Step-by-step design of proteins for small molecule interaction: a review on recent milestones
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10773/31316 |
Resumo: | Protein design is the field of synthetic biology that aims at developing de-novo custom made proteins and peptides for specific applications. Despite exploring an ambitious goal, recent computational advances in both hardware and software technologies have paved the way to high-throughput screening and detailed design of novel folds and improved functionalities. Modern advances in the field of protein design for small molecule targeting are described in this review, organized in a step-by-step fashion: from the conception of a new or upgraded active binding site, to scaffold design, sequence optimization and experimental expression of the custom protein. In each step, contemporary examples are described, and state-of-the art software is briefly explored. |
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Step-by-step design of proteins for small molecule interaction: a review on recent milestonesProtein designComputational chemistryDe-novo designProtein synthesisProtein design is the field of synthetic biology that aims at developing de-novo custom made proteins and peptides for specific applications. Despite exploring an ambitious goal, recent computational advances in both hardware and software technologies have paved the way to high-throughput screening and detailed design of novel folds and improved functionalities. Modern advances in the field of protein design for small molecule targeting are described in this review, organized in a step-by-step fashion: from the conception of a new or upgraded active binding site, to scaffold design, sequence optimization and experimental expression of the custom protein. In each step, contemporary examples are described, and state-of-the art software is briefly explored.Wiley2022-05-02T00:00:00Z2021-05-02T00:00:00Z2021-05-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/31316eng0961-836810.1002/pro.4098Pereira, José M.Vieira, MariaSantos, Sérgio M.info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:00:26Zoai:ria.ua.pt:10773/31316Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:03:13.564241Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Step-by-step design of proteins for small molecule interaction: a review on recent milestones |
title |
Step-by-step design of proteins for small molecule interaction: a review on recent milestones |
spellingShingle |
Step-by-step design of proteins for small molecule interaction: a review on recent milestones Pereira, José M. Protein design Computational chemistry De-novo design Protein synthesis |
title_short |
Step-by-step design of proteins for small molecule interaction: a review on recent milestones |
title_full |
Step-by-step design of proteins for small molecule interaction: a review on recent milestones |
title_fullStr |
Step-by-step design of proteins for small molecule interaction: a review on recent milestones |
title_full_unstemmed |
Step-by-step design of proteins for small molecule interaction: a review on recent milestones |
title_sort |
Step-by-step design of proteins for small molecule interaction: a review on recent milestones |
author |
Pereira, José M. |
author_facet |
Pereira, José M. Vieira, Maria Santos, Sérgio M. |
author_role |
author |
author2 |
Vieira, Maria Santos, Sérgio M. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Pereira, José M. Vieira, Maria Santos, Sérgio M. |
dc.subject.por.fl_str_mv |
Protein design Computational chemistry De-novo design Protein synthesis |
topic |
Protein design Computational chemistry De-novo design Protein synthesis |
description |
Protein design is the field of synthetic biology that aims at developing de-novo custom made proteins and peptides for specific applications. Despite exploring an ambitious goal, recent computational advances in both hardware and software technologies have paved the way to high-throughput screening and detailed design of novel folds and improved functionalities. Modern advances in the field of protein design for small molecule targeting are described in this review, organized in a step-by-step fashion: from the conception of a new or upgraded active binding site, to scaffold design, sequence optimization and experimental expression of the custom protein. In each step, contemporary examples are described, and state-of-the art software is briefly explored. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-05-02T00:00:00Z 2021-05-02 2022-05-02T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10773/31316 |
url |
http://hdl.handle.net/10773/31316 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0961-8368 10.1002/pro.4098 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
eu_rights_str_mv |
embargoedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799137687650697216 |