Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://doi.org/10.1002/mbo3.623 |
Resumo: | Fundacao para a Ciencia e a Tecnologia, Grant/Award Number: ERDF under the PT2020 Partnership Agreement (POCI-, IF/00265/2015, PTDC/CVT-EPI/6685/2014, SFRH/BD/118350/2016, UID/MAR/04292/2013 and UID/Multi/04378/2013; Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO; FCT/MEC, Grant/Award Number: UID/Multi/04378/2013 |
id |
RCAP_29d57259dd699fa4bc8b02427cfcdb40 |
---|---|
oai_identifier_str |
oai:run.unl.pt:10362/82922 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological modelsbovinehost adhesion/internalizationStreptococcus dysgalactiae subsp. dysgalactiaesystemic infectionzebrafishMicrobiologySDG 3 - Good Health and Well-beingFundacao para a Ciencia e a Tecnologia, Grant/Award Number: ERDF under the PT2020 Partnership Agreement (POCI-, IF/00265/2015, PTDC/CVT-EPI/6685/2014, SFRH/BD/118350/2016, UID/MAR/04292/2013 and UID/Multi/04378/2013; Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO; FCT/MEC, Grant/Award Number: UID/Multi/04378/2013Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) is a major cause of bovine mastitis and has been regarded as an animal-restricted pathogen, although rare infections have been described in humans. Previous studies revealed the presence of virulence genes encoded by phages of the human pathogen Group A Streptococcus pyogenes (GAS) in SDSD isolated from the milk of bovine udder with mastitis. The isolates SDSD VSD5 and VSD13 could adhere and internalize human primary keratinocyte cells, suggesting a possible human infection potential of bovine isolates. In this work, the in vitro and in vivo potential of SDSD to internalize/adhere human cells of the respiratory track and zebrafish as biological models was evaluated. Our results showed that, in vitro, bovine SDSD strains could interact and internalize human respiratory cell lines and that this internalization was dependent on an active transport mechanism and that, in vivo, SDSD are able to cause invasive infections producing zebrafish morbidity and mortality. The infectious potential of these isolates showed to be isolate-specific and appeared to be independent of the presence or absence of GAS phage-encoded virulence genes. Although the infection ability of the bovine SDSD strains was not as strong as the human pathogenic S. pyogenes in the zebrafish model, results suggested that these SDSD isolates are able to interact with human cells and infect zebrafish, a vertebrate infectious model, emerging as pathogens with zoonotic capability.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaDQ - Departamento de QuímicaDCEA - Departamento de Ciências e Engenharia do AmbienteMARE - Centro de Ciências do Mar e do AmbienteRUNAlves-Barroco, CinthiaRoma-Rodrigues, CatarinaRaposo, Luís R.Brás, CatarinaDiniz, MárioCaço, JoãoCosta, Pedro M.Santos-Sanches, IldaFernandes, Alexandra R.2019-10-01T22:30:49Z2019-01-012019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1002/mbo3.623engPURE: 11377432http://www.scopus.com/inward/record.url?scp=85060250873&partnerID=8YFLogxKhttps://doi.org/10.1002/mbo3.623info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:36:49Zoai:run.unl.pt:10362/82922Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:36:14.100268Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models |
title |
Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models |
spellingShingle |
Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models Alves-Barroco, Cinthia bovine host adhesion/internalization Streptococcus dysgalactiae subsp. dysgalactiae systemic infection zebrafish Microbiology SDG 3 - Good Health and Well-being |
title_short |
Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models |
title_full |
Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models |
title_fullStr |
Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models |
title_full_unstemmed |
Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models |
title_sort |
Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models |
author |
Alves-Barroco, Cinthia |
author_facet |
Alves-Barroco, Cinthia Roma-Rodrigues, Catarina Raposo, Luís R. Brás, Catarina Diniz, Mário Caço, João Costa, Pedro M. Santos-Sanches, Ilda Fernandes, Alexandra R. |
author_role |
author |
author2 |
Roma-Rodrigues, Catarina Raposo, Luís R. Brás, Catarina Diniz, Mário Caço, João Costa, Pedro M. Santos-Sanches, Ilda Fernandes, Alexandra R. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DCV - Departamento de Ciências da Vida DQ - Departamento de Química DCEA - Departamento de Ciências e Engenharia do Ambiente MARE - Centro de Ciências do Mar e do Ambiente RUN |
dc.contributor.author.fl_str_mv |
Alves-Barroco, Cinthia Roma-Rodrigues, Catarina Raposo, Luís R. Brás, Catarina Diniz, Mário Caço, João Costa, Pedro M. Santos-Sanches, Ilda Fernandes, Alexandra R. |
dc.subject.por.fl_str_mv |
bovine host adhesion/internalization Streptococcus dysgalactiae subsp. dysgalactiae systemic infection zebrafish Microbiology SDG 3 - Good Health and Well-being |
topic |
bovine host adhesion/internalization Streptococcus dysgalactiae subsp. dysgalactiae systemic infection zebrafish Microbiology SDG 3 - Good Health and Well-being |
description |
Fundacao para a Ciencia e a Tecnologia, Grant/Award Number: ERDF under the PT2020 Partnership Agreement (POCI-, IF/00265/2015, PTDC/CVT-EPI/6685/2014, SFRH/BD/118350/2016, UID/MAR/04292/2013 and UID/Multi/04378/2013; Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO; FCT/MEC, Grant/Award Number: UID/Multi/04378/2013 |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-01T22:30:49Z 2019-01-01 2019-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://doi.org/10.1002/mbo3.623 |
url |
https://doi.org/10.1002/mbo3.623 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PURE: 11377432 http://www.scopus.com/inward/record.url?scp=85060250873&partnerID=8YFLogxK https://doi.org/10.1002/mbo3.623 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799137981757390848 |