Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.3/6247 |
Resumo: | During Camellia sinensis tea processing, manufacturers usually remove the internodes, which are classified as waste. This study presents the first determination of plant part contribution, particularly internodes, to green tea quality, in order to find the best blend to maximize impact on human health. Catechins, caffeine and free amino acid (FAA) profiles were determined by RP-HPLC/DAD, total phenolics (TPC) and total flavonoids (TFC) by FolinCiocalteu and colorimetric methodologies, respectively, and antioxidant activities by free radical-scavenging activity (FRSA), ferric-reducing antioxidant power (FRAP) and ferrous ion-chelating (FIC) methods. Individual esterified catechins content decreased as follows: epicatechin-3-gallate > epigallocatechin-3-gallate ≫ gallocatecin-3-gallate, and epicatechin derivatives content ranged from 63.91 to 91.22% of total catechins. Caffeine content was higher in internodes. L-theanine, histidine, asparagine, phenylalanine, glutamic acid and methionine were the major FAAs, and internodes contained the highest amounts of L-theanine and histidine (17 and 13.73 mg/g of sample, respectively). TPC ranged from 201.51 to 265.48 mg gallic acid equivalents/g dry extract (DE) and TFC ranged from 23.84 to 72.02 mg rutin equivalents/g DE. Internodes presented the lowest FRSA (EC50 ¼ 6.10–13.50 μg/mL), FRAP (EC50 ¼ 5.70–11.40 μg/mL) and FIC activity (36.96–79.21%). Bud presented the highest FRSA and FRAP, and budþ1stþ2ndleaves þ internodes the highest FIC activity. The results revealed the potential contribution of the internodes to green tea quality and, consequently, to human health. |
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Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensisCatechin ProfilesFree Amino Acid ProfilesInternode Waste ValorizationL-Theanine/Caffeine ContentRp-Hplc AnalysisTea AntioxidantsTotal FlavonoidsTotal PhenolicsDuring Camellia sinensis tea processing, manufacturers usually remove the internodes, which are classified as waste. This study presents the first determination of plant part contribution, particularly internodes, to green tea quality, in order to find the best blend to maximize impact on human health. Catechins, caffeine and free amino acid (FAA) profiles were determined by RP-HPLC/DAD, total phenolics (TPC) and total flavonoids (TFC) by FolinCiocalteu and colorimetric methodologies, respectively, and antioxidant activities by free radical-scavenging activity (FRSA), ferric-reducing antioxidant power (FRAP) and ferrous ion-chelating (FIC) methods. Individual esterified catechins content decreased as follows: epicatechin-3-gallate > epigallocatechin-3-gallate ≫ gallocatecin-3-gallate, and epicatechin derivatives content ranged from 63.91 to 91.22% of total catechins. Caffeine content was higher in internodes. L-theanine, histidine, asparagine, phenylalanine, glutamic acid and methionine were the major FAAs, and internodes contained the highest amounts of L-theanine and histidine (17 and 13.73 mg/g of sample, respectively). TPC ranged from 201.51 to 265.48 mg gallic acid equivalents/g dry extract (DE) and TFC ranged from 23.84 to 72.02 mg rutin equivalents/g DE. Internodes presented the lowest FRSA (EC50 ¼ 6.10–13.50 μg/mL), FRAP (EC50 ¼ 5.70–11.40 μg/mL) and FIC activity (36.96–79.21%). Bud presented the highest FRSA and FRAP, and budþ1stþ2ndleaves þ internodes the highest FIC activity. The results revealed the potential contribution of the internodes to green tea quality and, consequently, to human health.This study was supported by PO Açores 2020 and by the Community Structural Funds FEDER and FSE for the 2014-2020 programming period, with implementation in the Azores Region, under the project "ACORES-01-0247-FEDER-000014".Elsevier BVRepositório da Universidade dos AçoresPaiva, Lisete S.Lima, ElisabeteMotta, MadalenaMarcone, MassimoBaptista, José2022-03-18T16:57:03Z2020-112022-01-28T18:56:55Z2020-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.3/6247engPaiva, L., Lima, E., Motta, M., Marcone, M., & Baptista, J. (2020). Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis. “CURRENT RESEARCH IN FOOD SCIENCE”, 3, 227–234. https://doi.org/10.1016/j.crfs.2020.07.0042665-9271cv-prod-268725810.1016/j.crfs.2020.07.004PMC778293033426532000658380400026info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-12-20T14:34:35Zoai:repositorio.uac.pt:10400.3/6247Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:28:19.480183Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis |
title |
Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis |
spellingShingle |
Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis Paiva, Lisete S. Catechin Profiles Free Amino Acid Profiles Internode Waste Valorization L-Theanine/Caffeine Content Rp-Hplc Analysis Tea Antioxidants Total Flavonoids Total Phenolics |
title_short |
Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis |
title_full |
Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis |
title_fullStr |
Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis |
title_full_unstemmed |
Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis |
title_sort |
Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis |
author |
Paiva, Lisete S. |
author_facet |
Paiva, Lisete S. Lima, Elisabete Motta, Madalena Marcone, Massimo Baptista, José |
author_role |
author |
author2 |
Lima, Elisabete Motta, Madalena Marcone, Massimo Baptista, José |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade dos Açores |
dc.contributor.author.fl_str_mv |
Paiva, Lisete S. Lima, Elisabete Motta, Madalena Marcone, Massimo Baptista, José |
dc.subject.por.fl_str_mv |
Catechin Profiles Free Amino Acid Profiles Internode Waste Valorization L-Theanine/Caffeine Content Rp-Hplc Analysis Tea Antioxidants Total Flavonoids Total Phenolics |
topic |
Catechin Profiles Free Amino Acid Profiles Internode Waste Valorization L-Theanine/Caffeine Content Rp-Hplc Analysis Tea Antioxidants Total Flavonoids Total Phenolics |
description |
During Camellia sinensis tea processing, manufacturers usually remove the internodes, which are classified as waste. This study presents the first determination of plant part contribution, particularly internodes, to green tea quality, in order to find the best blend to maximize impact on human health. Catechins, caffeine and free amino acid (FAA) profiles were determined by RP-HPLC/DAD, total phenolics (TPC) and total flavonoids (TFC) by FolinCiocalteu and colorimetric methodologies, respectively, and antioxidant activities by free radical-scavenging activity (FRSA), ferric-reducing antioxidant power (FRAP) and ferrous ion-chelating (FIC) methods. Individual esterified catechins content decreased as follows: epicatechin-3-gallate > epigallocatechin-3-gallate ≫ gallocatecin-3-gallate, and epicatechin derivatives content ranged from 63.91 to 91.22% of total catechins. Caffeine content was higher in internodes. L-theanine, histidine, asparagine, phenylalanine, glutamic acid and methionine were the major FAAs, and internodes contained the highest amounts of L-theanine and histidine (17 and 13.73 mg/g of sample, respectively). TPC ranged from 201.51 to 265.48 mg gallic acid equivalents/g dry extract (DE) and TFC ranged from 23.84 to 72.02 mg rutin equivalents/g DE. Internodes presented the lowest FRSA (EC50 ¼ 6.10–13.50 μg/mL), FRAP (EC50 ¼ 5.70–11.40 μg/mL) and FIC activity (36.96–79.21%). Bud presented the highest FRSA and FRAP, and budþ1stþ2ndleaves þ internodes the highest FIC activity. The results revealed the potential contribution of the internodes to green tea quality and, consequently, to human health. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-11 2020-11-01T00:00:00Z 2022-03-18T16:57:03Z 2022-01-28T18:56:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.3/6247 |
url |
http://hdl.handle.net/10400.3/6247 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Paiva, L., Lima, E., Motta, M., Marcone, M., & Baptista, J. (2020). Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis. “CURRENT RESEARCH IN FOOD SCIENCE”, 3, 227–234. https://doi.org/10.1016/j.crfs.2020.07.004 2665-9271 cv-prod-2687258 10.1016/j.crfs.2020.07.004 PMC7782930 33426532 000658380400026 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier BV |
publisher.none.fl_str_mv |
Elsevier BV |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130740899708928 |