Digital microfluidics for nucleic acid amplification

Detalhes bibliográficos
Autor(a) principal: Coelho, Beatriz
Data de Publicação: 2017
Outros Autores: Veigas, Bruno, Fortunato, Elvira, Martins, Rodrigo, Águas, Hugo, Igreja, Rui, Baptista, Pedro V.
Tipo de documento: Outros
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.3390/s17071495
Resumo: This work was funded by FEDER funds through the COMPETE 2020 Program (POCI-01-0145-FEDER-007728). BV thanks SFRH/BPD/124311/2016. PVB thanksWater JPI Proj TRACE (Ref 281715).
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spelling Digital microfluidics for nucleic acid amplificationDigital microfluidicsNucleic acid amplificationPoint-of-care diagnosticsAnalytical ChemistryAtomic and Molecular Physics, and OpticsBiochemistryInstrumentationElectrical and Electronic EngineeringSDG 3 - Good Health and Well-beingThis work was funded by FEDER funds through the COMPETE 2020 Program (POCI-01-0145-FEDER-007728). BV thanks SFRH/BPD/124311/2016. PVB thanksWater JPI Proj TRACE (Ref 281715).Digital Microfluidics (DMF) has emerged as a disruptive methodology for the control and manipulation of low volume droplets. In DMF, each droplet acts as a single reactor, which allows for extensive multiparallelization of biological and chemical reactions at a much smaller scale. DMF devices open entirely new and promising pathways for multiplex analysis and reaction occurring in a miniaturized format, thus allowing for healthcare decentralization from major laboratories to point-of-care with accurate, robust and inexpensive molecular diagnostics. Here, we shall focus on DMF platforms specifically designed for nucleic acid amplification, which is key for molecular diagnostics of several diseases and conditions, from pathogen identification to cancer mutations detection. Particular attention will be given to the device architecture, materials and nucleic acid amplification applications in validated settings.DCM - Departamento de Ciência dos MateriaisCENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)UCIBIO - Applied Molecular Biosciences UnitDCV - Departamento de Ciências da VidaRUNCoelho, BeatrizVeigas, BrunoFortunato, ElviraMartins, RodrigoÁguas, HugoIgreja, RuiBaptista, Pedro V.2019-03-12T23:17:08Z2017-07-012017-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/otherapplication/pdfhttps://doi.org/10.3390/s17071495eng1424-8220PURE: 11472116http://www.scopus.com/inward/record.url?scp=85021393536&partnerID=8YFLogxKhttps://doi.org/10.3390/s17071495info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T17:37:43Zoai:run.unl.pt:10362/63112Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T17:37:43Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Digital microfluidics for nucleic acid amplification
title Digital microfluidics for nucleic acid amplification
spellingShingle Digital microfluidics for nucleic acid amplification
Coelho, Beatriz
Digital microfluidics
Nucleic acid amplification
Point-of-care diagnostics
Analytical Chemistry
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering
SDG 3 - Good Health and Well-being
title_short Digital microfluidics for nucleic acid amplification
title_full Digital microfluidics for nucleic acid amplification
title_fullStr Digital microfluidics for nucleic acid amplification
title_full_unstemmed Digital microfluidics for nucleic acid amplification
title_sort Digital microfluidics for nucleic acid amplification
author Coelho, Beatriz
author_facet Coelho, Beatriz
Veigas, Bruno
Fortunato, Elvira
Martins, Rodrigo
Águas, Hugo
Igreja, Rui
Baptista, Pedro V.
author_role author
author2 Veigas, Bruno
Fortunato, Elvira
Martins, Rodrigo
Águas, Hugo
Igreja, Rui
Baptista, Pedro V.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv DCM - Departamento de Ciência dos Materiais
CENIMAT-i3N - Centro de Investigação de Materiais (Lab. Associado I3N)
UCIBIO - Applied Molecular Biosciences Unit
DCV - Departamento de Ciências da Vida
RUN
dc.contributor.author.fl_str_mv Coelho, Beatriz
Veigas, Bruno
Fortunato, Elvira
Martins, Rodrigo
Águas, Hugo
Igreja, Rui
Baptista, Pedro V.
dc.subject.por.fl_str_mv Digital microfluidics
Nucleic acid amplification
Point-of-care diagnostics
Analytical Chemistry
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering
SDG 3 - Good Health and Well-being
topic Digital microfluidics
Nucleic acid amplification
Point-of-care diagnostics
Analytical Chemistry
Atomic and Molecular Physics, and Optics
Biochemistry
Instrumentation
Electrical and Electronic Engineering
SDG 3 - Good Health and Well-being
description This work was funded by FEDER funds through the COMPETE 2020 Program (POCI-01-0145-FEDER-007728). BV thanks SFRH/BPD/124311/2016. PVB thanksWater JPI Proj TRACE (Ref 281715).
publishDate 2017
dc.date.none.fl_str_mv 2017-07-01
2017-07-01T00:00:00Z
2019-03-12T23:17:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/other
format other
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.3390/s17071495
url https://doi.org/10.3390/s17071495
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1424-8220
PURE: 11472116
http://www.scopus.com/inward/record.url?scp=85021393536&partnerID=8YFLogxK
https://doi.org/10.3390/s17071495
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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