An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus

Detalhes bibliográficos
Autor(a) principal: Bento-Silva, Andreia
Data de Publicação: 2019
Outros Autores: Gonçalves, Letice, Mecha, Elsa, Pereira, Filipe, Patto, Maria Carlota Vaz, Bronze, Maria Do Rosário
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/107253
Resumo: This research was funded by EUROPEAN UNION'S SEVENTH FRAMEWORK PROGRAMME for research, technological development and demonstration under grant agreement number 613551 and by FUNDACAO PARA A CIENCIA E TECNOLOGIA (IF/01337/2014, PTDC/AGR-TEC/0992/2014) and PORTUGAL 2020 to the Portuguese Mass Spectrometry Network, grant number LISBOA-01-0145-FEDER-402-022125.
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spelling An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativusGrass peaHILIC columnHPLC-MS/MSLathyrus sativusα-ODAPβ-ODAPAnalytical ChemistryChemistry (miscellaneous)Molecular MedicinePharmaceutical ScienceDrug DiscoveryPhysical and Theoretical ChemistryOrganic ChemistryThis research was funded by EUROPEAN UNION'S SEVENTH FRAMEWORK PROGRAMME for research, technological development and demonstration under grant agreement number 613551 and by FUNDACAO PARA A CIENCIA E TECNOLOGIA (IF/01337/2014, PTDC/AGR-TEC/0992/2014) and PORTUGAL 2020 to the Portuguese Mass Spectrometry Network, grant number LISBOA-01-0145-FEDER-402-022125.β-N-Oxalyl-l-α,β-diaminopropionic acid (β-ODAP) is a non-protein amino acid present in Lathyrus sativus (grass pea) and other Lathyrus species, in parallel with its nontoxic isomer, α-ODAP. When consuming grass pea for several months as staple food, β-ODAP may cause neurolathyrism, a motor neuron degeneration syndrome. Therefore, the independent quantification of both ODAP isomers instead of only the total amount in grass pea allows the identification of less toxic varieties and the development of tools to support breeding for improving grass pea quality. In this work, a simple and fast HPLC-MS/MS method was developed without sample derivatization, using a hydrophilic interaction chromatography (HILIC) column and an isocratic gradient of eluents for 18 min, which allowed the determination of both α- and β-ODAP. The proposed method was fully validated and applied to the determination of α- and β-ODAP contents in a diverse collection of 107 grass pea accessions representative of the main grass pea-growing geographical regions in the world, with the prompt identification of contrasting accessions. β-ODAP content in the analyzed grass pea samples ranged from 0.45 ± 0.02 to 6.04 ± 0.45 mg g-1. The moderate correlation found between α- and β-ODAP contents (0.65) in this collection reinforces the importance of the independent quantification of both ODAP isomers.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)Faculdade de Ciências e Tecnologia (FCT)RUNBento-Silva, AndreiaGonçalves, LeticeMecha, ElsaPereira, FilipePatto, Maria Carlota VazBronze, Maria Do Rosário2020-11-16T23:58:20Z2019-08-222019-08-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/107253engPURE: 18899587https://doi.org/10.3390/molecules24173043info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:51:56Zoai:run.unl.pt:10362/107253Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:40:53.963752Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus
title An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus
spellingShingle An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus
Bento-Silva, Andreia
Grass pea
HILIC column
HPLC-MS/MS
Lathyrus sativus
α-ODAP
β-ODAP
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
title_short An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus
title_full An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus
title_fullStr An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus
title_full_unstemmed An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus
title_sort An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus
author Bento-Silva, Andreia
author_facet Bento-Silva, Andreia
Gonçalves, Letice
Mecha, Elsa
Pereira, Filipe
Patto, Maria Carlota Vaz
Bronze, Maria Do Rosário
author_role author
author2 Gonçalves, Letice
Mecha, Elsa
Pereira, Filipe
Patto, Maria Carlota Vaz
Bronze, Maria Do Rosário
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
Faculdade de Ciências e Tecnologia (FCT)
RUN
dc.contributor.author.fl_str_mv Bento-Silva, Andreia
Gonçalves, Letice
Mecha, Elsa
Pereira, Filipe
Patto, Maria Carlota Vaz
Bronze, Maria Do Rosário
dc.subject.por.fl_str_mv Grass pea
HILIC column
HPLC-MS/MS
Lathyrus sativus
α-ODAP
β-ODAP
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
topic Grass pea
HILIC column
HPLC-MS/MS
Lathyrus sativus
α-ODAP
β-ODAP
Analytical Chemistry
Chemistry (miscellaneous)
Molecular Medicine
Pharmaceutical Science
Drug Discovery
Physical and Theoretical Chemistry
Organic Chemistry
description This research was funded by EUROPEAN UNION'S SEVENTH FRAMEWORK PROGRAMME for research, technological development and demonstration under grant agreement number 613551 and by FUNDACAO PARA A CIENCIA E TECNOLOGIA (IF/01337/2014, PTDC/AGR-TEC/0992/2014) and PORTUGAL 2020 to the Portuguese Mass Spectrometry Network, grant number LISBOA-01-0145-FEDER-402-022125.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-22
2019-08-22T00:00:00Z
2020-11-16T23:58:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/107253
url http://hdl.handle.net/10362/107253
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv PURE: 18899587
https://doi.org/10.3390/molecules24173043
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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