Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessment
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/22101 |
Resumo: | Chitin-glucan complex (CGC) was used for the first time for the preparation of hydrogels. Alkali solvent systems, NaOH and KOH solutions, either at 1 or 5 mol/L, were used for CGC dissolution using a freeze-thaw procedure (freezing at −20 °C and thawing at room temperature; four cycles). The CGC solutions thus obtained were subjected to dialysis that induced the spontaneous gelation of the biopolymer, yielding translucid hydrogels with a yellowish coloration. Although all CGC hydrogels exhibited porous microstructures, high water content (above 97%) and good mechanical properties, their morphology, viscoelastic properties and texture were influenced by the type of solvent system used for CGC dissolution, as well as by their ionic strength. The K-based hydrogels presented a less compact network with larger pores and exhibited lower elastic properties. The Na-based hydrogels, on the other hand, exhibited a denser structure with smaller pores and a stiffer gel structure. These results show that it is possible to prepare CGC hydrogels with differing characteristics that can be suitable for different applications. Furthermore, all hydrogels were non-cytotoxic towards L929 fibroblasts and HaCaT keratinocytes. This study demonstrates CGC can be used to prepare biocompatible hydrogels with properties render them promising biomaterials |
id |
RCAP_4f6753083d0f0373400f18f86b39ea83 |
---|---|
oai_identifier_str |
oai:www.repository.utl.pt:10400.5/22101 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessmentchitin-glucan complex (CGC)hydrogelsbiocompatibilityChitin-glucan complex (CGC) was used for the first time for the preparation of hydrogels. Alkali solvent systems, NaOH and KOH solutions, either at 1 or 5 mol/L, were used for CGC dissolution using a freeze-thaw procedure (freezing at −20 °C and thawing at room temperature; four cycles). The CGC solutions thus obtained were subjected to dialysis that induced the spontaneous gelation of the biopolymer, yielding translucid hydrogels with a yellowish coloration. Although all CGC hydrogels exhibited porous microstructures, high water content (above 97%) and good mechanical properties, their morphology, viscoelastic properties and texture were influenced by the type of solvent system used for CGC dissolution, as well as by their ionic strength. The K-based hydrogels presented a less compact network with larger pores and exhibited lower elastic properties. The Na-based hydrogels, on the other hand, exhibited a denser structure with smaller pores and a stiffer gel structure. These results show that it is possible to prepare CGC hydrogels with differing characteristics that can be suitable for different applications. Furthermore, all hydrogels were non-cytotoxic towards L929 fibroblasts and HaCaT keratinocytes. This study demonstrates CGC can be used to prepare biocompatible hydrogels with properties render them promising biomaterialsElsevierRepositório da Universidade de LisboaAraújo, DianaDelgado Alves, VitorLima, Sofia A.C.Reis, SaletteFreitas, FilomenaReis, Maria A.M.2021-09-29T13:10:40Z20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22101engInternational Journal of Biological Macromolecules Volume 156, 1 August 2020, Pages 1104-1111https://doi.org/10.1016/j.ijbiomac.2019.11.141info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:40Zoai:www.repository.utl.pt:10400.5/22101Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:36.120436Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessment |
title |
Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessment |
spellingShingle |
Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessment Araújo, Diana chitin-glucan complex (CGC) hydrogels biocompatibility |
title_short |
Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessment |
title_full |
Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessment |
title_fullStr |
Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessment |
title_full_unstemmed |
Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessment |
title_sort |
Novel hydrogels based on yeast chitin-glucan complex: Characterization and safety assessment |
author |
Araújo, Diana |
author_facet |
Araújo, Diana Delgado Alves, Vitor Lima, Sofia A.C. Reis, Salette Freitas, Filomena Reis, Maria A.M. |
author_role |
author |
author2 |
Delgado Alves, Vitor Lima, Sofia A.C. Reis, Salette Freitas, Filomena Reis, Maria A.M. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Araújo, Diana Delgado Alves, Vitor Lima, Sofia A.C. Reis, Salette Freitas, Filomena Reis, Maria A.M. |
dc.subject.por.fl_str_mv |
chitin-glucan complex (CGC) hydrogels biocompatibility |
topic |
chitin-glucan complex (CGC) hydrogels biocompatibility |
description |
Chitin-glucan complex (CGC) was used for the first time for the preparation of hydrogels. Alkali solvent systems, NaOH and KOH solutions, either at 1 or 5 mol/L, were used for CGC dissolution using a freeze-thaw procedure (freezing at −20 °C and thawing at room temperature; four cycles). The CGC solutions thus obtained were subjected to dialysis that induced the spontaneous gelation of the biopolymer, yielding translucid hydrogels with a yellowish coloration. Although all CGC hydrogels exhibited porous microstructures, high water content (above 97%) and good mechanical properties, their morphology, viscoelastic properties and texture were influenced by the type of solvent system used for CGC dissolution, as well as by their ionic strength. The K-based hydrogels presented a less compact network with larger pores and exhibited lower elastic properties. The Na-based hydrogels, on the other hand, exhibited a denser structure with smaller pores and a stiffer gel structure. These results show that it is possible to prepare CGC hydrogels with differing characteristics that can be suitable for different applications. Furthermore, all hydrogels were non-cytotoxic towards L929 fibroblasts and HaCaT keratinocytes. This study demonstrates CGC can be used to prepare biocompatible hydrogels with properties render them promising biomaterials |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z 2021-09-29T13:10:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/22101 |
url |
http://hdl.handle.net/10400.5/22101 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Biological Macromolecules Volume 156, 1 August 2020, Pages 1104-1111 https://doi.org/10.1016/j.ijbiomac.2019.11.141 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131158640852992 |