An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/107253 |
Resumo: | This research was funded by EUROPEAN UNION'S SEVENTH FRAMEWORK PROGRAMME for research, technological development and demonstration under grant agreement number 613551 and by FUNDACAO PARA A CIENCIA E TECNOLOGIA (IF/01337/2014, PTDC/AGR-TEC/0992/2014) and PORTUGAL 2020 to the Portuguese Mass Spectrometry Network, grant number LISBOA-01-0145-FEDER-402-022125. |
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An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativusGrass peaHILIC columnHPLC-MS/MSLathyrus sativusα-ODAPβ-ODAPAnalytical ChemistryChemistry (miscellaneous)Molecular MedicinePharmaceutical ScienceDrug DiscoveryPhysical and Theoretical ChemistryOrganic ChemistryThis research was funded by EUROPEAN UNION'S SEVENTH FRAMEWORK PROGRAMME for research, technological development and demonstration under grant agreement number 613551 and by FUNDACAO PARA A CIENCIA E TECNOLOGIA (IF/01337/2014, PTDC/AGR-TEC/0992/2014) and PORTUGAL 2020 to the Portuguese Mass Spectrometry Network, grant number LISBOA-01-0145-FEDER-402-022125.β-N-Oxalyl-l-α,β-diaminopropionic acid (β-ODAP) is a non-protein amino acid present in Lathyrus sativus (grass pea) and other Lathyrus species, in parallel with its nontoxic isomer, α-ODAP. When consuming grass pea for several months as staple food, β-ODAP may cause neurolathyrism, a motor neuron degeneration syndrome. Therefore, the independent quantification of both ODAP isomers instead of only the total amount in grass pea allows the identification of less toxic varieties and the development of tools to support breeding for improving grass pea quality. In this work, a simple and fast HPLC-MS/MS method was developed without sample derivatization, using a hydrophilic interaction chromatography (HILIC) column and an isocratic gradient of eluents for 18 min, which allowed the determination of both α- and β-ODAP. The proposed method was fully validated and applied to the determination of α- and β-ODAP contents in a diverse collection of 107 grass pea accessions representative of the main grass pea-growing geographical regions in the world, with the prompt identification of contrasting accessions. β-ODAP content in the analyzed grass pea samples ranged from 0.45 ± 0.02 to 6.04 ± 0.45 mg g-1. The moderate correlation found between α- and β-ODAP contents (0.65) in this collection reinforces the importance of the independent quantification of both ODAP isomers.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)Faculdade de Ciências e Tecnologia (FCT)RUNBento-Silva, AndreiaGonçalves, LeticeMecha, ElsaPereira, FilipePatto, Maria Carlota VazBronze, Maria Do Rosário2020-11-16T23:58:20Z2019-08-222019-08-22T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/107253engPURE: 18899587https://doi.org/10.3390/molecules24173043info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:51:56Zoai:run.unl.pt:10362/107253Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:40:53.963752Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus |
title |
An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus |
spellingShingle |
An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus Bento-Silva, Andreia Grass pea HILIC column HPLC-MS/MS Lathyrus sativus α-ODAP β-ODAP Analytical Chemistry Chemistry (miscellaneous) Molecular Medicine Pharmaceutical Science Drug Discovery Physical and Theoretical Chemistry Organic Chemistry |
title_short |
An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus |
title_full |
An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus |
title_fullStr |
An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus |
title_full_unstemmed |
An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus |
title_sort |
An improved HILIC HPLC-MS/MS method for the determination of β-ODAP and Its α isomer in lathyrus sativus |
author |
Bento-Silva, Andreia |
author_facet |
Bento-Silva, Andreia Gonçalves, Letice Mecha, Elsa Pereira, Filipe Patto, Maria Carlota Vaz Bronze, Maria Do Rosário |
author_role |
author |
author2 |
Gonçalves, Letice Mecha, Elsa Pereira, Filipe Patto, Maria Carlota Vaz Bronze, Maria Do Rosário |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Instituto de Tecnologia Química e Biológica António Xavier (ITQB) Faculdade de Ciências e Tecnologia (FCT) RUN |
dc.contributor.author.fl_str_mv |
Bento-Silva, Andreia Gonçalves, Letice Mecha, Elsa Pereira, Filipe Patto, Maria Carlota Vaz Bronze, Maria Do Rosário |
dc.subject.por.fl_str_mv |
Grass pea HILIC column HPLC-MS/MS Lathyrus sativus α-ODAP β-ODAP Analytical Chemistry Chemistry (miscellaneous) Molecular Medicine Pharmaceutical Science Drug Discovery Physical and Theoretical Chemistry Organic Chemistry |
topic |
Grass pea HILIC column HPLC-MS/MS Lathyrus sativus α-ODAP β-ODAP Analytical Chemistry Chemistry (miscellaneous) Molecular Medicine Pharmaceutical Science Drug Discovery Physical and Theoretical Chemistry Organic Chemistry |
description |
This research was funded by EUROPEAN UNION'S SEVENTH FRAMEWORK PROGRAMME for research, technological development and demonstration under grant agreement number 613551 and by FUNDACAO PARA A CIENCIA E TECNOLOGIA (IF/01337/2014, PTDC/AGR-TEC/0992/2014) and PORTUGAL 2020 to the Portuguese Mass Spectrometry Network, grant number LISBOA-01-0145-FEDER-402-022125. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-22 2019-08-22T00:00:00Z 2020-11-16T23:58:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/107253 |
url |
http://hdl.handle.net/10362/107253 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PURE: 18899587 https://doi.org/10.3390/molecules24173043 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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