Long-chain fatty acids degradation by desulfomonile species and proposal of candidatus Desulfomonile palmitatoxidans
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/71263 |
Resumo: | The datasets generated for this study can be found in the European Nucleotide Archive (ENA)–LS453291 (https://www.ebi.ac.uk/ena/browser/view/LS453291), PRJEB26656 (https://www.ebi.ac.uk/ena/browser/view/PRJEB26656), PRJEB35900 (https://www.ebi.ac.uk/ena/browser/view/PRJEB35900). |
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Long-chain fatty acids degradation by desulfomonile species and proposal of candidatus Desulfomonile palmitatoxidansDesulfomonilepalmitatesulfate reductionlong-chain fatty acidsorganohalide respirationmetagenomeScience & TechnologyThe datasets generated for this study can be found in the European Nucleotide Archive (ENA)–LS453291 (https://www.ebi.ac.uk/ena/browser/view/LS453291), PRJEB26656 (https://www.ebi.ac.uk/ena/browser/view/PRJEB26656), PRJEB35900 (https://www.ebi.ac.uk/ena/browser/view/PRJEB35900).Microbial communities with the ability to convert long-chain fatty acids (LCFA) coupled to sulfate reduction can be important in the removal of these compounds from wastewater. In this work, an enrichment culture, able to oxidize the long-chain fatty acid palmitate (C16:0) coupled to sulfate reduction, was obtained from anaerobic granular sludge. Microscopic analysis of this culture, designated HP culture, revealed that it was mainly composed of one morphotype with a typical collar-like cell wall invagination, a distinct morphological feature of the Desulfomonile genus. 16S rRNA gene amplicon and metagenome-assembled genome (MAG) indeed confirmed that the abundant phylotype in HP culture belong to Desulfomonile genus [ca. 92% 16S rRNA gene sequences closely related to Desulfomonile spp.; and ca. 82% whole genome shotgun (WGS)]. Based on similar cell morphology and average nucleotide identity (ANI) (77%) between the Desulfomonile sp. in HP culture and the type strain Desulfomonile tiedjei strain DCB-1T, we propose a novel species designated as Candidatus Desulfomonile palmitatoxidans. This bacterium shares 94.3 and 93.6% 16S rRNA gene identity with Desulfomonile limimaris strain DCB-MT and D. tiedjei strain DCB-1T, respectively. Based on sequence abundance of Desulfomonile-morphotype in HP culture, its predominance in the microscopic observations, and presence of several genes coding for enzymes involved in LCFA degradation, the proposed species Ca. Desulfomonile palmitatoxidans most probably plays an important role in palmitate degradation in HP culture. Analysis of the growth of HP culture and D. tiedjei strain DCB-1T with short- (butyrate), medium- (caprylate) and long-chain fatty acids (palmitate, stearate, and oleate) showed that both cultures degraded all fatty acids coupled to sulfate reduction, except oleate that was only utilized by HP culture. In the absence of sulfate, neither HP culture, nor D. tiedjei strain DCB-1T degraded palmitate when incubated with Methanobacterium formicicum as a possible methanogenic syntrophic partner. Unlike D. tiedjei strain DCB-1T, Ca. Desulfomonile palmitatoxidans lacks reductive dehalogenase genes in its genome, and HP culture was not able to grow by organohalide respiration. An emended description of the genus Desulfomonile is proposed. Our study reveals an unrecognized LCFA degradation feature of the Desulfomonile genus.This study was funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of project MORE (PTDC/AAG-TEC/3500/2014; POCI-01-0145-FEDER016575), and of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020–Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionFrontiers Media S.A.Universidade do MinhoAlves, J. I.Salvador, Andreia Filipa FerreiraCarvalho, Ana Rita CastroZheng, YingNijsse, BartAtashgahi, SiavashSousa, Diana Zita MachadoStams, Alfons Johannes MariaAlves, M. M.Cavaleiro, Ana Júlia20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/71263engAlves, J. I.; Salvador, Andreia F.; Castro, Rita; Zheng, Ying; Nijsse, Bart; Atashgahi, Siavash; Sousa, Diana Z.; Stams, A. J. M.; Alves, M. Madalena; Cavaleiro, Ana Júlia, Long-chain fatty acids degradation by desulfomonile species and proposal of candidatus Desulfomonile palmitatoxidans. Frontiers in Microbiology, 11(539604), 20201664-302X10.3389/fmicb.2020.539604539604http://journal.frontiersin.org/journal/microbiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:31:37Zoai:repositorium.sdum.uminho.pt:1822/71263Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:31:37Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Long-chain fatty acids degradation by desulfomonile species and proposal of candidatus Desulfomonile palmitatoxidans |
| title |
Long-chain fatty acids degradation by desulfomonile species and proposal of candidatus Desulfomonile palmitatoxidans |
| spellingShingle |
Long-chain fatty acids degradation by desulfomonile species and proposal of candidatus Desulfomonile palmitatoxidans Alves, J. I. Desulfomonile palmitate sulfate reduction long-chain fatty acids organohalide respiration metagenome Science & Technology |
| title_short |
Long-chain fatty acids degradation by desulfomonile species and proposal of candidatus Desulfomonile palmitatoxidans |
| title_full |
Long-chain fatty acids degradation by desulfomonile species and proposal of candidatus Desulfomonile palmitatoxidans |
| title_fullStr |
Long-chain fatty acids degradation by desulfomonile species and proposal of candidatus Desulfomonile palmitatoxidans |
| title_full_unstemmed |
Long-chain fatty acids degradation by desulfomonile species and proposal of candidatus Desulfomonile palmitatoxidans |
| title_sort |
Long-chain fatty acids degradation by desulfomonile species and proposal of candidatus Desulfomonile palmitatoxidans |
| author |
Alves, J. I. |
| author_facet |
Alves, J. I. Salvador, Andreia Filipa Ferreira Carvalho, Ana Rita Castro Zheng, Ying Nijsse, Bart Atashgahi, Siavash Sousa, Diana Zita Machado Stams, Alfons Johannes Maria Alves, M. M. Cavaleiro, Ana Júlia |
| author_role |
author |
| author2 |
Salvador, Andreia Filipa Ferreira Carvalho, Ana Rita Castro Zheng, Ying Nijsse, Bart Atashgahi, Siavash Sousa, Diana Zita Machado Stams, Alfons Johannes Maria Alves, M. M. Cavaleiro, Ana Júlia |
| author2_role |
author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Alves, J. I. Salvador, Andreia Filipa Ferreira Carvalho, Ana Rita Castro Zheng, Ying Nijsse, Bart Atashgahi, Siavash Sousa, Diana Zita Machado Stams, Alfons Johannes Maria Alves, M. M. Cavaleiro, Ana Júlia |
| dc.subject.por.fl_str_mv |
Desulfomonile palmitate sulfate reduction long-chain fatty acids organohalide respiration metagenome Science & Technology |
| topic |
Desulfomonile palmitate sulfate reduction long-chain fatty acids organohalide respiration metagenome Science & Technology |
| description |
The datasets generated for this study can be found in the European Nucleotide Archive (ENA)–LS453291 (https://www.ebi.ac.uk/ena/browser/view/LS453291), PRJEB26656 (https://www.ebi.ac.uk/ena/browser/view/PRJEB26656), PRJEB35900 (https://www.ebi.ac.uk/ena/browser/view/PRJEB35900). |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 2020-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/71263 |
| url |
http://hdl.handle.net/1822/71263 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Alves, J. I.; Salvador, Andreia F.; Castro, Rita; Zheng, Ying; Nijsse, Bart; Atashgahi, Siavash; Sousa, Diana Z.; Stams, A. J. M.; Alves, M. Madalena; Cavaleiro, Ana Júlia, Long-chain fatty acids degradation by desulfomonile species and proposal of candidatus Desulfomonile palmitatoxidans. Frontiers in Microbiology, 11(539604), 2020 1664-302X 10.3389/fmicb.2020.539604 539604 http://journal.frontiersin.org/journal/microbiology |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Frontiers Media S.A. |
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Frontiers Media S.A. |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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mluisa.alvim@gmail.com |
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1817544651471585280 |