3D bioprinting of miniaturized tissues embedded in self-assembled nanoparticle-based fibrillar platforms
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/74752 |
Resumo: | The creation of microphysiological systems like tissue and organ-on-chip for in vitro modeling of human physiology and diseases is gathering increasing interest. However, the platforms used to build these systems have limitations concerning implementation, automation, and cost-effectiveness. Moreover, their typical plastic-based housing materials are poor recreations of native tissue extracellular matrix (ECM) and barriers. Here, the controlled self-assembly of plant-derived cellulose nanocrystals (CNC) is combined with the concept of 3D bioprinting in suspension baths for the direct biofabrication of microphysiological systems embedded within an ECM mimetic fibrillar support material. The developed support CNC fluid gel allows exceptionally high-resolution bioprinting of 3D constructs with arbitrary geometries and low restrictions of bioink choice. The further induction of CNC self-assembly with biocompatible calcium ions results in a transparent biomimetic nanoscaled fibrillar matrix that allows hosting different compartmentalized cell types and perfusable channels, has tailored permeability for biomacromolecules diffusion and cellular crosstalk, and holds structural stability to support long-term in vitro cell maturation. In summary, this xeno-free nanoscale CNC fibrillar matrix allows the biofabrication of hierarchical living constructs, opening new opportunities not only for developing physiologically relevant 3D in vitro models but also for a wide range of applications in regenerative medicine. |
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3D bioprinting of miniaturized tissues embedded in self-assembled nanoparticle-based fibrillar platformsBioprinting In vitro modelsCellulose nanocrystalsembedded bioprintingfibrillar matrixmicrophysiological systemsself-assemblyScience & TechnologyThe creation of microphysiological systems like tissue and organ-on-chip for in vitro modeling of human physiology and diseases is gathering increasing interest. However, the platforms used to build these systems have limitations concerning implementation, automation, and cost-effectiveness. Moreover, their typical plastic-based housing materials are poor recreations of native tissue extracellular matrix (ECM) and barriers. Here, the controlled self-assembly of plant-derived cellulose nanocrystals (CNC) is combined with the concept of 3D bioprinting in suspension baths for the direct biofabrication of microphysiological systems embedded within an ECM mimetic fibrillar support material. The developed support CNC fluid gel allows exceptionally high-resolution bioprinting of 3D constructs with arbitrary geometries and low restrictions of bioink choice. The further induction of CNC self-assembly with biocompatible calcium ions results in a transparent biomimetic nanoscaled fibrillar matrix that allows hosting different compartmentalized cell types and perfusable channels, has tailored permeability for biomacromolecules diffusion and cellular crosstalk, and holds structural stability to support long-term in vitro cell maturation. In summary, this xeno-free nanoscale CNC fibrillar matrix allows the biofabrication of hierarchical living constructs, opening new opportunities not only for developing physiologically relevant 3D in vitro models but also for a wide range of applications in regenerative medicine.The authors thank Hospital da Prelada (Porto, Portugal) for providing adipose tissue samples and Hospital Sao Joao (Porto, Portugal) for providing platelet concentrates. The authors acknowledge the financial support from project NORTE-01-0145-FEDER-000021 supported by Norte Portugal Regional Operational Program (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF); the European Union Framework Program for Research and Innovation HORIZON 2020, under the Twinning grant agreement no. 810850-Achilles, European Research Council grant agreement no. 772817, Fundacao para a Ciencia e a Tecnologia for the PhD grant for S.M.B PD/BD/129403/2017 financed through doctoral program in Tissue Engineering, Regenerative Medicine and Stem Cells (TERM&SC), and project PTDC/NAN-MAT/30595/2017. Schematics in Figures 1, 2, and 6 were created with BioRender.com. The authors thank Milan Sixt and Barbara B. Mendes for preliminary tests with CNC fluid gel. The authors thank David Caballero, Catarina Abreu, and Mandana Mombeinipour for providing endothelial cells and Virginia Brancato for breast cancer cells.WileyUniversidade do MinhoBakht, Syeda M.Gómez-Florit, ManuelLamers, Tara HelenaReis, R. L.Domingues, Rui M. A.Gomes, Manuela E.2021-082021-08-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/74752engBakht S. M., Gómez-Florit M., Lamers T., Reis R. L., Domingues R. M. A., Gomes M. E. 3D Bioprinting of Miniaturized Tissues Embedded in Self-Assembled Nanoparticle-Based Fibrillar Platforms, Advanced Functional Materials, Vol. 31, Issue 46, pp. 2104245-2104245, doi:10.1002/adfm.202104245, 20211616-301X1616-302810.1002/adfm.202104245https://onlinelibrary.wiley.com/doi/full/10.1002/adfm.202104245info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:58:57Zoai:repositorium.sdum.uminho.pt:1822/74752Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:48:43.707777Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
3D bioprinting of miniaturized tissues embedded in self-assembled nanoparticle-based fibrillar platforms |
title |
3D bioprinting of miniaturized tissues embedded in self-assembled nanoparticle-based fibrillar platforms |
spellingShingle |
3D bioprinting of miniaturized tissues embedded in self-assembled nanoparticle-based fibrillar platforms Bakht, Syeda M. Bioprinting In vitro models Cellulose nanocrystals embedded bioprinting fibrillar matrix microphysiological systems self-assembly Science & Technology |
title_short |
3D bioprinting of miniaturized tissues embedded in self-assembled nanoparticle-based fibrillar platforms |
title_full |
3D bioprinting of miniaturized tissues embedded in self-assembled nanoparticle-based fibrillar platforms |
title_fullStr |
3D bioprinting of miniaturized tissues embedded in self-assembled nanoparticle-based fibrillar platforms |
title_full_unstemmed |
3D bioprinting of miniaturized tissues embedded in self-assembled nanoparticle-based fibrillar platforms |
title_sort |
3D bioprinting of miniaturized tissues embedded in self-assembled nanoparticle-based fibrillar platforms |
author |
Bakht, Syeda M. |
author_facet |
Bakht, Syeda M. Gómez-Florit, Manuel Lamers, Tara Helena Reis, R. L. Domingues, Rui M. A. Gomes, Manuela E. |
author_role |
author |
author2 |
Gómez-Florit, Manuel Lamers, Tara Helena Reis, R. L. Domingues, Rui M. A. Gomes, Manuela E. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Bakht, Syeda M. Gómez-Florit, Manuel Lamers, Tara Helena Reis, R. L. Domingues, Rui M. A. Gomes, Manuela E. |
dc.subject.por.fl_str_mv |
Bioprinting In vitro models Cellulose nanocrystals embedded bioprinting fibrillar matrix microphysiological systems self-assembly Science & Technology |
topic |
Bioprinting In vitro models Cellulose nanocrystals embedded bioprinting fibrillar matrix microphysiological systems self-assembly Science & Technology |
description |
The creation of microphysiological systems like tissue and organ-on-chip for in vitro modeling of human physiology and diseases is gathering increasing interest. However, the platforms used to build these systems have limitations concerning implementation, automation, and cost-effectiveness. Moreover, their typical plastic-based housing materials are poor recreations of native tissue extracellular matrix (ECM) and barriers. Here, the controlled self-assembly of plant-derived cellulose nanocrystals (CNC) is combined with the concept of 3D bioprinting in suspension baths for the direct biofabrication of microphysiological systems embedded within an ECM mimetic fibrillar support material. The developed support CNC fluid gel allows exceptionally high-resolution bioprinting of 3D constructs with arbitrary geometries and low restrictions of bioink choice. The further induction of CNC self-assembly with biocompatible calcium ions results in a transparent biomimetic nanoscaled fibrillar matrix that allows hosting different compartmentalized cell types and perfusable channels, has tailored permeability for biomacromolecules diffusion and cellular crosstalk, and holds structural stability to support long-term in vitro cell maturation. In summary, this xeno-free nanoscale CNC fibrillar matrix allows the biofabrication of hierarchical living constructs, opening new opportunities not only for developing physiologically relevant 3D in vitro models but also for a wide range of applications in regenerative medicine. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-08 2021-08-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/74752 |
url |
http://hdl.handle.net/1822/74752 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bakht S. M., Gómez-Florit M., Lamers T., Reis R. L., Domingues R. M. A., Gomes M. E. 3D Bioprinting of Miniaturized Tissues Embedded in Self-Assembled Nanoparticle-Based Fibrillar Platforms, Advanced Functional Materials, Vol. 31, Issue 46, pp. 2104245-2104245, doi:10.1002/adfm.202104245, 2021 1616-301X 1616-3028 10.1002/adfm.202104245 https://onlinelibrary.wiley.com/doi/full/10.1002/adfm.202104245 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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