Isothermal Amplification of Nucleic Acids

Detalhes bibliográficos
Autor(a) principal: Oliveira, Beatriz B.
Data de Publicação: 2021
Outros Autores: Veigas, Bruno, Baptista, Pedro Viana
Tipo de documento: Outros
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/174418
Resumo: Publisher Copyright: Copyright © 2021 Oliveira, Veigas and Baptista.
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network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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spelling Isothermal Amplification of Nucleic AcidsThe Race for the Next “Gold Standard”isothermal amplificationNAATnon-pcr amplificationnucleic acids amplificationpoint—of—careAnalytical ChemistryBiomedical EngineeringElectrical and Electronic EngineeringMaterials Science (miscellaneous)Publisher Copyright: Copyright © 2021 Oliveira, Veigas and Baptista.Nucleic acid amplification technologies (NAATs) have become fundamental tools in molecular diagnostics, due to their ability to detect small amounts of target molecules. Since its development, Polymerase Chain Reaction (PCR) has been the most exploited method, being stablished as the “gold standard” technique for DNA amplification. However, the requirement for different working temperatures leads to the need of a thermocycler machine or complex thermal apparatus, which have been preventing its application in novel integrated devices for single workflow and high throughput analysis. Conversely, isothermal amplification methods have been gaining attention, especially for point-of-care diagnosis and applications. These non-PCR based methods have been developed by mimicking the in vivo amplification mechanisms, while performing the amplification with high sensitivity, selectivity and allowing for high-throughput analysis. These favorable capabilities have pushed forward the implementation and commercialization of several platforms that exploit isothermal amplification methods, mostly against virus, bacteria and other pathogens in water, food, environmental and clinical samples. Nevertheless, the future of isothermal amplification methods is still dependent on achieving technical maturity and broader commercialization of enzymes and reagents.DCV - Departamento de Ciências da VidaUCIBIO - Applied Molecular Biosciences UnitRUNOliveira, Beatriz B.Veigas, BrunoBaptista, Pedro Viana2024-10-31T23:07:08Z20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/other22application/pdfhttp://hdl.handle.net/10362/174418eng2673-5067PURE: 99281355https://doi.org/10.3389/fsens.2021.752600info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-11T01:40:51Zoai:run.unl.pt:10362/174418Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-11T01:40:51Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Isothermal Amplification of Nucleic Acids
The Race for the Next “Gold Standard”
title Isothermal Amplification of Nucleic Acids
spellingShingle Isothermal Amplification of Nucleic Acids
Oliveira, Beatriz B.
isothermal amplification
NAAT
non-pcr amplification
nucleic acids amplification
point—of—care
Analytical Chemistry
Biomedical Engineering
Electrical and Electronic Engineering
Materials Science (miscellaneous)
title_short Isothermal Amplification of Nucleic Acids
title_full Isothermal Amplification of Nucleic Acids
title_fullStr Isothermal Amplification of Nucleic Acids
title_full_unstemmed Isothermal Amplification of Nucleic Acids
title_sort Isothermal Amplification of Nucleic Acids
author Oliveira, Beatriz B.
author_facet Oliveira, Beatriz B.
Veigas, Bruno
Baptista, Pedro Viana
author_role author
author2 Veigas, Bruno
Baptista, Pedro Viana
author2_role author
author
dc.contributor.none.fl_str_mv DCV - Departamento de Ciências da Vida
UCIBIO - Applied Molecular Biosciences Unit
RUN
dc.contributor.author.fl_str_mv Oliveira, Beatriz B.
Veigas, Bruno
Baptista, Pedro Viana
dc.subject.por.fl_str_mv isothermal amplification
NAAT
non-pcr amplification
nucleic acids amplification
point—of—care
Analytical Chemistry
Biomedical Engineering
Electrical and Electronic Engineering
Materials Science (miscellaneous)
topic isothermal amplification
NAAT
non-pcr amplification
nucleic acids amplification
point—of—care
Analytical Chemistry
Biomedical Engineering
Electrical and Electronic Engineering
Materials Science (miscellaneous)
description Publisher Copyright: Copyright © 2021 Oliveira, Veigas and Baptista.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
2024-10-31T23:07:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/other
format other
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/174418
url http://hdl.handle.net/10362/174418
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2673-5067
PURE: 99281355
https://doi.org/10.3389/fsens.2021.752600
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 22
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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