Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/6841 |
Resumo: | At this time the European Union regulations require that the heterotrophic plate counts (HPC) of mineral waters be assessed at two recovery temperatures: 22°C for 72 h and 37°C for 24 h. This procedure is time consuming and expensive. Development of new rapid methods for microbiological assessment of the microbial flora in the bottled water is an industry-driven need. The objectives of this work were to develop a method for the HPC that utilises only one recovery temperature and one incubation period and evaluate the use of, the LIVE/DEAD® BacLight™ Bacterial Viability Kit, 5-cyano-2,3-ditotyl tetrazolium chloride (CTC) and impedance methods to enumerate viable bacteria in bottled mineral water. Results showed that incubation at 30°C could be used instead of incubation at 22°C and 37°C. Good correlation exists between counts at 30°C and counts at 22°C (r>0.90) and all the pathogens important in mineral water analyses grow similarly at 30°C and 37°C during 24 h. It was demonstrated that impedance methods might be useful to the mineral water industry as a rapid indicator of microbiological quality of the water. Results obtained with BacLight and CTC were similar to those obtained with plate counts. |
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Improved methods for the enumeration of heterotrophic bacteria in bottled mineral watersBacLightCTCHeterotrophic plate countsImpedance methodsMineral waterAt this time the European Union regulations require that the heterotrophic plate counts (HPC) of mineral waters be assessed at two recovery temperatures: 22°C for 72 h and 37°C for 24 h. This procedure is time consuming and expensive. Development of new rapid methods for microbiological assessment of the microbial flora in the bottled water is an industry-driven need. The objectives of this work were to develop a method for the HPC that utilises only one recovery temperature and one incubation period and evaluate the use of, the LIVE/DEAD® BacLight™ Bacterial Viability Kit, 5-cyano-2,3-ditotyl tetrazolium chloride (CTC) and impedance methods to enumerate viable bacteria in bottled mineral water. Results showed that incubation at 30°C could be used instead of incubation at 22°C and 37°C. Good correlation exists between counts at 30°C and counts at 22°C (r>0.90) and all the pathogens important in mineral water analyses grow similarly at 30°C and 37°C during 24 h. It was demonstrated that impedance methods might be useful to the mineral water industry as a rapid indicator of microbiological quality of the water. Results obtained with BacLight and CTC were similar to those obtained with plate counts.ElsevierVeritati - Repositório Institucional da Universidade Católica PortuguesaRamalho, RitaCunha, JoaquimTeixeira, PaulaGibbs, Paul A.2011-10-22T16:25:24Z20012001-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/6841engRAMALHO, Rita ...[et al.] - Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters. Journal of Microbiological Methods. ISSN 0167-7012. Vol. 44, n.º2 (2001), p. 97–103info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:10:37Zoai:repositorio.ucp.pt:10400.14/6841Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:05:51.441434Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters |
title |
Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters |
spellingShingle |
Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters Ramalho, Rita BacLight CTC Heterotrophic plate counts Impedance methods Mineral water |
title_short |
Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters |
title_full |
Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters |
title_fullStr |
Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters |
title_full_unstemmed |
Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters |
title_sort |
Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters |
author |
Ramalho, Rita |
author_facet |
Ramalho, Rita Cunha, Joaquim Teixeira, Paula Gibbs, Paul A. |
author_role |
author |
author2 |
Cunha, Joaquim Teixeira, Paula Gibbs, Paul A. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Ramalho, Rita Cunha, Joaquim Teixeira, Paula Gibbs, Paul A. |
dc.subject.por.fl_str_mv |
BacLight CTC Heterotrophic plate counts Impedance methods Mineral water |
topic |
BacLight CTC Heterotrophic plate counts Impedance methods Mineral water |
description |
At this time the European Union regulations require that the heterotrophic plate counts (HPC) of mineral waters be assessed at two recovery temperatures: 22°C for 72 h and 37°C for 24 h. This procedure is time consuming and expensive. Development of new rapid methods for microbiological assessment of the microbial flora in the bottled water is an industry-driven need. The objectives of this work were to develop a method for the HPC that utilises only one recovery temperature and one incubation period and evaluate the use of, the LIVE/DEAD® BacLight™ Bacterial Viability Kit, 5-cyano-2,3-ditotyl tetrazolium chloride (CTC) and impedance methods to enumerate viable bacteria in bottled mineral water. Results showed that incubation at 30°C could be used instead of incubation at 22°C and 37°C. Good correlation exists between counts at 30°C and counts at 22°C (r>0.90) and all the pathogens important in mineral water analyses grow similarly at 30°C and 37°C during 24 h. It was demonstrated that impedance methods might be useful to the mineral water industry as a rapid indicator of microbiological quality of the water. Results obtained with BacLight and CTC were similar to those obtained with plate counts. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001 2001-01-01T00:00:00Z 2011-10-22T16:25:24Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/6841 |
url |
http://hdl.handle.net/10400.14/6841 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
RAMALHO, Rita ...[et al.] - Improved methods for the enumeration of heterotrophic bacteria in bottled mineral waters. Journal of Microbiological Methods. ISSN 0167-7012. Vol. 44, n.º2 (2001), p. 97–103 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799131724429393920 |