A comprehensive study of herbal blends from portuguese smart-shops: isolation, analysis and toxicological impact

Detalhes bibliográficos
Autor(a) principal: Lopes, Inês Costa
Data de Publicação: 2014
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.26/6714
Resumo: Dissertação de mestrado Erasmus Mundus para obtenção de grau de mestre em Técnicas Laboratoriais Forenses
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spelling A comprehensive study of herbal blends from portuguese smart-shops: isolation, analysis and toxicological impactSynthetic cannabinoidsJWH-018JWH-122JWH-210HPLC-UVLC-MS/MSGC-MSSmoking machineToxicityDissertação de mestrado Erasmus Mundus para obtenção de grau de mestre em Técnicas Laboratoriais ForensesIn the last few years, several samples were found to contain different combinations of multiple synthetic cannabinoids, fact which may end up in a serious health threat since these substances are not fully studied and the toxic as well as the pharmacological effects are not known. The isolation and quantification of psychoactive substances from the herbal blends, known as ‘Spice’, was performed by HPLC-UV and the identification by LC-MS/MS. The HPLC-UV method was validated for the quantification and has proven to be fit for purpose. The sample 1 has shown the coexistence of JWH-122 (29,07mg/g) and JWH-210 (175,9mg/g) while the sample 2 was found to contain one single synthetic cannabinoid, the JWH-018 (149,7mg/g). The sample 3 presented the same multiple psychoactive substances as sample 1, JWH-122 (7,3mg/g) and JWH-210 (129,9mg/g). Since these drugs are mainly smoked, a smoking machine was created* in order to characterize the chemical composition of the pyrolysis. The GC-MS analysis performed to the pyrolysis solutions has shown no degradation of the JWH-018, drug used for the assay. The toxicity impact of the characterized substances was assessed on human neuroblastoma SH-SY5Y cells, applying a combination of multiple synthetic cannabinoids (JWH-122 and JWH-210) and single one (JWH-018). The preliminary results indicate that these psychoactive substances are not toxic to the cells in the concentrations ranging from 0μM to 50μM. *Designed by Dr. Alexandre Quintas and is under a patenting processInstituto Superior de Ciências da Saúde Egas MonizQuintas, AlexandreSoeiro, RitaRepositório ComumLopes, Inês Costa2014-09-18T11:31:20Z2014-07-01T00:00:00Z2014-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10400.26/6714201541505enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-10-06T14:51:29Zoai:comum.rcaap.pt:10400.26/6714Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:06:05.490167Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A comprehensive study of herbal blends from portuguese smart-shops: isolation, analysis and toxicological impact
title A comprehensive study of herbal blends from portuguese smart-shops: isolation, analysis and toxicological impact
spellingShingle A comprehensive study of herbal blends from portuguese smart-shops: isolation, analysis and toxicological impact
Lopes, Inês Costa
Synthetic cannabinoids
JWH-018
JWH-122
JWH-210
HPLC-UV
LC-MS/MS
GC-MS
Smoking machine
Toxicity
title_short A comprehensive study of herbal blends from portuguese smart-shops: isolation, analysis and toxicological impact
title_full A comprehensive study of herbal blends from portuguese smart-shops: isolation, analysis and toxicological impact
title_fullStr A comprehensive study of herbal blends from portuguese smart-shops: isolation, analysis and toxicological impact
title_full_unstemmed A comprehensive study of herbal blends from portuguese smart-shops: isolation, analysis and toxicological impact
title_sort A comprehensive study of herbal blends from portuguese smart-shops: isolation, analysis and toxicological impact
author Lopes, Inês Costa
author_facet Lopes, Inês Costa
author_role author
dc.contributor.none.fl_str_mv Quintas, Alexandre
Soeiro, Rita
Repositório Comum
dc.contributor.author.fl_str_mv Lopes, Inês Costa
dc.subject.por.fl_str_mv Synthetic cannabinoids
JWH-018
JWH-122
JWH-210
HPLC-UV
LC-MS/MS
GC-MS
Smoking machine
Toxicity
topic Synthetic cannabinoids
JWH-018
JWH-122
JWH-210
HPLC-UV
LC-MS/MS
GC-MS
Smoking machine
Toxicity
description Dissertação de mestrado Erasmus Mundus para obtenção de grau de mestre em Técnicas Laboratoriais Forenses
publishDate 2014
dc.date.none.fl_str_mv 2014-09-18T11:31:20Z
2014-07-01T00:00:00Z
2014-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.26/6714
201541505
url http://hdl.handle.net/10400.26/6714
identifier_str_mv 201541505
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Instituto Superior de Ciências da Saúde Egas Moniz
publisher.none.fl_str_mv Instituto Superior de Ciências da Saúde Egas Moniz
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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