Investigating the hepatoprotective potentiality of marine-derived steroids as promising inhibitors of liver fibrosis

Detalhes bibliográficos
Autor(a) principal: Pereira, Florbela
Data de Publicação: 2023
Outros Autores: Tammam, Mohamed A., Aly, Omnia, Sebak, Mohamed, Diab, Yasser M., Mahdy, Aldoushy, El-Demerdash, Amr
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/161172
Resumo: Mohamed A. Tammam wishes to thank Prof. E. Ioannou and Prof. V. Roussis for assistance and permission to use facilities of their laboratories at the Department of Pharmacy, National and Kapodistrian University of Athens. Mohamed A. Tammam is humbly dedicating this work to the soul of his sister Dr Mai A. Tammam who passed away on 19 of March 2022, she was always a kind supporter in all aspects of his life. Florbela Pereira gratefully acknowledges FCT – Fundação para a Ciência e a Tecnologia, I. P., for an Assistant Research Position (CEECIND/01649/2021) and the project UIDB/50006/2020 of the Associated Laboratory for Green Chemistry (LAQV) of the Network of Chemistry and Technology (REQUIMTE). Amr ElDemerdash is immensely grateful to the John Innes Centre, Norwich Research Park, United Kingdom for the post-doctoral fellowship. Amr El-Demerdash is thankful to his home university, Mansoura University, Egypt for the unlimited support, inside and outside.
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spelling Investigating the hepatoprotective potentiality of marine-derived steroids as promising inhibitors of liver fibrosisSDG 14 - Life Below WaterMohamed A. Tammam wishes to thank Prof. E. Ioannou and Prof. V. Roussis for assistance and permission to use facilities of their laboratories at the Department of Pharmacy, National and Kapodistrian University of Athens. Mohamed A. Tammam is humbly dedicating this work to the soul of his sister Dr Mai A. Tammam who passed away on 19 of March 2022, she was always a kind supporter in all aspects of his life. Florbela Pereira gratefully acknowledges FCT – Fundação para a Ciência e a Tecnologia, I. P., for an Assistant Research Position (CEECIND/01649/2021) and the project UIDB/50006/2020 of the Associated Laboratory for Green Chemistry (LAQV) of the Network of Chemistry and Technology (REQUIMTE). Amr ElDemerdash is immensely grateful to the John Innes Centre, Norwich Research Park, United Kingdom for the post-doctoral fellowship. Amr El-Demerdash is thankful to his home university, Mansoura University, Egypt for the unlimited support, inside and outside.It has been reported that organic extracts derived from soft corals belonging to the genus Sarcophyton have exhibited a wide range of therapeutic characteristics. Based on biochemical and histological techniques, we aimed to assess the hepatoprotective role of the organic extract and its principal steroidal contents derived from the Red Sea soft coral Sarcophyton glaucum on acetaminophen-induced liver fibrosis in rats. Serum liver function parameters (ALT, AST, ALP and total bilirubin) were quantified using a spectrophotometer, and both alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) levels were determined by using enzyme-linked immunosorbent assay (ELISA) kits while transformed growth factor beta (TGF-β) and tumor necrosis factor α (TNF-α) in liver tissue homogenate were determined using ELISA, and TGF-β and TNF-α gene expression in liver tissue was determined using real-time PCR following extraction and purification. Histopathological alterations in hepatic tissue were also examined under a microscope. In order to prioritize the isolation and characterization of the most promising marine steroids from the organic extract of the Red Sea soft coral Sarcophyton glaucum as hepatoprotective agents, a computational approach was employed. This approach involved molecular docking (MDock) and analysis of the structure–activity relationship (SAR) against glutathione-S-transferase (GST) and Cu–Zn human superoxide dismutase (Cu–ZnSOD) enzymes. Although the major role in the detoxification of foreign chemicals and toxic metabolites of GST and SOD enzymes is known, there is a lack of knowledge about the mode of action of the hepatoprotective process and those of the targets involved. The present study investigated the multiple interactions of a series of marine steroids with the GST and SOD enzymes, in order to reveal insights into the process of hepatoprotection.DQ - Departamento de QuímicaLAQV@REQUIMTERUNPereira, FlorbelaTammam, Mohamed A.Aly, OmniaSebak, MohamedDiab, Yasser M.Mahdy, AldoushyEl-Demerdash, Amr2023-12-12T22:51:46Z2023-09-132023-09-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14application/pdfhttp://hdl.handle.net/10362/161172eng2046-2069PURE: 78201930https://doi.org/10.1039/D3RA04843Hinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:44:00Zoai:run.unl.pt:10362/161172Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:58:24.265533Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Investigating the hepatoprotective potentiality of marine-derived steroids as promising inhibitors of liver fibrosis
title Investigating the hepatoprotective potentiality of marine-derived steroids as promising inhibitors of liver fibrosis
spellingShingle Investigating the hepatoprotective potentiality of marine-derived steroids as promising inhibitors of liver fibrosis
Pereira, Florbela
SDG 14 - Life Below Water
title_short Investigating the hepatoprotective potentiality of marine-derived steroids as promising inhibitors of liver fibrosis
title_full Investigating the hepatoprotective potentiality of marine-derived steroids as promising inhibitors of liver fibrosis
title_fullStr Investigating the hepatoprotective potentiality of marine-derived steroids as promising inhibitors of liver fibrosis
title_full_unstemmed Investigating the hepatoprotective potentiality of marine-derived steroids as promising inhibitors of liver fibrosis
title_sort Investigating the hepatoprotective potentiality of marine-derived steroids as promising inhibitors of liver fibrosis
author Pereira, Florbela
author_facet Pereira, Florbela
Tammam, Mohamed A.
Aly, Omnia
Sebak, Mohamed
Diab, Yasser M.
Mahdy, Aldoushy
El-Demerdash, Amr
author_role author
author2 Tammam, Mohamed A.
Aly, Omnia
Sebak, Mohamed
Diab, Yasser M.
Mahdy, Aldoushy
El-Demerdash, Amr
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
LAQV@REQUIMTE
RUN
dc.contributor.author.fl_str_mv Pereira, Florbela
Tammam, Mohamed A.
Aly, Omnia
Sebak, Mohamed
Diab, Yasser M.
Mahdy, Aldoushy
El-Demerdash, Amr
dc.subject.por.fl_str_mv SDG 14 - Life Below Water
topic SDG 14 - Life Below Water
description Mohamed A. Tammam wishes to thank Prof. E. Ioannou and Prof. V. Roussis for assistance and permission to use facilities of their laboratories at the Department of Pharmacy, National and Kapodistrian University of Athens. Mohamed A. Tammam is humbly dedicating this work to the soul of his sister Dr Mai A. Tammam who passed away on 19 of March 2022, she was always a kind supporter in all aspects of his life. Florbela Pereira gratefully acknowledges FCT – Fundação para a Ciência e a Tecnologia, I. P., for an Assistant Research Position (CEECIND/01649/2021) and the project UIDB/50006/2020 of the Associated Laboratory for Green Chemistry (LAQV) of the Network of Chemistry and Technology (REQUIMTE). Amr ElDemerdash is immensely grateful to the John Innes Centre, Norwich Research Park, United Kingdom for the post-doctoral fellowship. Amr El-Demerdash is thankful to his home university, Mansoura University, Egypt for the unlimited support, inside and outside.
publishDate 2023
dc.date.none.fl_str_mv 2023-12-12T22:51:46Z
2023-09-13
2023-09-13T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/161172
url http://hdl.handle.net/10362/161172
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2046-2069
PURE: 78201930
https://doi.org/10.1039/D3RA04843H
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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