Human and companion animal Proteus mirabilis sharing

Detalhes bibliográficos
Autor(a) principal: Marques, Cátia
Data de Publicação: 2021
Outros Autores: Belas, Adriana, Menezes, Juliana, Silva, Joana Moreira da, Silva, Patrícia Cavaco, Trigueiro, Graça, Gama, Luís Telo da, Pomba, Constança
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10437/12355
Resumo: Proteus mirabilis is an important pathogen that is associated with urinary tract infections. This study aims to determine the colonization and sharing of P. mirabilis between healthy companion animals and humans that are living together and to evaluate the clonal relatedness of the fecal and clinical stains. Eighteen households (24 humans, 18 dogs, 8 cats) with at least one human–animal pair were studied. Fecal samples were plated onto MacConkey and Hektoen agar and P. mirabilis PFGE analysis (NotI; Dice/UPGMA; 1.5% tolerance) was conducted for the households with multiple positive participants. Antimicrobial-resistance was tested according to CLSI. The fecal P. mirabilis pulse-types were compared with uropathogenic clinical strains (n = 183). Forty-nine P. mirabilis were isolated from eight households. The percentage of colonization in the dogs (44.4%, n = 8/18) was significantly higher (p = 0.0329) than in the humans (12.5%, n = 3/24). Three households had multiple colonized participants. One human–dog pair shared related P. mirabilis strains, which clustered with a clinical strain of animal origin (82.5%). One fecal P. mirabilis strain, from a dog, clustered with two human community-acquired clinical strains (80.9%, 88.9%). To our knowledge, this is the first report of dogs and humans living in close contact and sharing related P. mirabilis strains. The high frequency of colonization in the dogs underlines their possible role as P. mirabilis reservoirs for humans and other dogs.
id RCAP_9d2a9f7e7aa993bb68faf02954f6a9f6
oai_identifier_str oai:recil.ensinolusofona.pt:10437/12355
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Human and companion animal Proteus mirabilis sharingBIOLOGIAMICROBIOLOGIAANIMAIS DE COMPANHIAHOMEMPROTEUS MIRABILISBIOLOGYMICROBIOLOGYCOMPANION ANIMALSMANPROTEUS MIRABILISProteus mirabilis is an important pathogen that is associated with urinary tract infections. This study aims to determine the colonization and sharing of P. mirabilis between healthy companion animals and humans that are living together and to evaluate the clonal relatedness of the fecal and clinical stains. Eighteen households (24 humans, 18 dogs, 8 cats) with at least one human–animal pair were studied. Fecal samples were plated onto MacConkey and Hektoen agar and P. mirabilis PFGE analysis (NotI; Dice/UPGMA; 1.5% tolerance) was conducted for the households with multiple positive participants. Antimicrobial-resistance was tested according to CLSI. The fecal P. mirabilis pulse-types were compared with uropathogenic clinical strains (n = 183). Forty-nine P. mirabilis were isolated from eight households. The percentage of colonization in the dogs (44.4%, n = 8/18) was significantly higher (p = 0.0329) than in the humans (12.5%, n = 3/24). Three households had multiple colonized participants. One human–dog pair shared related P. mirabilis strains, which clustered with a clinical strain of animal origin (82.5%). One fecal P. mirabilis strain, from a dog, clustered with two human community-acquired clinical strains (80.9%, 88.9%). To our knowledge, this is the first report of dogs and humans living in close contact and sharing related P. mirabilis strains. The high frequency of colonization in the dogs underlines their possible role as P. mirabilis reservoirs for humans and other dogs.2022-01-10T12:23:52Z2021-01-01T00:00:00Z2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10437/12355enghttps://doi.org/10.3390/microbiolres13010003Marques, CátiaBelas, AdrianaMenezes, JulianaSilva, Joana Moreira daSilva, Patrícia CavacoTrigueiro, GraçaGama, Luís Telo daPomba, Constançainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-09T14:11:08Zoai:recil.ensinolusofona.pt:10437/12355Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:17:44.543705Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Human and companion animal Proteus mirabilis sharing
title Human and companion animal Proteus mirabilis sharing
spellingShingle Human and companion animal Proteus mirabilis sharing
Marques, Cátia
BIOLOGIA
MICROBIOLOGIA
ANIMAIS DE COMPANHIA
HOMEM
PROTEUS MIRABILIS
BIOLOGY
MICROBIOLOGY
COMPANION ANIMALS
MAN
PROTEUS MIRABILIS
title_short Human and companion animal Proteus mirabilis sharing
title_full Human and companion animal Proteus mirabilis sharing
title_fullStr Human and companion animal Proteus mirabilis sharing
title_full_unstemmed Human and companion animal Proteus mirabilis sharing
title_sort Human and companion animal Proteus mirabilis sharing
author Marques, Cátia
author_facet Marques, Cátia
Belas, Adriana
Menezes, Juliana
Silva, Joana Moreira da
Silva, Patrícia Cavaco
Trigueiro, Graça
Gama, Luís Telo da
Pomba, Constança
author_role author
author2 Belas, Adriana
Menezes, Juliana
Silva, Joana Moreira da
Silva, Patrícia Cavaco
Trigueiro, Graça
Gama, Luís Telo da
Pomba, Constança
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marques, Cátia
Belas, Adriana
Menezes, Juliana
Silva, Joana Moreira da
Silva, Patrícia Cavaco
Trigueiro, Graça
Gama, Luís Telo da
Pomba, Constança
dc.subject.por.fl_str_mv BIOLOGIA
MICROBIOLOGIA
ANIMAIS DE COMPANHIA
HOMEM
PROTEUS MIRABILIS
BIOLOGY
MICROBIOLOGY
COMPANION ANIMALS
MAN
PROTEUS MIRABILIS
topic BIOLOGIA
MICROBIOLOGIA
ANIMAIS DE COMPANHIA
HOMEM
PROTEUS MIRABILIS
BIOLOGY
MICROBIOLOGY
COMPANION ANIMALS
MAN
PROTEUS MIRABILIS
description Proteus mirabilis is an important pathogen that is associated with urinary tract infections. This study aims to determine the colonization and sharing of P. mirabilis between healthy companion animals and humans that are living together and to evaluate the clonal relatedness of the fecal and clinical stains. Eighteen households (24 humans, 18 dogs, 8 cats) with at least one human–animal pair were studied. Fecal samples were plated onto MacConkey and Hektoen agar and P. mirabilis PFGE analysis (NotI; Dice/UPGMA; 1.5% tolerance) was conducted for the households with multiple positive participants. Antimicrobial-resistance was tested according to CLSI. The fecal P. mirabilis pulse-types were compared with uropathogenic clinical strains (n = 183). Forty-nine P. mirabilis were isolated from eight households. The percentage of colonization in the dogs (44.4%, n = 8/18) was significantly higher (p = 0.0329) than in the humans (12.5%, n = 3/24). Three households had multiple colonized participants. One human–dog pair shared related P. mirabilis strains, which clustered with a clinical strain of animal origin (82.5%). One fecal P. mirabilis strain, from a dog, clustered with two human community-acquired clinical strains (80.9%, 88.9%). To our knowledge, this is the first report of dogs and humans living in close contact and sharing related P. mirabilis strains. The high frequency of colonization in the dogs underlines their possible role as P. mirabilis reservoirs for humans and other dogs.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01T00:00:00Z
2021
2022-01-10T12:23:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10437/12355
url http://hdl.handle.net/10437/12355
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.3390/microbiolres13010003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799131274573512704