Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions

Detalhes bibliográficos
Autor(a) principal: Janek, Tomasz
Data de Publicação: 2016
Outros Autores: Rodrigues, L. R., Gudiña, Eduardo J., Czyznikowska, Zaneta
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/42260
Resumo: "Available online 28 June 2016"
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spelling Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ionsLipopeptidesPseudofactin IIMetal-ion interactionCircular dichroismMolecular modellingUropathogenic microorganismsScience & Technology"Available online 28 June 2016"The interaction of natural lipopeptide pseudofactin II with a series of doubly charged metal cations was examined by matrixassisted laserdesorption ionizationtime of flight (MALDI-TOF) mass spectrometry and molecular modelling. The molecular modelling for metal-pseudofactin II provides information on the metalpeptide binding sites. Overall, Mg2+, Ca2+ and Zn2+ favor the association with oxygen atoms spanning the peptide backbone, whereas Cu2+ is coordinated by three nitrogens. Circular dichroism (CD) results confirmed that Zn2+ and Cu2+ can disrupt the secondary structure of pseudofactin II at high concentrations, while Ca2+ and Mg2+ did not essentially affect the structure of the lipopeptide. Interestingly, our results showed that the addition of Zn2+ and Cu2+ helped smaller micelles to form larger micellar aggregates. Since pseudofactin II binds metals, we tested whether this phenomena was somehow related to its antimicrobial activity against Staphylococcus epidermidis and Proteus mirabilis. We found that the antimicrobial effect of pseudofactin II was increased by supplementation of culture media with all tested divalent metal ions. Finally, by using Gram-positive and Gram-negative bacteria we showed that the higher antimicrobial activity of metal complexes of pseudofactin II is attributed to the disruption of the cytoplasmic membrane.Fundação para a Ciência e Tecnologia (FCT) for the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684)ElsevierUniversidade do MinhoJanek, TomaszRodrigues, L. R.Gudiña, Eduardo J.Czyznikowska, Zaneta20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/42260engJanek, Tomasz; Rodrigues, Lígia R.; Gudiña, Eduardo J.; Czyznikowska, Zaneta, Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions. Colloids and Surfaces B: Biointerfaces, 146, 498-506, 20160927-77650927-776510.1016/j.colsurfb.2016.06.05527416562http://www.journals.elsevier.com/colloids-and-surfaces-b-biointerfaces/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:30Zoai:repositorium.sdum.uminho.pt:1822/42260Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:25.203781Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions
title Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions
spellingShingle Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions
Janek, Tomasz
Lipopeptides
Pseudofactin II
Metal-ion interaction
Circular dichroism
Molecular modelling
Uropathogenic microorganisms
Science & Technology
title_short Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions
title_full Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions
title_fullStr Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions
title_full_unstemmed Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions
title_sort Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions
author Janek, Tomasz
author_facet Janek, Tomasz
Rodrigues, L. R.
Gudiña, Eduardo J.
Czyznikowska, Zaneta
author_role author
author2 Rodrigues, L. R.
Gudiña, Eduardo J.
Czyznikowska, Zaneta
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Janek, Tomasz
Rodrigues, L. R.
Gudiña, Eduardo J.
Czyznikowska, Zaneta
dc.subject.por.fl_str_mv Lipopeptides
Pseudofactin II
Metal-ion interaction
Circular dichroism
Molecular modelling
Uropathogenic microorganisms
Science & Technology
topic Lipopeptides
Pseudofactin II
Metal-ion interaction
Circular dichroism
Molecular modelling
Uropathogenic microorganisms
Science & Technology
description "Available online 28 June 2016"
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/42260
url http://hdl.handle.net/1822/42260
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Janek, Tomasz; Rodrigues, Lígia R.; Gudiña, Eduardo J.; Czyznikowska, Zaneta, Structure and mode of action of cyclic lipopeptide pseudofactin II with divalent metal ions. Colloids and Surfaces B: Biointerfaces, 146, 498-506, 2016
0927-7765
0927-7765
10.1016/j.colsurfb.2016.06.055
27416562
http://www.journals.elsevier.com/colloids-and-surfaces-b-biointerfaces/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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