Antithrombotic and hemocompatible properties of nanostructured coatings assembled from block copolymers
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/74468 |
Resumo: | We describe the antithrombotic properties of nanopatterned coatings created by self-assembly of poly(styrene-block-2-vinylpyridine) (PS-b-P2VP) with different molecular weights. By changing the assembly conditions, we obtained nanopatterns that differ by their morphology (size and shape of the nanopattern) and chemistry. The surface exposition of P2VP block allowed quaternization, i.e. introduction of positive surface charge and following electrostatic deposition of heparin. Proteins (albumin and fibrinogen) adsorption, platelet adhesion and activation, cytocompatibility, and reendothelization capacity of the coatings were assessed and discussed in a function of the nanopattern morphology and chemistry. We found that quaternization results in excellent antithrombotic and hemocompatible properties comparable to heparinization by hampering the fibrinogen adhesion and platelet activation. In the case of quaternization, this effect depends on the size of the polymer blocks, while all heparinized patterns had similar performance showing that heparin surface coverage of 40 % is enough to improve substantially the hemocompatibility. |
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Antithrombotic and hemocompatible properties of nanostructured coatings assembled from block copolymersAntithromboticBlock CopolymerHemocompatibleNanopatternQuaternizationScience & TechnologyWe describe the antithrombotic properties of nanopatterned coatings created by self-assembly of poly(styrene-block-2-vinylpyridine) (PS-b-P2VP) with different molecular weights. By changing the assembly conditions, we obtained nanopatterns that differ by their morphology (size and shape of the nanopattern) and chemistry. The surface exposition of P2VP block allowed quaternization, i.e. introduction of positive surface charge and following electrostatic deposition of heparin. Proteins (albumin and fibrinogen) adsorption, platelet adhesion and activation, cytocompatibility, and reendothelization capacity of the coatings were assessed and discussed in a function of the nanopattern morphology and chemistry. We found that quaternization results in excellent antithrombotic and hemocompatible properties comparable to heparinization by hampering the fibrinogen adhesion and platelet activation. In the case of quaternization, this effect depends on the size of the polymer blocks, while all heparinized patterns had similar performance showing that heparin surface coverage of 40 % is enough to improve substantially the hemocompatibility.Portuguese Foundation for Science and Technology for the grants PTDC/QUI-POL/28117/2017, PD/BD/128085/2016, and CEECIND/00814 /2017.ElsevierUniversidade do MinhoFontelo, R.Soares da Costa, D.Reis, R. L.Novoa-Carballal, RamonPashkuleva, I.20222022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/74468engFontelo, R., Soares da Costa, D., Reis, R. L., Novoa-Carballal, R., & Pashkuleva, I. (2022, February). Antithrombotic and hemocompatible properties of nanostructured coatings assembled from block copolymers. Journal of Colloid and Interface Science. Elsevier BV. http://doi.org/10.1016/j.jcis.2021.10.0760021-979710.1016/j.jcis.2021.10.07634742077https://doi.org/10.1016/j.jcis.2021.10.076info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:02:34Zoai:repositorium.sdum.uminho.pt:1822/74468Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:52:33.467806Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Antithrombotic and hemocompatible properties of nanostructured coatings assembled from block copolymers |
title |
Antithrombotic and hemocompatible properties of nanostructured coatings assembled from block copolymers |
spellingShingle |
Antithrombotic and hemocompatible properties of nanostructured coatings assembled from block copolymers Fontelo, R. Antithrombotic Block Copolymer Hemocompatible Nanopattern Quaternization Science & Technology |
title_short |
Antithrombotic and hemocompatible properties of nanostructured coatings assembled from block copolymers |
title_full |
Antithrombotic and hemocompatible properties of nanostructured coatings assembled from block copolymers |
title_fullStr |
Antithrombotic and hemocompatible properties of nanostructured coatings assembled from block copolymers |
title_full_unstemmed |
Antithrombotic and hemocompatible properties of nanostructured coatings assembled from block copolymers |
title_sort |
Antithrombotic and hemocompatible properties of nanostructured coatings assembled from block copolymers |
author |
Fontelo, R. |
author_facet |
Fontelo, R. Soares da Costa, D. Reis, R. L. Novoa-Carballal, Ramon Pashkuleva, I. |
author_role |
author |
author2 |
Soares da Costa, D. Reis, R. L. Novoa-Carballal, Ramon Pashkuleva, I. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Fontelo, R. Soares da Costa, D. Reis, R. L. Novoa-Carballal, Ramon Pashkuleva, I. |
dc.subject.por.fl_str_mv |
Antithrombotic Block Copolymer Hemocompatible Nanopattern Quaternization Science & Technology |
topic |
Antithrombotic Block Copolymer Hemocompatible Nanopattern Quaternization Science & Technology |
description |
We describe the antithrombotic properties of nanopatterned coatings created by self-assembly of poly(styrene-block-2-vinylpyridine) (PS-b-P2VP) with different molecular weights. By changing the assembly conditions, we obtained nanopatterns that differ by their morphology (size and shape of the nanopattern) and chemistry. The surface exposition of P2VP block allowed quaternization, i.e. introduction of positive surface charge and following electrostatic deposition of heparin. Proteins (albumin and fibrinogen) adsorption, platelet adhesion and activation, cytocompatibility, and reendothelization capacity of the coatings were assessed and discussed in a function of the nanopattern morphology and chemistry. We found that quaternization results in excellent antithrombotic and hemocompatible properties comparable to heparinization by hampering the fibrinogen adhesion and platelet activation. In the case of quaternization, this effect depends on the size of the polymer blocks, while all heparinized patterns had similar performance showing that heparin surface coverage of 40 % is enough to improve substantially the hemocompatibility. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022 2022-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/74468 |
url |
https://hdl.handle.net/1822/74468 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Fontelo, R., Soares da Costa, D., Reis, R. L., Novoa-Carballal, R., & Pashkuleva, I. (2022, February). Antithrombotic and hemocompatible properties of nanostructured coatings assembled from block copolymers. Journal of Colloid and Interface Science. Elsevier BV. http://doi.org/10.1016/j.jcis.2021.10.076 0021-9797 10.1016/j.jcis.2021.10.076 34742077 https://doi.org/10.1016/j.jcis.2021.10.076 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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