Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells

Detalhes bibliográficos
Autor(a) principal: Jesus, Ana Rita
Data de Publicação: 2022
Outros Autores: Duarte, Ana Rita C., Paiva, Alexandre
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/162116
Resumo: This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme, under grant agreement No ERC-2016-CoG 725034. Publisher Copyright: © 2022, The Author(s).
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spelling Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cellsGeneralThis project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme, under grant agreement No ERC-2016-CoG 725034. Publisher Copyright: © 2022, The Author(s).In this work we present the potential of Natural Deep Eutectic Systems (NADES) as new vitrification media for the cryopreservation of mammalian cells. Several NADES composed of natural metabolites were prepared and tested as CPAs in two cell lines, L929 and HacaT cells. After the harvesting, cells were mixed with the eutectic systems, and frozen directly into liquid nitrogen to achieve a vitreous state. Then, the cells were thawed and it was observed that NADES were able to exert a significant cryoprotective effect in L929 cells, when compared with DMSO or in the absence of a CPA. For HacaT cells, only a eutectic system showed a slightly improvement in cell survival, while DMSO caused complete cell death. Moreover, the thermal behaviour of the best systems was studied for further understanding the protective properties of NADES as CPAs, and have shown a significant difference in terms of Tm and Tc when compared with DMSO and water. Additionally, the results obtained showed that NADES can be maintained in the growth media after the thawing step, without compromising cell viability. In summary, we have shown the great potential of NADES to be used as CPAs for the cryopreservation of different cell types, using the vitrification method.LAQV@REQUIMTERUNJesus, Ana RitaDuarte, Ana Rita C.Paiva, Alexandre2024-01-10T23:25:23Z2022-122022-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://hdl.handle.net/10362/162116eng2045-2322PURE: 80867065https://doi.org/10.1038/s41598-022-12365-4info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:44:55Zoai:run.unl.pt:10362/162116Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:58:45.615045Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells
title Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells
spellingShingle Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells
Jesus, Ana Rita
General
title_short Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells
title_full Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells
title_fullStr Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells
title_full_unstemmed Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells
title_sort Use of natural deep eutectic systems as new cryoprotectant agents in the vitrification of mammalian cells
author Jesus, Ana Rita
author_facet Jesus, Ana Rita
Duarte, Ana Rita C.
Paiva, Alexandre
author_role author
author2 Duarte, Ana Rita C.
Paiva, Alexandre
author2_role author
author
dc.contributor.none.fl_str_mv LAQV@REQUIMTE
RUN
dc.contributor.author.fl_str_mv Jesus, Ana Rita
Duarte, Ana Rita C.
Paiva, Alexandre
dc.subject.por.fl_str_mv General
topic General
description This project has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 Research and Innovation Programme, under grant agreement No ERC-2016-CoG 725034. Publisher Copyright: © 2022, The Author(s).
publishDate 2022
dc.date.none.fl_str_mv 2022-12
2022-12-01T00:00:00Z
2024-01-10T23:25:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/162116
url http://hdl.handle.net/10362/162116
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2045-2322
PURE: 80867065
https://doi.org/10.1038/s41598-022-12365-4
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 9
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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