Step-by-step design of proteins for small molecule interaction: a review on recent milestones

Detalhes bibliográficos
Autor(a) principal: Pereira, José M.
Data de Publicação: 2021
Outros Autores: Vieira, Maria, Santos, Sérgio M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/31316
Resumo: Protein design is the field of synthetic biology that aims at developing de-novo custom made proteins and peptides for specific applications. Despite exploring an ambitious goal, recent computational advances in both hardware and software technologies have paved the way to high-throughput screening and detailed design of novel folds and improved functionalities. Modern advances in the field of protein design for small molecule targeting are described in this review, organized in a step-by-step fashion: from the conception of a new or upgraded active binding site, to scaffold design, sequence optimization and experimental expression of the custom protein. In each step, contemporary examples are described, and state-of-the art software is briefly explored.
id RCAP_2e9c43b3bc45ce116ed610089d8fba70
oai_identifier_str oai:ria.ua.pt:10773/31316
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Step-by-step design of proteins for small molecule interaction: a review on recent milestonesProtein designComputational chemistryDe-novo designProtein synthesisProtein design is the field of synthetic biology that aims at developing de-novo custom made proteins and peptides for specific applications. Despite exploring an ambitious goal, recent computational advances in both hardware and software technologies have paved the way to high-throughput screening and detailed design of novel folds and improved functionalities. Modern advances in the field of protein design for small molecule targeting are described in this review, organized in a step-by-step fashion: from the conception of a new or upgraded active binding site, to scaffold design, sequence optimization and experimental expression of the custom protein. In each step, contemporary examples are described, and state-of-the art software is briefly explored.Wiley2022-05-02T00:00:00Z2021-05-02T00:00:00Z2021-05-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/31316eng0961-836810.1002/pro.4098Pereira, José M.Vieira, MariaSantos, Sérgio M.info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:00:26Zoai:ria.ua.pt:10773/31316Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:03:13.564241Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Step-by-step design of proteins for small molecule interaction: a review on recent milestones
title Step-by-step design of proteins for small molecule interaction: a review on recent milestones
spellingShingle Step-by-step design of proteins for small molecule interaction: a review on recent milestones
Pereira, José M.
Protein design
Computational chemistry
De-novo design
Protein synthesis
title_short Step-by-step design of proteins for small molecule interaction: a review on recent milestones
title_full Step-by-step design of proteins for small molecule interaction: a review on recent milestones
title_fullStr Step-by-step design of proteins for small molecule interaction: a review on recent milestones
title_full_unstemmed Step-by-step design of proteins for small molecule interaction: a review on recent milestones
title_sort Step-by-step design of proteins for small molecule interaction: a review on recent milestones
author Pereira, José M.
author_facet Pereira, José M.
Vieira, Maria
Santos, Sérgio M.
author_role author
author2 Vieira, Maria
Santos, Sérgio M.
author2_role author
author
dc.contributor.author.fl_str_mv Pereira, José M.
Vieira, Maria
Santos, Sérgio M.
dc.subject.por.fl_str_mv Protein design
Computational chemistry
De-novo design
Protein synthesis
topic Protein design
Computational chemistry
De-novo design
Protein synthesis
description Protein design is the field of synthetic biology that aims at developing de-novo custom made proteins and peptides for specific applications. Despite exploring an ambitious goal, recent computational advances in both hardware and software technologies have paved the way to high-throughput screening and detailed design of novel folds and improved functionalities. Modern advances in the field of protein design for small molecule targeting are described in this review, organized in a step-by-step fashion: from the conception of a new or upgraded active binding site, to scaffold design, sequence optimization and experimental expression of the custom protein. In each step, contemporary examples are described, and state-of-the art software is briefly explored.
publishDate 2021
dc.date.none.fl_str_mv 2021-05-02T00:00:00Z
2021-05-02
2022-05-02T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/31316
url http://hdl.handle.net/10773/31316
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0961-8368
10.1002/pro.4098
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799137687650697216

Registros relacionados