibiotic resistance, which persisted under oxygen-restricted environments. This study evidenced the contribution of some atypical species to develop resilient polymicrobial biofilms with PA in CF-airway oxygen conditions. Hence, CF treatment will only be successful after recognizing CF-infection as polymicrobial and upon exhaustive modulation of ecological processes, which will be useful to predict the effects of new therapeutic interventions.Universidade do MinhoLopes, Susana PatríciaAzevedo, N. F.Pereira, Maria Olívia20132013-01-01T00:00:00Zconference objectinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/1822/28662enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T04:49:14Zoai:repositorium.sdum.uminho.pt:1822/28662Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T04:49:14Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis |
title |
Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis |
spellingShingle |
Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis Lopes, Susana Patrícia Cystic fibrosis Biofilm Polymicrobial infection Antibiotic resistance |
title_short |
Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis |
title_full |
Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis |
title_fullStr |
Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis |
title_full_unstemmed |
Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis |
title_sort |
Synergistic interactions within polymicrobial biofilms formed by atypical and conventional species in cystic fibrosis |
author |
Lopes, Susana Patrícia |
author_facet |
Lopes, Susana Patrícia Azevedo, N. F. Pereira, Maria Olívia |
author_role |
author |
author2 |
Azevedo, N. F. Pereira, Maria Olívia |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Lopes, Susana Patrícia Azevedo, N. F. Pereira, Maria Olívia |
dc.subject.por.fl_str_mv |
Cystic fibrosis Biofilm Polymicrobial infection Antibiotic resistance |
topic |
Cystic fibrosis Biofilm Polymicrobial infection Antibiotic resistance |
description |
A complex microbial ecosystem is present in Cystic fibrosis (CF)-airways, with uncommon species co-existing and establishing dynamic interactions with traditional pathogens. This study aimed to examine in vitro biofilm formation and susceptibility patterns of two CF-atypical bacteria, Inquilinus limosus (IL) and Dolosigranulum pigrum (DP), when associated to P. aeruginosa (PA), under oxygen-atmospheres resembling CF airways. The ability of IL and DP to form dual- and three-species biofilms with PA and to resist against antibiotics was evaluated under in vitro oxygen-restricted conditions. The fitness of biofilms was compared with monospecies consortia by individual species. Atypical species were able to develop biofilms with PA, presenting a great extent of adhesion in microtiter plate wells and achieving high cell-densities over 24h of growth. Three-species populations were well-adapted to poor-oxygen environments, showing high growth rates in these conditions. Both dual- and three-species biofilms were significantly resistant to most antibiotics, with minimum biofilm eradication concentrations (MBECs) in most cases not achievable (>1024 mg/L). Comparing with monospecies consortia, the presence of IL and DP did not disturb PA biofilms, resulting in most cases in polymicribial biofilms with increased biomass, activity and antibiotic resistance, which persisted under oxygen-restricted environments. This study evidenced the contribution of some atypical species to develop resilient polymicrobial biofilms with PA in CF-airway oxygen conditions. Hence, CF treatment will only be successful after recognizing CF-infection as polymicrobial and upon exhaustive modulation of ecological processes, which will be useful to predict the effects of new therapeutic interventions. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2013-01-01T00:00:00Z |
dc.type.driver.fl_str_mv |
conference object |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/28662 |
url |
http://hdl.handle.net/1822/28662 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817544429337051136 |