Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/148883 |
Resumo: | Funding Information: Funding: This research was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (n◦ 408369/2016-7), Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES), Programa Ciências do Mar (AUXPE-CIMAR-1956/2014), and Programa Nacional de Cooperação Acadêmica (CAPES/PROCAD). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
| id |
RCAP_31770368a0890f7661983159b107cdfa |
|---|---|
| oai_identifier_str |
oai:run.unl.pt:10362/148883 |
| network_acronym_str |
RCAP |
| network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository_id_str |
7160 |
| spelling |
Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activityanti-Trypanosoma cruzi activitybrown seaweedchagas diseasefucoidanRA0421 Public health. Hygiene. Preventive MedicineRM Therapeutics. PharmacologyQD ChemistryChemical Engineering(all)Materials Science(all)ParasitologySDG 3 - Good Health and Well-beingSDG 9 - Industry, Innovation, and InfrastructureSDG 7 - Affordable and Clean EnergySDG 11 - Sustainable Cities and CommunitiesFunding Information: Funding: This research was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (n◦ 408369/2016-7), Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES), Programa Ciências do Mar (AUXPE-CIMAR-1956/2014), and Programa Nacional de Cooperação Acadêmica (CAPES/PROCAD). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland.The brown seaweed Spatoglossum schröederi synthesizes three bioactive fucoidans, the most abundant of which is fucan A. This fucoidan was extracted and its identity was confirmed by chemical analysis, Fourier-transform infrared spectroscopy (FTIR), and agarose gel electrophoresis. Thereafter, silver nanoparticles containing fucan A (AgFuc) were produced using an environmentally friendly synthesis method. AgFuc synthesis was analyzed via UV–vis spectroscopy and FTIR, which confirmed the presence of both silver and fucan A in the AgFuc product. Dynamic light scattering (DLS), X-ray diffraction, scanning electron microscopy, and atomic force microscopy revealed that the AgFuc particles were ~180.0 nm in size and spherical in shape. DLS further demonstrated that AgFuc was stable for five months. Coupled plasma optical emission spectrometry showed that the AgFuc particles contained 5% silver and 95% sugar. AgFuc was shown to be more effective in inhibiting the ability of parasites to reduce MTT than fucan A or silver, regardless of treatment time. In addition, AgFuc induced the death of ~60% of parasites by necrosis and ~17% by apoptosis. Therefore, AgFuc induces damage to the parasites’ mitochondria, which suggests that it is an anti-Trypanosoma cruzi agent. This is the first study to analyze silver nanoparticles containing fucan as an anti-Trypanosoma cruzi agent. Our data indicate that AgFuc nanoparticles have potential therapeutic applications, which should be determined via preclinical in vitro and in vivo studies.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)Universidade NOVA de LisboaRUNSouza, Adriana OliveiraOliveira, Johny Wysllas de FreitasMoreno, Claudia Jéssica Gonsalvesde Medeiros, Mayra Jane CamposFernandes-Negreiros, Marília MedeirosSouza, Flavia Roberta MonteiroPontes, Daniel LimaSilva, Marcelo SousaRocha, Hugo Alexandre Oliveira2023-02-08T22:22:18Z2022-06-012022-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/148883eng2079-4991PURE: 50620770https://doi.org/10.3390/nano12122059info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:30:36Zoai:run.unl.pt:10362/148883Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:53:31.623252Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity |
| title |
Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity |
| spellingShingle |
Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity Souza, Adriana Oliveira anti-Trypanosoma cruzi activity brown seaweed chagas disease fucoidan RA0421 Public health. Hygiene. Preventive Medicine RM Therapeutics. Pharmacology QD Chemistry Chemical Engineering(all) Materials Science(all) Parasitology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 7 - Affordable and Clean Energy SDG 11 - Sustainable Cities and Communities |
| title_short |
Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity |
| title_full |
Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity |
| title_fullStr |
Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity |
| title_full_unstemmed |
Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity |
| title_sort |
Silver Nanoparticles Containing Fucoidan Synthesized by Green Method Have Anti-Trypanosoma cruzi Activity |
| author |
Souza, Adriana Oliveira |
| author_facet |
Souza, Adriana Oliveira Oliveira, Johny Wysllas de Freitas Moreno, Claudia Jéssica Gonsalves de Medeiros, Mayra Jane Campos Fernandes-Negreiros, Marília Medeiros Souza, Flavia Roberta Monteiro Pontes, Daniel Lima Silva, Marcelo Sousa Rocha, Hugo Alexandre Oliveira |
| author_role |
author |
| author2 |
Oliveira, Johny Wysllas de Freitas Moreno, Claudia Jéssica Gonsalves de Medeiros, Mayra Jane Campos Fernandes-Negreiros, Marília Medeiros Souza, Flavia Roberta Monteiro Pontes, Daniel Lima Silva, Marcelo Sousa Rocha, Hugo Alexandre Oliveira |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) Universidade NOVA de Lisboa RUN |
| dc.contributor.author.fl_str_mv |
Souza, Adriana Oliveira Oliveira, Johny Wysllas de Freitas Moreno, Claudia Jéssica Gonsalves de Medeiros, Mayra Jane Campos Fernandes-Negreiros, Marília Medeiros Souza, Flavia Roberta Monteiro Pontes, Daniel Lima Silva, Marcelo Sousa Rocha, Hugo Alexandre Oliveira |
| dc.subject.por.fl_str_mv |
anti-Trypanosoma cruzi activity brown seaweed chagas disease fucoidan RA0421 Public health. Hygiene. Preventive Medicine RM Therapeutics. Pharmacology QD Chemistry Chemical Engineering(all) Materials Science(all) Parasitology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 7 - Affordable and Clean Energy SDG 11 - Sustainable Cities and Communities |
| topic |
anti-Trypanosoma cruzi activity brown seaweed chagas disease fucoidan RA0421 Public health. Hygiene. Preventive Medicine RM Therapeutics. Pharmacology QD Chemistry Chemical Engineering(all) Materials Science(all) Parasitology SDG 3 - Good Health and Well-being SDG 9 - Industry, Innovation, and Infrastructure SDG 7 - Affordable and Clean Energy SDG 11 - Sustainable Cities and Communities |
| description |
Funding Information: Funding: This research was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico-CNPq (n◦ 408369/2016-7), Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES), Programa Ciências do Mar (AUXPE-CIMAR-1956/2014), and Programa Nacional de Cooperação Acadêmica (CAPES/PROCAD). Publisher Copyright: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-06-01 2022-06-01T00:00:00Z 2023-02-08T22:22:18Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/148883 |
| url |
http://hdl.handle.net/10362/148883 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2079-4991 PURE: 50620770 https://doi.org/10.3390/nano12122059 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
|
| _version_ |
1799138125955465216 |