MicroRNA profiling in human breast cancer cell lines exposed to the anti-neoplastic drug cediranib
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/45040 |
Resumo: | Cediranib, a pan-tyrosine kinase inhibitor is showing promising results for the treatment of several solid tumours. In breast cancer, its effects remain unclear, and there are no predictive biomarkers. Several studies have examined the expression profiles of microRNAs (miRNAs) in response to different chemotherapy treatments and found that the expression patterns may be associated with the treatment response. Therefore, our aim was to evaluate the cellular behaviour and differential expression profiles of miRNAs in breast cancer cell lines exposed to cediranib. The biological effect of this drug was measured by viability, migration, invasion and cell death in in vitro assays. Signaling pathways were assessed using a human phospho-receptor tyrosine kinase array. Furthermore, using a miRNA array and quantitative real-time PCR (qRT-PCR), we assessed the relative expression of miRNAs following cediranib treatment. The breast cancer cell lines exhibited a distinct cytotoxic response to cediranib treatment. Cediranib exposure resulted in a decrease in the cell migration and invasion of all the breast cancer cell lines. Treatment with cediranib appeared to be able to modulate the activation of several RTKs that are targets of cediranib such as EGFR and a new potential target ROR2. Furthermore, this drug was able to modulate the expression profile of different microRNAs such as miR-494, miR-923, miR-449a, miR-449b and miR-886-3 in breast cancer cell lines. These miRNAs are reported to regulate genes involved in important molecular processes, according to bioinformatics prediction tools. |
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MicroRNA profiling in human breast cancer cell lines exposed to the anti-neoplastic drug cediranibBreast cancer cell linesXediranibCellular behaviourMicroRNA expressionMicroarrayscediranibScience & TechnologyCediranib, a pan-tyrosine kinase inhibitor is showing promising results for the treatment of several solid tumours. In breast cancer, its effects remain unclear, and there are no predictive biomarkers. Several studies have examined the expression profiles of microRNAs (miRNAs) in response to different chemotherapy treatments and found that the expression patterns may be associated with the treatment response. Therefore, our aim was to evaluate the cellular behaviour and differential expression profiles of miRNAs in breast cancer cell lines exposed to cediranib. The biological effect of this drug was measured by viability, migration, invasion and cell death in in vitro assays. Signaling pathways were assessed using a human phospho-receptor tyrosine kinase array. Furthermore, using a miRNA array and quantitative real-time PCR (qRT-PCR), we assessed the relative expression of miRNAs following cediranib treatment. The breast cancer cell lines exhibited a distinct cytotoxic response to cediranib treatment. Cediranib exposure resulted in a decrease in the cell migration and invasion of all the breast cancer cell lines. Treatment with cediranib appeared to be able to modulate the activation of several RTKs that are targets of cediranib such as EGFR and a new potential target ROR2. Furthermore, this drug was able to modulate the expression profile of different microRNAs such as miR-494, miR-923, miR-449a, miR-449b and miR-886-3 in breast cancer cell lines. These miRNAs are reported to regulate genes involved in important molecular processes, according to bioinformatics prediction tools.We would like to thank Olga Martinho and Celine Pinheiro for assisting in the cellular experiments. This study received financial support from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP Proc. no. 2010/16796-0, São Paulo, brazil).Spandidos PublicationsUniversidade do MinhoBordinhão, A. L. R.Evangelista, Adriane F.Oliveira, R. J. S.Macedo, TacianeSilveira, Henrique C. S.Reis, R. M.Marques, Marcia M. C.20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/45040engBordinhao, A. L. R., Evangelist, A. F., Oliveira, R. J. S., MacEdo, T., Silveir, H. C., Rei, R. M., & Marques, M. M. (2016). MicroRNA profiling in human breast cancer cell lines exposed to the anti-neoplastic drug cediranib. Oncology Reports, 36(6), 3197-3206. doi: 10.3892/or.2016.51531021-335X1791-243110.3892/or.2016.515327748845https://www.spandidos-publications.com/or/36/6/3197info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:52:29Zoai:repositorium.sdum.uminho.pt:1822/45040Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:51:37.851634Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
MicroRNA profiling in human breast cancer cell lines exposed to the anti-neoplastic drug cediranib |
title |
MicroRNA profiling in human breast cancer cell lines exposed to the anti-neoplastic drug cediranib |
spellingShingle |
MicroRNA profiling in human breast cancer cell lines exposed to the anti-neoplastic drug cediranib Bordinhão, A. L. R. Breast cancer cell lines Xediranib Cellular behaviour MicroRNA expression Microarrays cediranib Science & Technology |
title_short |
MicroRNA profiling in human breast cancer cell lines exposed to the anti-neoplastic drug cediranib |
title_full |
MicroRNA profiling in human breast cancer cell lines exposed to the anti-neoplastic drug cediranib |
title_fullStr |
MicroRNA profiling in human breast cancer cell lines exposed to the anti-neoplastic drug cediranib |
title_full_unstemmed |
MicroRNA profiling in human breast cancer cell lines exposed to the anti-neoplastic drug cediranib |
title_sort |
MicroRNA profiling in human breast cancer cell lines exposed to the anti-neoplastic drug cediranib |
author |
Bordinhão, A. L. R. |
author_facet |
Bordinhão, A. L. R. Evangelista, Adriane F. Oliveira, R. J. S. Macedo, Taciane Silveira, Henrique C. S. Reis, R. M. Marques, Marcia M. C. |
author_role |
author |
author2 |
Evangelista, Adriane F. Oliveira, R. J. S. Macedo, Taciane Silveira, Henrique C. S. Reis, R. M. Marques, Marcia M. C. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Bordinhão, A. L. R. Evangelista, Adriane F. Oliveira, R. J. S. Macedo, Taciane Silveira, Henrique C. S. Reis, R. M. Marques, Marcia M. C. |
dc.subject.por.fl_str_mv |
Breast cancer cell lines Xediranib Cellular behaviour MicroRNA expression Microarrays cediranib Science & Technology |
topic |
Breast cancer cell lines Xediranib Cellular behaviour MicroRNA expression Microarrays cediranib Science & Technology |
description |
Cediranib, a pan-tyrosine kinase inhibitor is showing promising results for the treatment of several solid tumours. In breast cancer, its effects remain unclear, and there are no predictive biomarkers. Several studies have examined the expression profiles of microRNAs (miRNAs) in response to different chemotherapy treatments and found that the expression patterns may be associated with the treatment response. Therefore, our aim was to evaluate the cellular behaviour and differential expression profiles of miRNAs in breast cancer cell lines exposed to cediranib. The biological effect of this drug was measured by viability, migration, invasion and cell death in in vitro assays. Signaling pathways were assessed using a human phospho-receptor tyrosine kinase array. Furthermore, using a miRNA array and quantitative real-time PCR (qRT-PCR), we assessed the relative expression of miRNAs following cediranib treatment. The breast cancer cell lines exhibited a distinct cytotoxic response to cediranib treatment. Cediranib exposure resulted in a decrease in the cell migration and invasion of all the breast cancer cell lines. Treatment with cediranib appeared to be able to modulate the activation of several RTKs that are targets of cediranib such as EGFR and a new potential target ROR2. Furthermore, this drug was able to modulate the expression profile of different microRNAs such as miR-494, miR-923, miR-449a, miR-449b and miR-886-3 in breast cancer cell lines. These miRNAs are reported to regulate genes involved in important molecular processes, according to bioinformatics prediction tools. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2016-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/45040 |
url |
http://hdl.handle.net/1822/45040 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bordinhao, A. L. R., Evangelist, A. F., Oliveira, R. J. S., MacEdo, T., Silveir, H. C., Rei, R. M., & Marques, M. M. (2016). MicroRNA profiling in human breast cancer cell lines exposed to the anti-neoplastic drug cediranib. Oncology Reports, 36(6), 3197-3206. doi: 10.3892/or.2016.5153 1021-335X 1791-2431 10.3892/or.2016.5153 27748845 https://www.spandidos-publications.com/or/36/6/3197 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Spandidos Publications |
publisher.none.fl_str_mv |
Spandidos Publications |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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