Cytotoxic potential of graphene oxide in human lung Adenocarcinoma cell line

• Autor(a) principal: Viegas, Juliane Oliveira
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10773/22356
• topic: Oxido de grafeno - Citotoxicidade; Cancro do pulmão; Citometria de fluxo; Patologia celular

Graphene oxide (GO) is a compound with application in several fields, especially biomedicine and environment, due to its unique properties which confer excellent characteristics. Despite the nowadays wide application of nanomaterials, the lack of information regarding the risks to human health and the environment is still remaining. Consequently, the investigation about the toxicity of nanoparticles must be a priority. The lung is one of the main routes of entry for nanoparticles into the body, therefore, the aim of this study is to evaluate the cytotoxic potential of graphene oxide in lung, using as model the human carcinoma epithelial cell line (A549). The morphology and viability of A549 cells were evaluated after 24h of exposure to GO at concentrations from 10μg/ml to 200μg/ml. The uptake of GO and the production of reactive oxygen species were also investigated by flow cytometry. The results suggest that GO has no obvious toxicity to A549 cells when assessed by WST-8 assay, though the cell cycle showed a slightly alteration in the S and G2 phase at 50μg/mL with arrest at G2 phase. Also, GO showed an increasing in the ROS production at the lowest doses (10ug/mL and 37μg/mL). The intracellular uptake increased for the highest concentration. Together these results suggest that this form of GO shows biocompatibility for lung cells.