Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans

Detalhes bibliográficos
Autor(a) principal: Marques, Cátia Filipa Saraiva
Data de Publicação: 2019
Outros Autores: Belas, Adriana, Aboim, Catarina, Trigueiro, Graça, Cavaco-Silva, Patrícia, Gama, Luis, Pomba, C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.5/22146
Resumo: Research Areas: Microbiology. Veterinary Sciences
id RCAP_2c09a6834fb93fd397f790e26ff1d8ce
oai_identifier_str oai:www.repository.utl.pt:10400.5/22146
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humansProteus mirabilisUrinary tract infectionCompanion animalHumanClonal relatednessResearch Areas: Microbiology. Veterinary SciencesABSTRACT - Proteus mirabilis is a major cause of urinary tract infection (UTI) in humans and companion animals. This study aimed to evaluate the antimicrobial resistance, virulence and clonal relatedness of P. mirabilis isolated from dogs, cats and humans with UTI. P. mirabilis isolated from companion animals (N = 107) and humans (N = 76) with UTI were compared by PFGE analysis after overnight Nod macro-restriction using Dice/UPGMA with a 1.5% tolerance. Strains were characterized for antimicrobial resistance by disk diffusion. Twenty-four resistance genes and four virulence genes were screened by PCR. Thirty-nine clusters (similarity > 80%) and 73 single pulse-types were detected. Nine clusters included P. mirabilis isolated from community and hospital patients, including strains with 100% similarity. A high number of clusters (43.6%, n = 17/39) included strains from companion animals and humans. Similarity between some companion animal and human strains varied between 80-100%. One strain from a dog was 100% similar to one human community-acquired P. mirabilis. One P. mirabilis from a cat was found to be 94.7% and 92.4% similar to community and hospital patient strains, respectively. P. mirabilis CMY-2-producers did not cluster all together. Nevertheless, cluster C36 included five P. mirabilis from companion animals (similarity 85.8%-95.7%), of which, four (80%) were multidrug-resistant CMY-2-producers. This study shows that companion animals and humans become infected with closely related P. mirabilis strains. The high number of clusters containing companion animals and human strains points to the zoonotic nature of P. mirabilis. These results underline the potential role of companion animals as reservoirs and in the dissemination of uropathogenic P. mirabilis to humans and vice versa.ELSEVIERRepositório da Universidade de LisboaMarques, Cátia Filipa SaraivaBelas, AdrianaAboim, CatarinaTrigueiro, GraçaCavaco-Silva, PatríciaGama, LuisPomba, C.2021-10-06T15:08:42Z2019-012019-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/22146engMarques C, Belas A, Aboim C, Trigueiro G, Cavaco-Silva P, Gama LT, Pomba C. 2019. Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans. Veterinary Microbiology. 228(1):77-820378-113510.1016/j.vetmic.2018.10.015info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:51:42Zoai:www.repository.utl.pt:10400.5/22146Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:06:38.164956Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
title Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
spellingShingle Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
Marques, Cátia Filipa Saraiva
Proteus mirabilis
Urinary tract infection
Companion animal
Human
Clonal relatedness
title_short Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
title_full Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
title_fullStr Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
title_full_unstemmed Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
title_sort Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans
author Marques, Cátia Filipa Saraiva
author_facet Marques, Cátia Filipa Saraiva
Belas, Adriana
Aboim, Catarina
Trigueiro, Graça
Cavaco-Silva, Patrícia
Gama, Luis
Pomba, C.
author_role author
author2 Belas, Adriana
Aboim, Catarina
Trigueiro, Graça
Cavaco-Silva, Patrícia
Gama, Luis
Pomba, C.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Marques, Cátia Filipa Saraiva
Belas, Adriana
Aboim, Catarina
Trigueiro, Graça
Cavaco-Silva, Patrícia
Gama, Luis
Pomba, C.
dc.subject.por.fl_str_mv Proteus mirabilis
Urinary tract infection
Companion animal
Human
Clonal relatedness
topic Proteus mirabilis
Urinary tract infection
Companion animal
Human
Clonal relatedness
description Research Areas: Microbiology. Veterinary Sciences
publishDate 2019
dc.date.none.fl_str_mv 2019-01
2019-01-01T00:00:00Z
2021-10-06T15:08:42Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.5/22146
url http://hdl.handle.net/10400.5/22146
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Marques C, Belas A, Aboim C, Trigueiro G, Cavaco-Silva P, Gama LT, Pomba C. 2019. Clonal relatedness of Proteus mirabilis strains causing urinary tract infections in companion animals and humans. Veterinary Microbiology. 228(1):77-82
0378-1135
10.1016/j.vetmic.2018.10.015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ELSEVIER
publisher.none.fl_str_mv ELSEVIER
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1817550556398354432

Registros relacionados