Human and companion animal Proteus mirabilis sharing
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10437/12355 |
Resumo: | Proteus mirabilis is an important pathogen that is associated with urinary tract infections. This study aims to determine the colonization and sharing of P. mirabilis between healthy companion animals and humans that are living together and to evaluate the clonal relatedness of the fecal and clinical stains. Eighteen households (24 humans, 18 dogs, 8 cats) with at least one human–animal pair were studied. Fecal samples were plated onto MacConkey and Hektoen agar and P. mirabilis PFGE analysis (NotI; Dice/UPGMA; 1.5% tolerance) was conducted for the households with multiple positive participants. Antimicrobial-resistance was tested according to CLSI. The fecal P. mirabilis pulse-types were compared with uropathogenic clinical strains (n = 183). Forty-nine P. mirabilis were isolated from eight households. The percentage of colonization in the dogs (44.4%, n = 8/18) was significantly higher (p = 0.0329) than in the humans (12.5%, n = 3/24). Three households had multiple colonized participants. One human–dog pair shared related P. mirabilis strains, which clustered with a clinical strain of animal origin (82.5%). One fecal P. mirabilis strain, from a dog, clustered with two human community-acquired clinical strains (80.9%, 88.9%). To our knowledge, this is the first report of dogs and humans living in close contact and sharing related P. mirabilis strains. The high frequency of colonization in the dogs underlines their possible role as P. mirabilis reservoirs for humans and other dogs. |
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Human and companion animal Proteus mirabilis sharingBIOLOGIAMICROBIOLOGIAANIMAIS DE COMPANHIAHOMEMPROTEUS MIRABILISBIOLOGYMICROBIOLOGYCOMPANION ANIMALSMANPROTEUS MIRABILISProteus mirabilis is an important pathogen that is associated with urinary tract infections. This study aims to determine the colonization and sharing of P. mirabilis between healthy companion animals and humans that are living together and to evaluate the clonal relatedness of the fecal and clinical stains. Eighteen households (24 humans, 18 dogs, 8 cats) with at least one human–animal pair were studied. Fecal samples were plated onto MacConkey and Hektoen agar and P. mirabilis PFGE analysis (NotI; Dice/UPGMA; 1.5% tolerance) was conducted for the households with multiple positive participants. Antimicrobial-resistance was tested according to CLSI. The fecal P. mirabilis pulse-types were compared with uropathogenic clinical strains (n = 183). Forty-nine P. mirabilis were isolated from eight households. The percentage of colonization in the dogs (44.4%, n = 8/18) was significantly higher (p = 0.0329) than in the humans (12.5%, n = 3/24). Three households had multiple colonized participants. One human–dog pair shared related P. mirabilis strains, which clustered with a clinical strain of animal origin (82.5%). One fecal P. mirabilis strain, from a dog, clustered with two human community-acquired clinical strains (80.9%, 88.9%). To our knowledge, this is the first report of dogs and humans living in close contact and sharing related P. mirabilis strains. The high frequency of colonization in the dogs underlines their possible role as P. mirabilis reservoirs for humans and other dogs.2022-01-10T12:23:52Z2021-01-01T00:00:00Z2021info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10437/12355enghttps://doi.org/10.3390/microbiolres13010003Marques, CátiaBelas, AdrianaMenezes, JulianaSilva, Joana Moreira daSilva, Patrícia CavacoTrigueiro, GraçaGama, Luís Telo daPomba, Constançainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-09T14:11:08Zoai:recil.ensinolusofona.pt:10437/12355Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:17:44.543705Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Human and companion animal Proteus mirabilis sharing |
title |
Human and companion animal Proteus mirabilis sharing |
spellingShingle |
Human and companion animal Proteus mirabilis sharing Marques, Cátia BIOLOGIA MICROBIOLOGIA ANIMAIS DE COMPANHIA HOMEM PROTEUS MIRABILIS BIOLOGY MICROBIOLOGY COMPANION ANIMALS MAN PROTEUS MIRABILIS |
title_short |
Human and companion animal Proteus mirabilis sharing |
title_full |
Human and companion animal Proteus mirabilis sharing |
title_fullStr |
Human and companion animal Proteus mirabilis sharing |
title_full_unstemmed |
Human and companion animal Proteus mirabilis sharing |
title_sort |
Human and companion animal Proteus mirabilis sharing |
author |
Marques, Cátia |
author_facet |
Marques, Cátia Belas, Adriana Menezes, Juliana Silva, Joana Moreira da Silva, Patrícia Cavaco Trigueiro, Graça Gama, Luís Telo da Pomba, Constança |
author_role |
author |
author2 |
Belas, Adriana Menezes, Juliana Silva, Joana Moreira da Silva, Patrícia Cavaco Trigueiro, Graça Gama, Luís Telo da Pomba, Constança |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Marques, Cátia Belas, Adriana Menezes, Juliana Silva, Joana Moreira da Silva, Patrícia Cavaco Trigueiro, Graça Gama, Luís Telo da Pomba, Constança |
dc.subject.por.fl_str_mv |
BIOLOGIA MICROBIOLOGIA ANIMAIS DE COMPANHIA HOMEM PROTEUS MIRABILIS BIOLOGY MICROBIOLOGY COMPANION ANIMALS MAN PROTEUS MIRABILIS |
topic |
BIOLOGIA MICROBIOLOGIA ANIMAIS DE COMPANHIA HOMEM PROTEUS MIRABILIS BIOLOGY MICROBIOLOGY COMPANION ANIMALS MAN PROTEUS MIRABILIS |
description |
Proteus mirabilis is an important pathogen that is associated with urinary tract infections. This study aims to determine the colonization and sharing of P. mirabilis between healthy companion animals and humans that are living together and to evaluate the clonal relatedness of the fecal and clinical stains. Eighteen households (24 humans, 18 dogs, 8 cats) with at least one human–animal pair were studied. Fecal samples were plated onto MacConkey and Hektoen agar and P. mirabilis PFGE analysis (NotI; Dice/UPGMA; 1.5% tolerance) was conducted for the households with multiple positive participants. Antimicrobial-resistance was tested according to CLSI. The fecal P. mirabilis pulse-types were compared with uropathogenic clinical strains (n = 183). Forty-nine P. mirabilis were isolated from eight households. The percentage of colonization in the dogs (44.4%, n = 8/18) was significantly higher (p = 0.0329) than in the humans (12.5%, n = 3/24). Three households had multiple colonized participants. One human–dog pair shared related P. mirabilis strains, which clustered with a clinical strain of animal origin (82.5%). One fecal P. mirabilis strain, from a dog, clustered with two human community-acquired clinical strains (80.9%, 88.9%). To our knowledge, this is the first report of dogs and humans living in close contact and sharing related P. mirabilis strains. The high frequency of colonization in the dogs underlines their possible role as P. mirabilis reservoirs for humans and other dogs. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01T00:00:00Z 2021 2022-01-10T12:23:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10437/12355 |
url |
http://hdl.handle.net/10437/12355 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://doi.org/10.3390/microbiolres13010003 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131274573512704 |