Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/151617 |
Resumo: | This research was funded by FCT—Fundação para a Ciência e a Tecnologia, I.P. within the project “Yeast4FoodMed” (POCI-01-0145-FEDER-030936) through national funds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement. This work was also financed by national funds from FCT, in the scope of the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. Elisabete Coelho (CDL-CTTRI-88-ARH/2018—REF. 049-88-ARH/2018) and Benedita Pinheiro thank the research contracts funded by FCT through program DL 57/2016—Norma transitória. |
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Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carrierscarbohydrate microarraysDC-SIGNDectin-1Dectin-2delivery systemsglucansin vitro digestioninnate immune systemmannoproteinsThis research was funded by FCT—Fundação para a Ciência e a Tecnologia, I.P. within the project “Yeast4FoodMed” (POCI-01-0145-FEDER-030936) through national funds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement. This work was also financed by national funds from FCT, in the scope of the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. Elisabete Coelho (CDL-CTTRI-88-ARH/2018—REF. 049-88-ARH/2018) and Benedita Pinheiro thank the research contracts funded by FCT through program DL 57/2016—Norma transitória.Brewer’s spent yeast (BSY) microcapsules have a complex network of cell-wall polysaccharides that are induced by brewing when compared to the baker’s yeast (Saccharomyces cerevisiae) microcapsules. These are rich in (β1→3)-glucans and covalently linked to (α1→4)- and (β1→4)-glucans in addition to residual mannoproteins. S. cerevisiae is often used as a drug delivery system due to its immunostimulatory potential conferred by the presence of (β1→3)-glucans. Similarly, BSY microcapsules could also be used in the encapsulation of compounds or drug delivery systems with the advantage of resisting digestion conferred by (β1→4)-glucans and promoting a broader immunomodulatory response. This work aims to study the feasibility of BSY microcapsules that are the result of alkali and subcritical water extraction processes, as oral carriers for food and biomedical applications by (1) evaluating the resistance of BSY microcapsules to in vitro digestion (IVD), (2) their recognition by the human Dectin-1 immune receptor after IVD, and (3) the recognition of IVD-solubilized material by different mammalian immune receptors. IVD digested 44–63% of the material, depending on the extraction process. The non-digested material, despite some visible agglutination and deformation of the microcapsules, preserved their spherical shape and was enriched in (β1→3)-glucans. These microcapsules were all recognized by the human Dectin-1 immune receptor. The digested material was differentially recognized by a variety of lectins of the immune system related to (β1→3)-glucans, glycogen, and mannans. These results show the potential of BSY microcapsules to be used as oral carriers for food and biomedical applications.UCIBIO - Applied Molecular Biosciences UnitDQ - Departamento de QuímicaRUNReis, Sofia F.Martins, Vítor J.Bastos, RitaLima, TâniaCorreia, Viviana G.Pinheiro, Benedita A.Silva, Lisete M.Palma, Angelina S.Ferreira, PaulaVilanova, ManuelCoimbra, Manuel A.Coelho, Elisabete2023-04-05T22:15:50Z2023-01-052023-01-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article15application/pdfhttp://hdl.handle.net/10362/151617engReis, S. F., Martins, V. J., Bastos, R., Lima, T., Correia, V. G., Pinheiro, B. A., Silva, L. M., Palma, A. S., Ferreira, P., Vilanova, M., Coimbra, M. A., & Coelho, E. (2023). Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers. Foods, 12(2), [246]. https://doi.org/10.3390/foods120202462304-8158PURE: 52165178https://doi.org/10.3390/foods12020246info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:34:03Zoai:run.unl.pt:10362/151617Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:54:39.598837Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers |
| title |
Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers |
| spellingShingle |
Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers Reis, Sofia F. carbohydrate microarrays DC-SIGN Dectin-1 Dectin-2 delivery systems glucans in vitro digestion innate immune system mannoproteins |
| title_short |
Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers |
| title_full |
Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers |
| title_fullStr |
Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers |
| title_full_unstemmed |
Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers |
| title_sort |
Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers |
| author |
Reis, Sofia F. |
| author_facet |
Reis, Sofia F. Martins, Vítor J. Bastos, Rita Lima, Tânia Correia, Viviana G. Pinheiro, Benedita A. Silva, Lisete M. Palma, Angelina S. Ferreira, Paula Vilanova, Manuel Coimbra, Manuel A. Coelho, Elisabete |
| author_role |
author |
| author2 |
Martins, Vítor J. Bastos, Rita Lima, Tânia Correia, Viviana G. Pinheiro, Benedita A. Silva, Lisete M. Palma, Angelina S. Ferreira, Paula Vilanova, Manuel Coimbra, Manuel A. Coelho, Elisabete |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
UCIBIO - Applied Molecular Biosciences Unit DQ - Departamento de Química RUN |
| dc.contributor.author.fl_str_mv |
Reis, Sofia F. Martins, Vítor J. Bastos, Rita Lima, Tânia Correia, Viviana G. Pinheiro, Benedita A. Silva, Lisete M. Palma, Angelina S. Ferreira, Paula Vilanova, Manuel Coimbra, Manuel A. Coelho, Elisabete |
| dc.subject.por.fl_str_mv |
carbohydrate microarrays DC-SIGN Dectin-1 Dectin-2 delivery systems glucans in vitro digestion innate immune system mannoproteins |
| topic |
carbohydrate microarrays DC-SIGN Dectin-1 Dectin-2 delivery systems glucans in vitro digestion innate immune system mannoproteins |
| description |
This research was funded by FCT—Fundação para a Ciência e a Tecnologia, I.P. within the project “Yeast4FoodMed” (POCI-01-0145-FEDER-030936) through national funds and, where applicable, co-financed by the FEDER, within the PT2020 Partnership Agreement. This work was also financed by national funds from FCT, in the scope of the project LA/P/0140/2020 of the Associate Laboratory Institute for Health and Bioeconomy—i4HB. Elisabete Coelho (CDL-CTTRI-88-ARH/2018—REF. 049-88-ARH/2018) and Benedita Pinheiro thank the research contracts funded by FCT through program DL 57/2016—Norma transitória. |
| publishDate |
2023 |
| dc.date.none.fl_str_mv |
2023-04-05T22:15:50Z 2023-01-05 2023-01-05T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/151617 |
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http://hdl.handle.net/10362/151617 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
Reis, S. F., Martins, V. J., Bastos, R., Lima, T., Correia, V. G., Pinheiro, B. A., Silva, L. M., Palma, A. S., Ferreira, P., Vilanova, M., Coimbra, M. A., & Coelho, E. (2023). Feasibility of Brewer’s Spent Yeast Microcapsules as Targeted Oral Carriers. Foods, 12(2), [246]. https://doi.org/10.3390/foods12020246 2304-8158 PURE: 52165178 https://doi.org/10.3390/foods12020246 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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15 application/pdf |
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