Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal column
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | https://hdl.handle.net/1822/19928 |
Resumo: | In this study, a simple and efficient process to purify fructo-oligosaccharides (FOS) from a fermentative broth was proposed using a single activated charcoal column. The FOS adsorption onto the activated charcoal was modeled by a pseudo-second order model. Several volumes and concentrations of water/ ethanol were studied to optimize the selective desorption of sugars from the broth mixture at 25 8C. Mixtures containing 50.6% (w/w) of FOS (FOS content in the fermentative broth) were purified to 92.9% (w/w) with a FOS recovery of 74.5% (w/w). Moreover, with the proposed process, fractions with purity up to 97% (w/w) of FOS were obtained. This purification process was also found to be efficient in the desalting of the fermentative broth. |
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Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal columnFructooligosaccharidesPrebioticsRecoveryActivated charcoal columnScience & TechnologyIn this study, a simple and efficient process to purify fructo-oligosaccharides (FOS) from a fermentative broth was proposed using a single activated charcoal column. The FOS adsorption onto the activated charcoal was modeled by a pseudo-second order model. Several volumes and concentrations of water/ ethanol were studied to optimize the selective desorption of sugars from the broth mixture at 25 8C. Mixtures containing 50.6% (w/w) of FOS (FOS content in the fermentative broth) were purified to 92.9% (w/w) with a FOS recovery of 74.5% (w/w). Moreover, with the proposed process, fractions with purity up to 97% (w/w) of FOS were obtained. This purification process was also found to be efficient in the desalting of the fermentative broth.The first author gratefully acknowledges to Fundacao para a Ciencia e a Tecnologia (Portugal) for the PhD Grant received (reference SFRH/BD/32514/2006).ElsevierUniversidade do MinhoNobre, ClarisseTeixeira, J. A.Rodrigues, L. R.20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/19928eng1871-678410.1016/j.nbt.2011.11.00622100432http://www.sciencedirect.com/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:49:03Zoai:repositorium.sdum.uminho.pt:1822/19928Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:47:26.763455Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal column |
title |
Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal column |
spellingShingle |
Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal column Nobre, Clarisse Fructooligosaccharides Prebiotics Recovery Activated charcoal column Science & Technology |
title_short |
Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal column |
title_full |
Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal column |
title_fullStr |
Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal column |
title_full_unstemmed |
Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal column |
title_sort |
Fructo-oligosaccharides purification from a fermentative broth using an activated charcoal column |
author |
Nobre, Clarisse |
author_facet |
Nobre, Clarisse Teixeira, J. A. Rodrigues, L. R. |
author_role |
author |
author2 |
Teixeira, J. A. Rodrigues, L. R. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Nobre, Clarisse Teixeira, J. A. Rodrigues, L. R. |
dc.subject.por.fl_str_mv |
Fructooligosaccharides Prebiotics Recovery Activated charcoal column Science & Technology |
topic |
Fructooligosaccharides Prebiotics Recovery Activated charcoal column Science & Technology |
description |
In this study, a simple and efficient process to purify fructo-oligosaccharides (FOS) from a fermentative broth was proposed using a single activated charcoal column. The FOS adsorption onto the activated charcoal was modeled by a pseudo-second order model. Several volumes and concentrations of water/ ethanol were studied to optimize the selective desorption of sugars from the broth mixture at 25 8C. Mixtures containing 50.6% (w/w) of FOS (FOS content in the fermentative broth) were purified to 92.9% (w/w) with a FOS recovery of 74.5% (w/w). Moreover, with the proposed process, fractions with purity up to 97% (w/w) of FOS were obtained. This purification process was also found to be efficient in the desalting of the fermentative broth. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012 2012-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://hdl.handle.net/1822/19928 |
url |
https://hdl.handle.net/1822/19928 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1871-6784 10.1016/j.nbt.2011.11.006 22100432 http://www.sciencedirect.com/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133048171659264 |