Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms

Detalhes bibliográficos
Autor(a) principal: Queirós, Diogo
Data de Publicação: 2018
Outros Autores: Rangel, Catarina, Lemos, Paulo C., Rossetti, Simona, Serafim, Luísa Seuanes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.3390/fermentation4030058
Resumo: This work was developed within the scope of the project CICECO-Aveiro Institute of Materials (Ref. FCT UID/CTM/50011/2013) and REQUIMTE (PEst-C/EQB/LA0006/2013), financed by national funds through the FCT/MEC and when appropriate co–financed by FEDER under the PT2020 Partnership Agreement.
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spelling Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organismsFluorescence in situ Hybridization (FISH)Hardwood spent sulfite liquorMixed microbial culturesPolyhydroxyalkanoatesShort-chain organic acidsPlant ScienceFood ScienceBiochemistry, Genetics and Molecular Biology (miscellaneous)This work was developed within the scope of the project CICECO-Aveiro Institute of Materials (Ref. FCT UID/CTM/50011/2013) and REQUIMTE (PEst-C/EQB/LA0006/2013), financed by national funds through the FCT/MEC and when appropriate co–financed by FEDER under the PT2020 Partnership Agreement.The effectiveness of polyhydroxyalkanoates (PHAs) production process from a waste stream is determined by the selection of a suitable mixed microbial culture (MMC). In this work, a feedstock from the paper industry, hardwood spent sulfite liquor (HSSL), supplemented with short-chain organic acids (SCOAs) to simulate a fermented effluent, was used as substrate to enrich a MMC in PHA-storing microorganisms. A stable culture was quickly established, and during the accumulation step the selected MMC reached a maximum PHA content of 34.6% (3HB:3HV-76:24). The bacterial community was analyzed through FISH analysis. Bacteria belonging to the four main classes were identified: Betaproteobacteria (44.7 ± 2.7%), Alphaproteobacteria (13.6 ± 1.3%) and Gammaproteobacteria (2.40 ± 1.1%) and Bacteroidetes (9.20 ± 3.8%). Inside the Betaproteobacteria class, Acidovorax (71%) was the dominant genus.LAQV@REQUIMTEDQ - Departamento de QuímicaRUNQueirós, DiogoRangel, CatarinaLemos, Paulo C.Rossetti, SimonaSerafim, Luísa Seuanes2019-03-08T23:17:56Z2018-07-242018-07-24T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.3390/fermentation4030058eng2311-5637PURE: 11915797http://www.scopus.com/inward/record.url?scp=85062238618&partnerID=8YFLogxKhttps://doi.org/10.3390/fermentation4030058info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:29:39Zoai:run.unl.pt:10362/62706Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:33:47.847268Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms
title Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms
spellingShingle Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms
Queirós, Diogo
Fluorescence in situ Hybridization (FISH)
Hardwood spent sulfite liquor
Mixed microbial cultures
Polyhydroxyalkanoates
Short-chain organic acids
Plant Science
Food Science
Biochemistry, Genetics and Molecular Biology (miscellaneous)
title_short Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms
title_full Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms
title_fullStr Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms
title_full_unstemmed Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms
title_sort Impact of organic acids supplementation to hardwood spent sulfite liquor as substrate for the selection of polyhydroxyalkanoates-producing organisms
author Queirós, Diogo
author_facet Queirós, Diogo
Rangel, Catarina
Lemos, Paulo C.
Rossetti, Simona
Serafim, Luísa Seuanes
author_role author
author2 Rangel, Catarina
Lemos, Paulo C.
Rossetti, Simona
Serafim, Luísa Seuanes
author2_role author
author
author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
DQ - Departamento de Química
RUN
dc.contributor.author.fl_str_mv Queirós, Diogo
Rangel, Catarina
Lemos, Paulo C.
Rossetti, Simona
Serafim, Luísa Seuanes
dc.subject.por.fl_str_mv Fluorescence in situ Hybridization (FISH)
Hardwood spent sulfite liquor
Mixed microbial cultures
Polyhydroxyalkanoates
Short-chain organic acids
Plant Science
Food Science
Biochemistry, Genetics and Molecular Biology (miscellaneous)
topic Fluorescence in situ Hybridization (FISH)
Hardwood spent sulfite liquor
Mixed microbial cultures
Polyhydroxyalkanoates
Short-chain organic acids
Plant Science
Food Science
Biochemistry, Genetics and Molecular Biology (miscellaneous)
description This work was developed within the scope of the project CICECO-Aveiro Institute of Materials (Ref. FCT UID/CTM/50011/2013) and REQUIMTE (PEst-C/EQB/LA0006/2013), financed by national funds through the FCT/MEC and when appropriate co–financed by FEDER under the PT2020 Partnership Agreement.
publishDate 2018
dc.date.none.fl_str_mv 2018-07-24
2018-07-24T00:00:00Z
2019-03-08T23:17:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.3390/fermentation4030058
url https://doi.org/10.3390/fermentation4030058
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2311-5637
PURE: 11915797
http://www.scopus.com/inward/record.url?scp=85062238618&partnerID=8YFLogxK
https://doi.org/10.3390/fermentation4030058
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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