Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells

Detalhes bibliográficos
Autor(a) principal: Araújo, Diana
Data de Publicação: 2023
Outros Autores: Rodrigues, Thomas, Roma-Rodrigues, Catarina, Alves, Vitor D., Fernandes, Alexandra R., Freitas, Filomena
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/30243
Resumo: Chitin-glucan complex (CGC) hydrogels were fabricated by coagulation of the biopolymer from an aqueous alkaline solution, and their morphology, swelling behavior, mechanical, rheological, and biological properties were studied. In addition, their in vitro drug loading/release ability and permeation through mimic-skin artificial membranes (Strat-M) were assessed. The CGC hydrogels prepared from 4 and 6 wt% CGC suspensions (Na51*4 and Na51*6 hydrogels, respectively) had polymer contents of 2.40 0.15 and 3.09 0.22 wt%, respectively, and displayed a highly porous microstructure, characterized by compressive moduli of 39.36 and 47.30 kPa and storage moduli of 523.20 and 7012.25 Pa, respectively. Both hydrogels had a spontaneous and almost immediate swelling in aqueous media, and a high-water retention capacity (>80%), after 30 min incubation at 37 C. Nevertheless, the Na51*4 hydrogels had higher fatigue resistance and slightly higher-water retention capacity. These hydrogels were loaded with caffeine, ibuprofen, diclofenac, or salicylic acid, reaching entrapment efficiency values ranging between 13.11 0.49% for caffeine, and 15.15 1.54% for salicylic acid. Similar release profiles in PBS were observed for all tested APIs, comprising an initial fast release followed by a steady slower release. In vitro permeation experiments through Strat-M membranes using Franz diffusion cells showed considerably higher permeation fluxes for caffeine (33.09 g/cm2/h) and salicylic acid (19.53 g/cm2/h), compared to ibuprofen sodium and diclofenac sodium (4.26 and 0.44 g/cm2/h, respectively). Analysis in normal human dermal fibroblasts revealed that CGC hydrogels have no major effects on the viability, migration ability, and morphology of the cells. Given their demonstrated features, CGC hydrogels are very promising structures, displaying tunable physical properties, which support their future development into novel transdermal drug delivery platforms.
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spelling Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cellshydrogelschitin-glucan complexpolymer concentrationdrug deliveryFranz diffusion cellpermeation studiesChitin-glucan complex (CGC) hydrogels were fabricated by coagulation of the biopolymer from an aqueous alkaline solution, and their morphology, swelling behavior, mechanical, rheological, and biological properties were studied. In addition, their in vitro drug loading/release ability and permeation through mimic-skin artificial membranes (Strat-M) were assessed. The CGC hydrogels prepared from 4 and 6 wt% CGC suspensions (Na51*4 and Na51*6 hydrogels, respectively) had polymer contents of 2.40 0.15 and 3.09 0.22 wt%, respectively, and displayed a highly porous microstructure, characterized by compressive moduli of 39.36 and 47.30 kPa and storage moduli of 523.20 and 7012.25 Pa, respectively. Both hydrogels had a spontaneous and almost immediate swelling in aqueous media, and a high-water retention capacity (>80%), after 30 min incubation at 37 C. Nevertheless, the Na51*4 hydrogels had higher fatigue resistance and slightly higher-water retention capacity. These hydrogels were loaded with caffeine, ibuprofen, diclofenac, or salicylic acid, reaching entrapment efficiency values ranging between 13.11 0.49% for caffeine, and 15.15 1.54% for salicylic acid. Similar release profiles in PBS were observed for all tested APIs, comprising an initial fast release followed by a steady slower release. In vitro permeation experiments through Strat-M membranes using Franz diffusion cells showed considerably higher permeation fluxes for caffeine (33.09 g/cm2/h) and salicylic acid (19.53 g/cm2/h), compared to ibuprofen sodium and diclofenac sodium (4.26 and 0.44 g/cm2/h, respectively). Analysis in normal human dermal fibroblasts revealed that CGC hydrogels have no major effects on the viability, migration ability, and morphology of the cells. Given their demonstrated features, CGC hydrogels are very promising structures, displaying tunable physical properties, which support their future development into novel transdermal drug delivery platforms.MDPIRepositório da Universidade de LisboaAraújo, DianaRodrigues, ThomasRoma-Rodrigues, CatarinaAlves, Vitor D.Fernandes, Alexandra R.Freitas, Filomena2024-02-29T12:11:21Z2023-022023-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/30243engAraújo, D.; Rodrigues, T.; Roma-Rodrigues, C.; Alves, V.D.; Fernandes, A.R.; Freitas, F. Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells. Polymers 2023, 15, 79110.3390/ polym15040791info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-03T01:32:45Zoai:www.repository.utl.pt:10400.5/30243Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:12:16.352834Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells
title Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells
spellingShingle Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells
Araújo, Diana
hydrogels
chitin-glucan complex
polymer concentration
drug delivery
Franz diffusion cell
permeation studies
title_short Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells
title_full Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells
title_fullStr Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells
title_full_unstemmed Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells
title_sort Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells
author Araújo, Diana
author_facet Araújo, Diana
Rodrigues, Thomas
Roma-Rodrigues, Catarina
Alves, Vitor D.
Fernandes, Alexandra R.
Freitas, Filomena
author_role author
author2 Rodrigues, Thomas
Roma-Rodrigues, Catarina
Alves, Vitor D.
Fernandes, Alexandra R.
Freitas, Filomena
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Araújo, Diana
Rodrigues, Thomas
Roma-Rodrigues, Catarina
Alves, Vitor D.
Fernandes, Alexandra R.
Freitas, Filomena
dc.subject.por.fl_str_mv hydrogels
chitin-glucan complex
polymer concentration
drug delivery
Franz diffusion cell
permeation studies
topic hydrogels
chitin-glucan complex
polymer concentration
drug delivery
Franz diffusion cell
permeation studies
description Chitin-glucan complex (CGC) hydrogels were fabricated by coagulation of the biopolymer from an aqueous alkaline solution, and their morphology, swelling behavior, mechanical, rheological, and biological properties were studied. In addition, their in vitro drug loading/release ability and permeation through mimic-skin artificial membranes (Strat-M) were assessed. The CGC hydrogels prepared from 4 and 6 wt% CGC suspensions (Na51*4 and Na51*6 hydrogels, respectively) had polymer contents of 2.40 0.15 and 3.09 0.22 wt%, respectively, and displayed a highly porous microstructure, characterized by compressive moduli of 39.36 and 47.30 kPa and storage moduli of 523.20 and 7012.25 Pa, respectively. Both hydrogels had a spontaneous and almost immediate swelling in aqueous media, and a high-water retention capacity (>80%), after 30 min incubation at 37 C. Nevertheless, the Na51*4 hydrogels had higher fatigue resistance and slightly higher-water retention capacity. These hydrogels were loaded with caffeine, ibuprofen, diclofenac, or salicylic acid, reaching entrapment efficiency values ranging between 13.11 0.49% for caffeine, and 15.15 1.54% for salicylic acid. Similar release profiles in PBS were observed for all tested APIs, comprising an initial fast release followed by a steady slower release. In vitro permeation experiments through Strat-M membranes using Franz diffusion cells showed considerably higher permeation fluxes for caffeine (33.09 g/cm2/h) and salicylic acid (19.53 g/cm2/h), compared to ibuprofen sodium and diclofenac sodium (4.26 and 0.44 g/cm2/h, respectively). Analysis in normal human dermal fibroblasts revealed that CGC hydrogels have no major effects on the viability, migration ability, and morphology of the cells. Given their demonstrated features, CGC hydrogels are very promising structures, displaying tunable physical properties, which support their future development into novel transdermal drug delivery platforms.
publishDate 2023
dc.date.none.fl_str_mv 2023-02
2023-02-01T00:00:00Z
2024-02-29T12:11:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/30243
url http://hdl.handle.net/10400.5/30243
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Araújo, D.; Rodrigues, T.; Roma-Rodrigues, C.; Alves, V.D.; Fernandes, A.R.; Freitas, F. Chitin-Glucan complex hydrogels: physical-chemical characterization, stability, in vitro drug permeation, and biological assessment in primary cells. Polymers 2023, 15, 791
10.3390/ polym15040791
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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