Pilot-scale valorisation of salmon peptone into polyhydroxyalkanoates by mixed microbial cultures under conditions of high ammonia concentration
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/154847 |
Resumo: | Funding Information: The authors are thankful for the financial support provided by the Research Council of Norway (NFR project: 267618 ) and by the FCT - Fundação para a Ciência e a Tecnologia , I.P. (Associate Laboratory Institute for Health and Bioeconomy - i4HB: LA/P/0140/2020). The authors also acknowledge João M. Carvalho, Elsa Mora, Elisabete Freitas and Mónica Centeio for assistance with chemical analysis, João R. Pereira for assistance with preliminary operation tests at pilot-scale, as well as Sverre Aarøen, Hans Richard Nyheim, Morten Andersen, and Aleksander Holmen for the production of spray dried salmon peptone. The category 2 salmon silage kindly provided by Brødrene Karlsen AS. Publisher Copyright: © 2023 The Authors |
| id |
RCAP_26a5d725124201d352eb3eeed57560a9 |
|---|---|
| oai_identifier_str |
oai:run.unl.pt:10362/154847 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Pilot-scale valorisation of salmon peptone into polyhydroxyalkanoates by mixed microbial cultures under conditions of high ammonia concentrationAcidogenic fermentationAquaculture industryBiodegradable biopolymersCulture enrichmentPHA productionProtein-rich side streamsChemical Engineering (miscellaneous)Waste Management and DisposalPollutionProcess Chemistry and TechnologySDG 6 - Clean Water and SanitationSDG 14 - Life Below WaterFunding Information: The authors are thankful for the financial support provided by the Research Council of Norway (NFR project: 267618 ) and by the FCT - Fundação para a Ciência e a Tecnologia , I.P. (Associate Laboratory Institute for Health and Bioeconomy - i4HB: LA/P/0140/2020). The authors also acknowledge João M. Carvalho, Elsa Mora, Elisabete Freitas and Mónica Centeio for assistance with chemical analysis, João R. Pereira for assistance with preliminary operation tests at pilot-scale, as well as Sverre Aarøen, Hans Richard Nyheim, Morten Andersen, and Aleksander Holmen for the production of spray dried salmon peptone. The category 2 salmon silage kindly provided by Brødrene Karlsen AS. Publisher Copyright: © 2023 The AuthorsThe aquaculture industry produces vast amounts of organic side streams that are often ensilaged for later use in low value applications. However, the potential of converting fish silage, particularly the proteinaceous fraction, into more economically valuable products such as biodegradable polyhydroxyalkanoates (PHA), has not been explored. This study investigates the valorisation of salmon peptone, the proteinaceous fraction obtained from salmon silage, into PHA by mixed microbial cultures (MMC) under conditions without nutrient limitation, where the high concentration of ammonia (up to 2.3 ± 0.2 gN/L) which the culture was subjected to has not been previously reported. Acidogenic fermentation of salmon peptone (SP) was attained in a continuous reactor, resulting in the production of a fermentate rich in acetate and butyrate (16.4 ± 1.6 gCODFP/L). The enriched MMC with PHA-accumulating organisms was selected in a sequential batch reactor (SBR) under nutrient-rich conditions (100 gCOD: 27.6 gN: 0.48 gP at OLR 7.1 gCODFP/(L.d)). The dominant microorganisms in the enriched culture were from the genus Brachymonas, which are described as capable of simultaneous cell growth and PHA accumulation, storing up to 53.0 ± 3.4% wt. of P(3HB-co-3HV) with a 3HV content of 34% wt. Despite the high ammonia concentration and constant nutrient availability, a good overall PHA yield (189 gPHA/kgSP) and global volumetric PHA productivity (3.10 ± 0.11 gPHA/(L.h)) were achieved. The results demonstrate the technological feasibility of producing PHA from salmon peptone under conditions that are typically considered unfavorable for PHA accumulation, which represents a significant novelty of this work.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNMarreiros, Bruno C.Carvalheira, MónicaHenriques, CláudiaPequito, DanielaNguyen, YenSolstad, Runar G.Eksteen, J. JohannesReis, Maria A. M.2023-07-04T22:18:02Z2023-062023-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12application/pdfhttp://hdl.handle.net/10362/154847eng2213-3437PURE: 65212432https://doi.org/10.1016/j.jece.2023.110100info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:37:18Zoai:run.unl.pt:10362/154847Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:47.499459Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Pilot-scale valorisation of salmon peptone into polyhydroxyalkanoates by mixed microbial cultures under conditions of high ammonia concentration |
| title |
Pilot-scale valorisation of salmon peptone into polyhydroxyalkanoates by mixed microbial cultures under conditions of high ammonia concentration |
| spellingShingle |
Pilot-scale valorisation of salmon peptone into polyhydroxyalkanoates by mixed microbial cultures under conditions of high ammonia concentration Marreiros, Bruno C. Acidogenic fermentation Aquaculture industry Biodegradable biopolymers Culture enrichment PHA production Protein-rich side streams Chemical Engineering (miscellaneous) Waste Management and Disposal Pollution Process Chemistry and Technology SDG 6 - Clean Water and Sanitation SDG 14 - Life Below Water |
| title_short |
Pilot-scale valorisation of salmon peptone into polyhydroxyalkanoates by mixed microbial cultures under conditions of high ammonia concentration |
| title_full |
Pilot-scale valorisation of salmon peptone into polyhydroxyalkanoates by mixed microbial cultures under conditions of high ammonia concentration |
| title_fullStr |
Pilot-scale valorisation of salmon peptone into polyhydroxyalkanoates by mixed microbial cultures under conditions of high ammonia concentration |
| title_full_unstemmed |
Pilot-scale valorisation of salmon peptone into polyhydroxyalkanoates by mixed microbial cultures under conditions of high ammonia concentration |
| title_sort |
Pilot-scale valorisation of salmon peptone into polyhydroxyalkanoates by mixed microbial cultures under conditions of high ammonia concentration |
| author |
Marreiros, Bruno C. |
| author_facet |
Marreiros, Bruno C. Carvalheira, Mónica Henriques, Cláudia Pequito, Daniela Nguyen, Yen Solstad, Runar G. Eksteen, J. Johannes Reis, Maria A. M. |
| author_role |
author |
| author2 |
Carvalheira, Mónica Henriques, Cláudia Pequito, Daniela Nguyen, Yen Solstad, Runar G. Eksteen, J. Johannes Reis, Maria A. M. |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química RUN |
| dc.contributor.author.fl_str_mv |
Marreiros, Bruno C. Carvalheira, Mónica Henriques, Cláudia Pequito, Daniela Nguyen, Yen Solstad, Runar G. Eksteen, J. Johannes Reis, Maria A. M. |
| dc.subject.por.fl_str_mv |
Acidogenic fermentation Aquaculture industry Biodegradable biopolymers Culture enrichment PHA production Protein-rich side streams Chemical Engineering (miscellaneous) Waste Management and Disposal Pollution Process Chemistry and Technology SDG 6 - Clean Water and Sanitation SDG 14 - Life Below Water |
| topic |
Acidogenic fermentation Aquaculture industry Biodegradable biopolymers Culture enrichment PHA production Protein-rich side streams Chemical Engineering (miscellaneous) Waste Management and Disposal Pollution Process Chemistry and Technology SDG 6 - Clean Water and Sanitation SDG 14 - Life Below Water |
| description |
Funding Information: The authors are thankful for the financial support provided by the Research Council of Norway (NFR project: 267618 ) and by the FCT - Fundação para a Ciência e a Tecnologia , I.P. (Associate Laboratory Institute for Health and Bioeconomy - i4HB: LA/P/0140/2020). The authors also acknowledge João M. Carvalho, Elsa Mora, Elisabete Freitas and Mónica Centeio for assistance with chemical analysis, João R. Pereira for assistance with preliminary operation tests at pilot-scale, as well as Sverre Aarøen, Hans Richard Nyheim, Morten Andersen, and Aleksander Holmen for the production of spray dried salmon peptone. The category 2 salmon silage kindly provided by Brødrene Karlsen AS. Publisher Copyright: © 2023 The Authors |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-07-04T22:18:02Z 2023-06 2023-06-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/154847 |
| url |
http://hdl.handle.net/10362/154847 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2213-3437 PURE: 65212432 https://doi.org/10.1016/j.jece.2023.110100 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
12 application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799138144398868480 |