Assessment of collagen content in fish skin: development of a flow analysis method for hydroxyproline determination
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/43138 |
Resumo: | This work describes the development of a flow injection method to determine hydroxyproline (HYP), one of collagen's most abundant amino acids. Collagen is a protein with several applications and high nutritional value. Evaluating the feasibility of using collagen from fish skin over its mammalian source is essential. The determination of HYP requires the pre-treatment and hydrolysis of the fish skin to break down collagen into its amino acids, and the HYP value quantified relates to the collagen content. The determination was based on the HYP oxidation with permanganate in an alkaline medium and the consequent decrease of colour intensity registered. Under optimal conditions, the developed method enables the determination of the HYP within the dynamic range of 23.8 to 500 mg L −1, with a limit of detection (LOD) of 2.6 mg L −1 and a limit of quantification (LOQ) of 23.8 mg L −1. Different samples were processed, and the digests were analysed by the proposed method and with the conventional procedure with good correlation (relative error < 7%). Moreover, the analyte quantification is performed faster, simpler, and more accurately, with less toxic solutions. The reproducibility of the developed method was also evaluated by calculating the relative standard deviation of the calibration curve slope (RSD < 1%). |
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Assessment of collagen content in fish skin: development of a flow analysis method for hydroxyproline determinationThis work describes the development of a flow injection method to determine hydroxyproline (HYP), one of collagen's most abundant amino acids. Collagen is a protein with several applications and high nutritional value. Evaluating the feasibility of using collagen from fish skin over its mammalian source is essential. The determination of HYP requires the pre-treatment and hydrolysis of the fish skin to break down collagen into its amino acids, and the HYP value quantified relates to the collagen content. The determination was based on the HYP oxidation with permanganate in an alkaline medium and the consequent decrease of colour intensity registered. Under optimal conditions, the developed method enables the determination of the HYP within the dynamic range of 23.8 to 500 mg L −1, with a limit of detection (LOD) of 2.6 mg L −1 and a limit of quantification (LOQ) of 23.8 mg L −1. Different samples were processed, and the digests were analysed by the proposed method and with the conventional procedure with good correlation (relative error < 7%). Moreover, the analyte quantification is performed faster, simpler, and more accurately, with less toxic solutions. The reproducibility of the developed method was also evaluated by calculating the relative standard deviation of the calibration curve slope (RSD < 1%).Veritati - Repositório Institucional da Universidade Católica PortuguesaMelo, Maria M. P.Mesquita, Raquel B. R.Coscueta, Ezequiel R.Pintado, Manuela E.Rangel, António O. S. S.2023-11-20T12:29:03Z2023-10-302023-10-30T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/43138eng1759-966010.1039/D3AY01589K8517563530337902049001099099200001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-05T01:38:00Zoai:repositorio.ucp.pt:10400.14/43138Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T22:54:10.347794Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Assessment of collagen content in fish skin: development of a flow analysis method for hydroxyproline determination |
title |
Assessment of collagen content in fish skin: development of a flow analysis method for hydroxyproline determination |
spellingShingle |
Assessment of collagen content in fish skin: development of a flow analysis method for hydroxyproline determination Melo, Maria M. P. |
title_short |
Assessment of collagen content in fish skin: development of a flow analysis method for hydroxyproline determination |
title_full |
Assessment of collagen content in fish skin: development of a flow analysis method for hydroxyproline determination |
title_fullStr |
Assessment of collagen content in fish skin: development of a flow analysis method for hydroxyproline determination |
title_full_unstemmed |
Assessment of collagen content in fish skin: development of a flow analysis method for hydroxyproline determination |
title_sort |
Assessment of collagen content in fish skin: development of a flow analysis method for hydroxyproline determination |
author |
Melo, Maria M. P. |
author_facet |
Melo, Maria M. P. Mesquita, Raquel B. R. Coscueta, Ezequiel R. Pintado, Manuela E. Rangel, António O. S. S. |
author_role |
author |
author2 |
Mesquita, Raquel B. R. Coscueta, Ezequiel R. Pintado, Manuela E. Rangel, António O. S. S. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Melo, Maria M. P. Mesquita, Raquel B. R. Coscueta, Ezequiel R. Pintado, Manuela E. Rangel, António O. S. S. |
description |
This work describes the development of a flow injection method to determine hydroxyproline (HYP), one of collagen's most abundant amino acids. Collagen is a protein with several applications and high nutritional value. Evaluating the feasibility of using collagen from fish skin over its mammalian source is essential. The determination of HYP requires the pre-treatment and hydrolysis of the fish skin to break down collagen into its amino acids, and the HYP value quantified relates to the collagen content. The determination was based on the HYP oxidation with permanganate in an alkaline medium and the consequent decrease of colour intensity registered. Under optimal conditions, the developed method enables the determination of the HYP within the dynamic range of 23.8 to 500 mg L −1, with a limit of detection (LOD) of 2.6 mg L −1 and a limit of quantification (LOQ) of 23.8 mg L −1. Different samples were processed, and the digests were analysed by the proposed method and with the conventional procedure with good correlation (relative error < 7%). Moreover, the analyte quantification is performed faster, simpler, and more accurately, with less toxic solutions. The reproducibility of the developed method was also evaluated by calculating the relative standard deviation of the calibration curve slope (RSD < 1%). |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-11-20T12:29:03Z 2023-10-30 2023-10-30T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/43138 |
url |
http://hdl.handle.net/10400.14/43138 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1759-9660 10.1039/D3AY01589K 85175635303 37902049 001099099200001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799135140060856320 |