INFOGEST static in vitro simulation of gastrointestinal food digestion
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/6441 |
Resumo: | Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities. |
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INFOGEST static in vitro simulation of gastrointestinal food digestionAmino AcidsBileBiomimetic MaterialsDigestionEatingEnzyme AssaysFatty AcidsFoodFood IngredientsGastric JuiceHumansHydrogen-Ion ConcentrationHydrolysisIntestinesMouthOligosaccharidesPeptide FragmentsSalivaStomachModels, BiologicalAvaliação do Impacte em SaúdeComposição dos AlimentosDeveloping a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.Nature ResearchRepositório Científico do Instituto Nacional de SaúdeBrodkorb, AndréEgger, LottiAlminger, MarieAlvito, PaulaAssunção, RicardoBallance, SimonBohn, TorstenBourlieu-Lacanal, ClaireBoutrou, RachelCarrière, FrédéricClemente, AlfonsoCorredig, MilenaDupont, DidierDufour, ClaireEdwards, CathrinaGolding, MattKarakaya, SibelKirkhus, BenteLe Feunteun, StevenLesmes, UriMacierzanka, AdamMackie, Alan R.Martins, CarlaMarze, SébastienMcClements, David JulianMénard, OliviaMinekus, MansPortmann, RetoSantos, Cláudia N.Souchon, IsabelleSingh, R. PaulVegarud, Gerd E.Wickham, Martin S.J.Weitschies, WernerRecio, Isidra2019-07-10T14:16:19Z2019-042019-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/10400.18/6441engNat Protoc. 2019 Apr;14(4):991-1014. doi: 10.1038/s41596-018-0119-1. Epub 2019 Mar 181754-218910.1038/s41596-018-0119-1info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:41:24Zoai:repositorio.insa.pt:10400.18/6441Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:41:04.124727Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
INFOGEST static in vitro simulation of gastrointestinal food digestion |
title |
INFOGEST static in vitro simulation of gastrointestinal food digestion |
spellingShingle |
INFOGEST static in vitro simulation of gastrointestinal food digestion Brodkorb, André Amino Acids Bile Biomimetic Materials Digestion Eating Enzyme Assays Fatty Acids Food Food Ingredients Gastric Juice Humans Hydrogen-Ion Concentration Hydrolysis Intestines Mouth Oligosaccharides Peptide Fragments Saliva Stomach Models, Biological Avaliação do Impacte em Saúde Composição dos Alimentos |
title_short |
INFOGEST static in vitro simulation of gastrointestinal food digestion |
title_full |
INFOGEST static in vitro simulation of gastrointestinal food digestion |
title_fullStr |
INFOGEST static in vitro simulation of gastrointestinal food digestion |
title_full_unstemmed |
INFOGEST static in vitro simulation of gastrointestinal food digestion |
title_sort |
INFOGEST static in vitro simulation of gastrointestinal food digestion |
author |
Brodkorb, André |
author_facet |
Brodkorb, André Egger, Lotti Alminger, Marie Alvito, Paula Assunção, Ricardo Ballance, Simon Bohn, Torsten Bourlieu-Lacanal, Claire Boutrou, Rachel Carrière, Frédéric Clemente, Alfonso Corredig, Milena Dupont, Didier Dufour, Claire Edwards, Cathrina Golding, Matt Karakaya, Sibel Kirkhus, Bente Le Feunteun, Steven Lesmes, Uri Macierzanka, Adam Mackie, Alan R. Martins, Carla Marze, Sébastien McClements, David Julian Ménard, Olivia Minekus, Mans Portmann, Reto Santos, Cláudia N. Souchon, Isabelle Singh, R. Paul Vegarud, Gerd E. Wickham, Martin S.J. Weitschies, Werner Recio, Isidra |
author_role |
author |
author2 |
Egger, Lotti Alminger, Marie Alvito, Paula Assunção, Ricardo Ballance, Simon Bohn, Torsten Bourlieu-Lacanal, Claire Boutrou, Rachel Carrière, Frédéric Clemente, Alfonso Corredig, Milena Dupont, Didier Dufour, Claire Edwards, Cathrina Golding, Matt Karakaya, Sibel Kirkhus, Bente Le Feunteun, Steven Lesmes, Uri Macierzanka, Adam Mackie, Alan R. Martins, Carla Marze, Sébastien McClements, David Julian Ménard, Olivia Minekus, Mans Portmann, Reto Santos, Cláudia N. Souchon, Isabelle Singh, R. Paul Vegarud, Gerd E. Wickham, Martin S.J. Weitschies, Werner Recio, Isidra |
author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Brodkorb, André Egger, Lotti Alminger, Marie Alvito, Paula Assunção, Ricardo Ballance, Simon Bohn, Torsten Bourlieu-Lacanal, Claire Boutrou, Rachel Carrière, Frédéric Clemente, Alfonso Corredig, Milena Dupont, Didier Dufour, Claire Edwards, Cathrina Golding, Matt Karakaya, Sibel Kirkhus, Bente Le Feunteun, Steven Lesmes, Uri Macierzanka, Adam Mackie, Alan R. Martins, Carla Marze, Sébastien McClements, David Julian Ménard, Olivia Minekus, Mans Portmann, Reto Santos, Cláudia N. Souchon, Isabelle Singh, R. Paul Vegarud, Gerd E. Wickham, Martin S.J. Weitschies, Werner Recio, Isidra |
dc.subject.por.fl_str_mv |
Amino Acids Bile Biomimetic Materials Digestion Eating Enzyme Assays Fatty Acids Food Food Ingredients Gastric Juice Humans Hydrogen-Ion Concentration Hydrolysis Intestines Mouth Oligosaccharides Peptide Fragments Saliva Stomach Models, Biological Avaliação do Impacte em Saúde Composição dos Alimentos |
topic |
Amino Acids Bile Biomimetic Materials Digestion Eating Enzyme Assays Fatty Acids Food Food Ingredients Gastric Juice Humans Hydrogen-Ion Concentration Hydrolysis Intestines Mouth Oligosaccharides Peptide Fragments Saliva Stomach Models, Biological Avaliação do Impacte em Saúde Composição dos Alimentos |
description |
Developing a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-07-10T14:16:19Z 2019-04 2019-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/6441 |
url |
http://hdl.handle.net/10400.18/6441 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Nat Protoc. 2019 Apr;14(4):991-1014. doi: 10.1038/s41596-018-0119-1. Epub 2019 Mar 18 1754-2189 10.1038/s41596-018-0119-1 |
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info:eu-repo/semantics/embargoedAccess |
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embargoedAccess |
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application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Nature Research |
publisher.none.fl_str_mv |
Nature Research |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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