Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models

Detalhes bibliográficos
Autor(a) principal: Alves-Barroco, Cinthia
Data de Publicação: 2019
Outros Autores: Roma-Rodrigues, Catarina, Raposo, Luís R., Brás, Catarina, Diniz, Mário, Caço, João, Costa, Pedro M., Santos-Sanches, Ilda, Fernandes, Alexandra R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1002/mbo3.623
Resumo: Fundacao para a Ciencia e a Tecnologia, Grant/Award Number: ERDF under the PT2020 Partnership Agreement (POCI-, IF/00265/2015, PTDC/CVT-EPI/6685/2014, SFRH/BD/118350/2016, UID/MAR/04292/2013 and UID/Multi/04378/2013; Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO; FCT/MEC, Grant/Award Number: UID/Multi/04378/2013
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spelling Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological modelsbovinehost adhesion/internalizationStreptococcus dysgalactiae subsp. dysgalactiaesystemic infectionzebrafishMicrobiologySDG 3 - Good Health and Well-beingFundacao para a Ciencia e a Tecnologia, Grant/Award Number: ERDF under the PT2020 Partnership Agreement (POCI-, IF/00265/2015, PTDC/CVT-EPI/6685/2014, SFRH/BD/118350/2016, UID/MAR/04292/2013 and UID/Multi/04378/2013; Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO; FCT/MEC, Grant/Award Number: UID/Multi/04378/2013Streptococcus dysgalactiae subsp. dysgalactiae (SDSD) is a major cause of bovine mastitis and has been regarded as an animal-restricted pathogen, although rare infections have been described in humans. Previous studies revealed the presence of virulence genes encoded by phages of the human pathogen Group A Streptococcus pyogenes (GAS) in SDSD isolated from the milk of bovine udder with mastitis. The isolates SDSD VSD5 and VSD13 could adhere and internalize human primary keratinocyte cells, suggesting a possible human infection potential of bovine isolates. In this work, the in vitro and in vivo potential of SDSD to internalize/adhere human cells of the respiratory track and zebrafish as biological models was evaluated. Our results showed that, in vitro, bovine SDSD strains could interact and internalize human respiratory cell lines and that this internalization was dependent on an active transport mechanism and that, in vivo, SDSD are able to cause invasive infections producing zebrafish morbidity and mortality. The infectious potential of these isolates showed to be isolate-specific and appeared to be independent of the presence or absence of GAS phage-encoded virulence genes. Although the infection ability of the bovine SDSD strains was not as strong as the human pathogenic S. pyogenes in the zebrafish model, results suggested that these SDSD isolates are able to interact with human cells and infect zebrafish, a vertebrate infectious model, emerging as pathogens with zoonotic capability.UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaDQ - Departamento de QuímicaDCEA - Departamento de Ciências e Engenharia do AmbienteMARE - Centro de Ciências do Mar e do AmbienteRUNAlves-Barroco, CinthiaRoma-Rodrigues, CatarinaRaposo, Luís R.Brás, CatarinaDiniz, MárioCaço, JoãoCosta, Pedro M.Santos-Sanches, IldaFernandes, Alexandra R.2019-10-01T22:30:49Z2019-01-012019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1002/mbo3.623engPURE: 11377432http://www.scopus.com/inward/record.url?scp=85060250873&partnerID=8YFLogxKhttps://doi.org/10.1002/mbo3.623info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:36:49Zoai:run.unl.pt:10362/82922Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:36:14.100268Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models
title Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models
spellingShingle Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models
Alves-Barroco, Cinthia
bovine
host adhesion/internalization
Streptococcus dysgalactiae subsp. dysgalactiae
systemic infection
zebrafish
Microbiology
SDG 3 - Good Health and Well-being
title_short Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models
title_full Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models
title_fullStr Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models
title_full_unstemmed Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models
title_sort Streptococcus dysgalactiae subsp. dysgalactiae isolated from milk of the bovine udder as emerging pathogens: In vitro and in vivo infection of human cells and zebrafish as biological models
author Alves-Barroco, Cinthia
author_facet Alves-Barroco, Cinthia
Roma-Rodrigues, Catarina
Raposo, Luís R.
Brás, Catarina
Diniz, Mário
Caço, João
Costa, Pedro M.
Santos-Sanches, Ilda
Fernandes, Alexandra R.
author_role author
author2 Roma-Rodrigues, Catarina
Raposo, Luís R.
Brás, Catarina
Diniz, Mário
Caço, João
Costa, Pedro M.
Santos-Sanches, Ilda
Fernandes, Alexandra R.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
DQ - Departamento de Química
DCEA - Departamento de Ciências e Engenharia do Ambiente
MARE - Centro de Ciências do Mar e do Ambiente
RUN
dc.contributor.author.fl_str_mv Alves-Barroco, Cinthia
Roma-Rodrigues, Catarina
Raposo, Luís R.
Brás, Catarina
Diniz, Mário
Caço, João
Costa, Pedro M.
Santos-Sanches, Ilda
Fernandes, Alexandra R.
dc.subject.por.fl_str_mv bovine
host adhesion/internalization
Streptococcus dysgalactiae subsp. dysgalactiae
systemic infection
zebrafish
Microbiology
SDG 3 - Good Health and Well-being
topic bovine
host adhesion/internalization
Streptococcus dysgalactiae subsp. dysgalactiae
systemic infection
zebrafish
Microbiology
SDG 3 - Good Health and Well-being
description Fundacao para a Ciencia e a Tecnologia, Grant/Award Number: ERDF under the PT2020 Partnership Agreement (POCI-, IF/00265/2015, PTDC/CVT-EPI/6685/2014, SFRH/BD/118350/2016, UID/MAR/04292/2013 and UID/Multi/04378/2013; Unidade de Ciencias Biomoleculares Aplicadas-UCIBIO; FCT/MEC, Grant/Award Number: UID/Multi/04378/2013
publishDate 2019
dc.date.none.fl_str_mv 2019-10-01T22:30:49Z
2019-01-01
2019-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1002/mbo3.623
url https://doi.org/10.1002/mbo3.623
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 11377432
http://www.scopus.com/inward/record.url?scp=85060250873&partnerID=8YFLogxK
https://doi.org/10.1002/mbo3.623
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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