Stimuli-responsive chitosan-starch injectable hydrogels combined with encapsulated adipose-derived stromal cells for articular cartilage regeneration
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/20636 |
Resumo: | Tissue engineering strategies have been showing promising early results in articular cartilage lesions repair. Hydrogels based on natural origin polymers as chitosan glycerol-phosphate (CGP) thermosensitive formulation that can be implanted in a minimal invasive manner, represent a great promise as injectable scaffold choice for cartilage tissue engineering, but it lacks in mechanical properties. A different formulation, from which a firm texture gels results is, therefore, desirable. In this work we first aim to investigate the suitability of CGP to produce an injectable thermosensitive, pH-dependent solution, when combined with increasing concentrations of starch: 0.5% (I), 1% (II), and 1.5% (III). The data collected from the rheological measurements showed that the addition of starch to the CGP did not alter the transition temperature and confirmed the heating inducing gelation of all solutions, supporting the ability of these novel formulations to be applied as minimal invasive systems. The evaluation of the dynamic mechanical analysis of the hydrogels showed an increase in the storage modulus within increasing starch concentration, clearly demonstrating that best viscoelastic properties were obtained with the novel chitosan-starch based solution. The incorporation of starch also improved the degradation profile. All materials showed to be biocompatible through the cytotoxicity screening in vitro. These data suggested the potential of novel thermo-responsive chitosan-starch hydrogels to be used as injectable vehicles for cell delivery in cartilage tissue engineering applications. In a second phase, the potential of chitosan-b-glycerophosphate (CGP) and chitosan-bglycerophosphate- 1% starch (CST) hydrogels to induce chondrocytic differentiation and cartilage matrix accumulation were evaluated, as well as the influence of starch in the chondrogenesis of encapsulated adipose derived stromal (ADSC) cells. The ADSC were homogeneously encapsulated, remained viable, proliferated, and maintained the expression of typical chondrogenic markers genes, and deposited cartilage ECM molecules. Improved results were obtained within the novel CST constructs. The overall data suggest that chitosan-b-glycerophosphate-starch hydrogels could be considered for chondrogenic differentiation of adipose derived stromal cells for cartilage-engineered regeneration using minimal invasive techniques. |
id |
RCAP_2fb32ad2067deca95693a2daef27e86d |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/20636 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Stimuli-responsive chitosan-starch injectable hydrogels combined with encapsulated adipose-derived stromal cells for articular cartilage regenerationScience & TechnologyTissue engineering strategies have been showing promising early results in articular cartilage lesions repair. Hydrogels based on natural origin polymers as chitosan glycerol-phosphate (CGP) thermosensitive formulation that can be implanted in a minimal invasive manner, represent a great promise as injectable scaffold choice for cartilage tissue engineering, but it lacks in mechanical properties. A different formulation, from which a firm texture gels results is, therefore, desirable. In this work we first aim to investigate the suitability of CGP to produce an injectable thermosensitive, pH-dependent solution, when combined with increasing concentrations of starch: 0.5% (I), 1% (II), and 1.5% (III). The data collected from the rheological measurements showed that the addition of starch to the CGP did not alter the transition temperature and confirmed the heating inducing gelation of all solutions, supporting the ability of these novel formulations to be applied as minimal invasive systems. The evaluation of the dynamic mechanical analysis of the hydrogels showed an increase in the storage modulus within increasing starch concentration, clearly demonstrating that best viscoelastic properties were obtained with the novel chitosan-starch based solution. The incorporation of starch also improved the degradation profile. All materials showed to be biocompatible through the cytotoxicity screening in vitro. These data suggested the potential of novel thermo-responsive chitosan-starch hydrogels to be used as injectable vehicles for cell delivery in cartilage tissue engineering applications. In a second phase, the potential of chitosan-b-glycerophosphate (CGP) and chitosan-bglycerophosphate- 1% starch (CST) hydrogels to induce chondrocytic differentiation and cartilage matrix accumulation were evaluated, as well as the influence of starch in the chondrogenesis of encapsulated adipose derived stromal (ADSC) cells. The ADSC were homogeneously encapsulated, remained viable, proliferated, and maintained the expression of typical chondrogenic markers genes, and deposited cartilage ECM molecules. Improved results were obtained within the novel CST constructs. The overall data suggest that chitosan-b-glycerophosphate-starch hydrogels could be considered for chondrogenic differentiation of adipose derived stromal cells for cartilage-engineered regeneration using minimal invasive techniques.The authors acknowledge the financial support to the Portuguese Foundation for Science and Technology (FCT) for the PhD fellowship to H. Sa-Lima (SFRH/BD/21779/2005) and for the Project PTDC/QUI/68804/2006; the European Union funded STREP Project HIPPOCRATES (NM3-CT-2003-505758); and the European NoE EXPERTISSUES (NMP3-CT-2004-500283).Royal Society of ChemistryUniversidade do MinhoSá-Lima, HelenaCaridade, S. G.Mano, J. F.Reis, R. L.20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20636eng1744-683X10.1039/c0sm00041hhttp://pubs.rsc.org/en/content/articlelanding/2010/sm/c0sm00041hinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:08:07Zoai:repositorium.sdum.uminho.pt:1822/20636Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:59:18.600163Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Stimuli-responsive chitosan-starch injectable hydrogels combined with encapsulated adipose-derived stromal cells for articular cartilage regeneration |
title |
Stimuli-responsive chitosan-starch injectable hydrogels combined with encapsulated adipose-derived stromal cells for articular cartilage regeneration |
spellingShingle |
Stimuli-responsive chitosan-starch injectable hydrogels combined with encapsulated adipose-derived stromal cells for articular cartilage regeneration Sá-Lima, Helena Science & Technology |
title_short |
Stimuli-responsive chitosan-starch injectable hydrogels combined with encapsulated adipose-derived stromal cells for articular cartilage regeneration |
title_full |
Stimuli-responsive chitosan-starch injectable hydrogels combined with encapsulated adipose-derived stromal cells for articular cartilage regeneration |
title_fullStr |
Stimuli-responsive chitosan-starch injectable hydrogels combined with encapsulated adipose-derived stromal cells for articular cartilage regeneration |
title_full_unstemmed |
Stimuli-responsive chitosan-starch injectable hydrogels combined with encapsulated adipose-derived stromal cells for articular cartilage regeneration |
title_sort |
Stimuli-responsive chitosan-starch injectable hydrogels combined with encapsulated adipose-derived stromal cells for articular cartilage regeneration |
author |
Sá-Lima, Helena |
author_facet |
Sá-Lima, Helena Caridade, S. G. Mano, J. F. Reis, R. L. |
author_role |
author |
author2 |
Caridade, S. G. Mano, J. F. Reis, R. L. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Sá-Lima, Helena Caridade, S. G. Mano, J. F. Reis, R. L. |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
Tissue engineering strategies have been showing promising early results in articular cartilage lesions repair. Hydrogels based on natural origin polymers as chitosan glycerol-phosphate (CGP) thermosensitive formulation that can be implanted in a minimal invasive manner, represent a great promise as injectable scaffold choice for cartilage tissue engineering, but it lacks in mechanical properties. A different formulation, from which a firm texture gels results is, therefore, desirable. In this work we first aim to investigate the suitability of CGP to produce an injectable thermosensitive, pH-dependent solution, when combined with increasing concentrations of starch: 0.5% (I), 1% (II), and 1.5% (III). The data collected from the rheological measurements showed that the addition of starch to the CGP did not alter the transition temperature and confirmed the heating inducing gelation of all solutions, supporting the ability of these novel formulations to be applied as minimal invasive systems. The evaluation of the dynamic mechanical analysis of the hydrogels showed an increase in the storage modulus within increasing starch concentration, clearly demonstrating that best viscoelastic properties were obtained with the novel chitosan-starch based solution. The incorporation of starch also improved the degradation profile. All materials showed to be biocompatible through the cytotoxicity screening in vitro. These data suggested the potential of novel thermo-responsive chitosan-starch hydrogels to be used as injectable vehicles for cell delivery in cartilage tissue engineering applications. In a second phase, the potential of chitosan-b-glycerophosphate (CGP) and chitosan-bglycerophosphate- 1% starch (CST) hydrogels to induce chondrocytic differentiation and cartilage matrix accumulation were evaluated, as well as the influence of starch in the chondrogenesis of encapsulated adipose derived stromal (ADSC) cells. The ADSC were homogeneously encapsulated, remained viable, proliferated, and maintained the expression of typical chondrogenic markers genes, and deposited cartilage ECM molecules. Improved results were obtained within the novel CST constructs. The overall data suggest that chitosan-b-glycerophosphate-starch hydrogels could be considered for chondrogenic differentiation of adipose derived stromal cells for cartilage-engineered regeneration using minimal invasive techniques. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2010-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/20636 |
url |
http://hdl.handle.net/1822/20636 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1744-683X 10.1039/c0sm00041h http://pubs.rsc.org/en/content/articlelanding/2010/sm/c0sm00041h |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132384967262208 |