Fixed-bed column process as a strategy for separation and purification of Cephamycin C from fermented broth
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/34727 |
Resumo: | Fixed-bed column processes using the anionic resin Q Sepharose XL were evaluated for Cephamycin C (CepC) purification from fermentation broth. Breakthrough and desorption curves were obtained for different flow rates (2.5, 5.0 and 7.5 mL/min). The elution method consisted of a stepwise gradient using NaCl solutions (0.1, 0.3 and 0.5%), which resulted in the separation of CepC from others antibiotics. The flow rate did not interfere on adsorption during loading of the column, but band broadening was observed during elution as the flow rate increased. After the ion exchange process, the fractions containing CepC were submitted to solid phase extraction using a C18 cartridge to remove salts. Analyses of the broth used to feed the column and of the salt-free fractions by LC-MS showed a reduction in the concentration of some contaminants (possibly penicillin N, deacetylcephalosporin C, and deacetoxycephalosporin C) compared to the concentration of CepC. In conclusion, ion exchange process followed by adsorption on a C18 adsorbent was demonstrated to be a selective and efficient procedure to purify CepC from fermentation broth. |
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Fixed-bed column process as a strategy for separation and purification of Cephamycin C from fermented brothScience & TechnologyFixed-bed column processes using the anionic resin Q Sepharose XL were evaluated for Cephamycin C (CepC) purification from fermentation broth. Breakthrough and desorption curves were obtained for different flow rates (2.5, 5.0 and 7.5 mL/min). The elution method consisted of a stepwise gradient using NaCl solutions (0.1, 0.3 and 0.5%), which resulted in the separation of CepC from others antibiotics. The flow rate did not interfere on adsorption during loading of the column, but band broadening was observed during elution as the flow rate increased. After the ion exchange process, the fractions containing CepC were submitted to solid phase extraction using a C18 cartridge to remove salts. Analyses of the broth used to feed the column and of the salt-free fractions by LC-MS showed a reduction in the concentration of some contaminants (possibly penicillin N, deacetylcephalosporin C, and deacetoxycephalosporin C) compared to the concentration of CepC. In conclusion, ion exchange process followed by adsorption on a C18 adsorbent was demonstrated to be a selective and efficient procedure to purify CepC from fermentation broth.The authors acknowledge CAPES, CNPq, and FAPESP for the financial support.American Chemical SocietyUniversidade do MinhoOliveira, Liliane M.Brites, Luciana M.Bustamante, MaritzaParpot, PierTeixeira, J. A.Mussatto, Solange I.Barboza, Marlei2015-03-032015-03-03T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/34727engOliveira, Liliane M.; Brites, Luciana M.; Bustamante, Maritza C; Parpot, Pier; Teixeira, J. A.; Mussatto, Solange I.; Barboza, Marlei, Fixed-bed column process as a strategy for separation and purification of Cephamycin C from fermented broth. Industrial and Engineering Chemistry Research, 54(11), 3018-3026, 20150888-58851520-504510.1021/ie504499zhttp://pubs.acs.org/journal/iecredinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:48:38Zoai:repositorium.sdum.uminho.pt:1822/34727Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:46:55.742157Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Fixed-bed column process as a strategy for separation and purification of Cephamycin C from fermented broth |
title |
Fixed-bed column process as a strategy for separation and purification of Cephamycin C from fermented broth |
spellingShingle |
Fixed-bed column process as a strategy for separation and purification of Cephamycin C from fermented broth Oliveira, Liliane M. Science & Technology |
title_short |
Fixed-bed column process as a strategy for separation and purification of Cephamycin C from fermented broth |
title_full |
Fixed-bed column process as a strategy for separation and purification of Cephamycin C from fermented broth |
title_fullStr |
Fixed-bed column process as a strategy for separation and purification of Cephamycin C from fermented broth |
title_full_unstemmed |
Fixed-bed column process as a strategy for separation and purification of Cephamycin C from fermented broth |
title_sort |
Fixed-bed column process as a strategy for separation and purification of Cephamycin C from fermented broth |
author |
Oliveira, Liliane M. |
author_facet |
Oliveira, Liliane M. Brites, Luciana M. Bustamante, Maritza Parpot, Pier Teixeira, J. A. Mussatto, Solange I. Barboza, Marlei |
author_role |
author |
author2 |
Brites, Luciana M. Bustamante, Maritza Parpot, Pier Teixeira, J. A. Mussatto, Solange I. Barboza, Marlei |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Oliveira, Liliane M. Brites, Luciana M. Bustamante, Maritza Parpot, Pier Teixeira, J. A. Mussatto, Solange I. Barboza, Marlei |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Fixed-bed column processes using the anionic resin Q Sepharose XL were evaluated for Cephamycin C (CepC) purification from fermentation broth. Breakthrough and desorption curves were obtained for different flow rates (2.5, 5.0 and 7.5 mL/min). The elution method consisted of a stepwise gradient using NaCl solutions (0.1, 0.3 and 0.5%), which resulted in the separation of CepC from others antibiotics. The flow rate did not interfere on adsorption during loading of the column, but band broadening was observed during elution as the flow rate increased. After the ion exchange process, the fractions containing CepC were submitted to solid phase extraction using a C18 cartridge to remove salts. Analyses of the broth used to feed the column and of the salt-free fractions by LC-MS showed a reduction in the concentration of some contaminants (possibly penicillin N, deacetylcephalosporin C, and deacetoxycephalosporin C) compared to the concentration of CepC. In conclusion, ion exchange process followed by adsorption on a C18 adsorbent was demonstrated to be a selective and efficient procedure to purify CepC from fermentation broth. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-03 2015-03-03T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/34727 |
url |
http://hdl.handle.net/1822/34727 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oliveira, Liliane M.; Brites, Luciana M.; Bustamante, Maritza C; Parpot, Pier; Teixeira, J. A.; Mussatto, Solange I.; Barboza, Marlei, Fixed-bed column process as a strategy for separation and purification of Cephamycin C from fermented broth. Industrial and Engineering Chemistry Research, 54(11), 3018-3026, 2015 0888-5885 1520-5045 10.1021/ie504499z http://pubs.acs.org/journal/iecred |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133040931241984 |