Transcriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathways

Detalhes bibliográficos
Autor(a) principal: Enguita, Francisco J.
Data de Publicação: 2023
Outros Autores: Pereira, Sofia, Leitão, Ana Lúcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/155191
Resumo: Publisher Copyright: © 2023 by the authors. This research received no external funding
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spelling Transcriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathwaysacetaminophenbiodegradationfunctional networksPenicillium chrysogenumtranscriptomic analysisEcology, Evolution, Behavior and SystematicsPlant ScienceMicrobiology (medical)SDG 6 - Clean Water and SanitationSDG 14 - Life Below WaterPublisher Copyright: © 2023 by the authors. This research received no external funding(1) Background: Acetaminophen (APAP), an active component of many analgesic and antipyretic drugs, is one of the most concerning trace contaminants in the environment and is considered as an emergent pollutant of marine and aquatic ecosystems. Despite its biodegradability, APAP has become a recalcitrant compound due to the growth of the global population, the ease of availability, and the inefficient wastewater treatment applied. (2) Methods: In this study, we used a transcriptomic approach to obtain functional and metabolic insights about the metabolization of APAP by a phenol-degrading fungal strain, Penicillium chrysogenum var. halophenolicum. (3) Results: We determined that the transcriptomic profile exhibited by the fungal strain during APAP degradation was very dynamic, being characterized by an abundance of dysregulated transcripts which were proportional to the drug metabolization. Using a systems biology approach, we also inferred the protein functional interaction networks that could be related to APAP degradation. We proposed the involvement of intracellular and extracellular enzymes, such as amidases, cytochrome P450, laccases, and extradiol-dioxygenases, among others. (4) Conclusions: Our data suggested that the fungus could metabolize APAP via a complex metabolic pathway, generating nontoxic metabolites, which demonstrated its potential in the bioremediation of this drug.DQ - Departamento de QuímicaMEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de RecursosRUNEnguita, Francisco J.Pereira, SofiaLeitão, Ana Lúcia2023-07-12T22:20:23Z2023-03-272023-03-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article20application/pdfhttp://hdl.handle.net/10362/155191eng2309-608XPURE: 66105468https://doi.org/10.3390/jof9040408info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:37:43Zoai:run.unl.pt:10362/155191Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:55:58.005768Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Transcriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathways
title Transcriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathways
spellingShingle Transcriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathways
Enguita, Francisco J.
acetaminophen
biodegradation
functional networks
Penicillium chrysogenum
transcriptomic analysis
Ecology, Evolution, Behavior and Systematics
Plant Science
Microbiology (medical)
SDG 6 - Clean Water and Sanitation
SDG 14 - Life Below Water
title_short Transcriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathways
title_full Transcriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathways
title_fullStr Transcriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathways
title_full_unstemmed Transcriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathways
title_sort Transcriptomic Analysis of Acetaminophen Biodegradation by Penicillium chrysogenum var. halophenolicum and Insights into Energy and Stress Response Pathways
author Enguita, Francisco J.
author_facet Enguita, Francisco J.
Pereira, Sofia
Leitão, Ana Lúcia
author_role author
author2 Pereira, Sofia
Leitão, Ana Lúcia
author2_role author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
MEtRICS - Centro de Engenharia Mecânica e Sustentabilidade de Recursos
RUN
dc.contributor.author.fl_str_mv Enguita, Francisco J.
Pereira, Sofia
Leitão, Ana Lúcia
dc.subject.por.fl_str_mv acetaminophen
biodegradation
functional networks
Penicillium chrysogenum
transcriptomic analysis
Ecology, Evolution, Behavior and Systematics
Plant Science
Microbiology (medical)
SDG 6 - Clean Water and Sanitation
SDG 14 - Life Below Water
topic acetaminophen
biodegradation
functional networks
Penicillium chrysogenum
transcriptomic analysis
Ecology, Evolution, Behavior and Systematics
Plant Science
Microbiology (medical)
SDG 6 - Clean Water and Sanitation
SDG 14 - Life Below Water
description Publisher Copyright: © 2023 by the authors. This research received no external funding
publishDate 2023
dc.date.none.fl_str_mv 2023-07-12T22:20:23Z
2023-03-27
2023-03-27T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/155191
url http://hdl.handle.net/10362/155191
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2309-608X
PURE: 66105468
https://doi.org/10.3390/jof9040408
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 20
application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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