Pruning wound protection products induce alterations in the wood mycobiome profile of Grapevines
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/27715 |
Resumo: | Fungal pathogens involved in grapevine trunk diseases (GTDs) may infect grapevines throughout their lifetime, from nursery to vineyard, via open wounds in stems, canes or roots. In vineyards, pruning wound protection products (PWPPs) offer the best means to reduce the chance of infection by GTD fungi. However, PWPPs may affect non-target microorganisms that comprise the natural endophytic mycobiome residing in treated canes, disrupting microbial homeostasis and indirectly influencing grapevine health. Using DNA metabarcoding, we characterized the endophytic mycobiome of one-year-old canes of cultivars Cabernet Sauvignon and Syrah in two vineyards in Portugal and Italy and assessed the impact of established and novel PWPPs on the fungal communities of treated canes. Our results reveal a large fungal diversity (176 taxa), and we report multiple genera never detected before in grapevine wood (e.g., Symmetrospora and Akenomyces). We found differences in mycobiome beta diversity when comparing vineyards (p = 0.01) but not cultivars (p > 0.05). When examining PWPP-treated canes, we detected cultivar- and vineyard-dependent alterations in both alpha and beta diversity. In addition, numerous fungal taxa were over- or under-represented when compared to control canes. Among them, Epicoccum sp., a beneficial genus with biological control potential, was negatively affected by selected PWPPs. This study demonstrates that PWPPs induce alterations in the fungal communities of grapevines, requiring an urgent evaluation of their direct and indirect effects on plants health with consideration of factors such as climatic conditions and yearly variations, in order to better advise viticulturists and policy makers. |
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Pruning wound protection products induce alterations in the wood mycobiome profile of GrapevinesVitis viniferamicrobiomegrapevine trunk diseasesCabernet SauvignonSyrahTrichodermacopperfungicidesbiological controlFungal pathogens involved in grapevine trunk diseases (GTDs) may infect grapevines throughout their lifetime, from nursery to vineyard, via open wounds in stems, canes or roots. In vineyards, pruning wound protection products (PWPPs) offer the best means to reduce the chance of infection by GTD fungi. However, PWPPs may affect non-target microorganisms that comprise the natural endophytic mycobiome residing in treated canes, disrupting microbial homeostasis and indirectly influencing grapevine health. Using DNA metabarcoding, we characterized the endophytic mycobiome of one-year-old canes of cultivars Cabernet Sauvignon and Syrah in two vineyards in Portugal and Italy and assessed the impact of established and novel PWPPs on the fungal communities of treated canes. Our results reveal a large fungal diversity (176 taxa), and we report multiple genera never detected before in grapevine wood (e.g., Symmetrospora and Akenomyces). We found differences in mycobiome beta diversity when comparing vineyards (p = 0.01) but not cultivars (p > 0.05). When examining PWPP-treated canes, we detected cultivar- and vineyard-dependent alterations in both alpha and beta diversity. In addition, numerous fungal taxa were over- or under-represented when compared to control canes. Among them, Epicoccum sp., a beneficial genus with biological control potential, was negatively affected by selected PWPPs. This study demonstrates that PWPPs induce alterations in the fungal communities of grapevines, requiring an urgent evaluation of their direct and indirect effects on plants health with consideration of factors such as climatic conditions and yearly variations, in order to better advise viticulturists and policy makers.MDPIRepositório da Universidade de LisboaDel Frari, GiovanniAggerbeck, Marie RønneGobbi, AlexIngrà, ChiaraVolpi, LorenzoNascimento, TeresaFerrandino, AlessandraHansen, Lars HestbjergFerreira, Ricardo Boavida2023-05-04T15:36:49Z20232023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/27715engDel Frari, G.; Aggerbeck, M.R.; Gobbi, A.; Ingrà, C.; Volpi, L.; Nascimento, T.; Ferrandino, A.; Hansen, L.H.; Ferreira, R.B. Pruning Wound Protection Products Induce Alterations in the Wood Mycobiome Profile of Grapevines. J. Fungi 2023, 9, 488.https://doi.org/10.3390/jof9040488info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-05-07T01:31:08Zoai:www.repository.utl.pt:10400.5/27715Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:50:58.673918Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Pruning wound protection products induce alterations in the wood mycobiome profile of Grapevines |
title |
Pruning wound protection products induce alterations in the wood mycobiome profile of Grapevines |
spellingShingle |
Pruning wound protection products induce alterations in the wood mycobiome profile of Grapevines Del Frari, Giovanni Vitis vinifera microbiome grapevine trunk diseases Cabernet Sauvignon Syrah Trichoderma copper fungicides biological control |
title_short |
Pruning wound protection products induce alterations in the wood mycobiome profile of Grapevines |
title_full |
Pruning wound protection products induce alterations in the wood mycobiome profile of Grapevines |
title_fullStr |
Pruning wound protection products induce alterations in the wood mycobiome profile of Grapevines |
title_full_unstemmed |
Pruning wound protection products induce alterations in the wood mycobiome profile of Grapevines |
title_sort |
Pruning wound protection products induce alterations in the wood mycobiome profile of Grapevines |
author |
Del Frari, Giovanni |
author_facet |
Del Frari, Giovanni Aggerbeck, Marie Rønne Gobbi, Alex Ingrà, Chiara Volpi, Lorenzo Nascimento, Teresa Ferrandino, Alessandra Hansen, Lars Hestbjerg Ferreira, Ricardo Boavida |
author_role |
author |
author2 |
Aggerbeck, Marie Rønne Gobbi, Alex Ingrà, Chiara Volpi, Lorenzo Nascimento, Teresa Ferrandino, Alessandra Hansen, Lars Hestbjerg Ferreira, Ricardo Boavida |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Del Frari, Giovanni Aggerbeck, Marie Rønne Gobbi, Alex Ingrà, Chiara Volpi, Lorenzo Nascimento, Teresa Ferrandino, Alessandra Hansen, Lars Hestbjerg Ferreira, Ricardo Boavida |
dc.subject.por.fl_str_mv |
Vitis vinifera microbiome grapevine trunk diseases Cabernet Sauvignon Syrah Trichoderma copper fungicides biological control |
topic |
Vitis vinifera microbiome grapevine trunk diseases Cabernet Sauvignon Syrah Trichoderma copper fungicides biological control |
description |
Fungal pathogens involved in grapevine trunk diseases (GTDs) may infect grapevines throughout their lifetime, from nursery to vineyard, via open wounds in stems, canes or roots. In vineyards, pruning wound protection products (PWPPs) offer the best means to reduce the chance of infection by GTD fungi. However, PWPPs may affect non-target microorganisms that comprise the natural endophytic mycobiome residing in treated canes, disrupting microbial homeostasis and indirectly influencing grapevine health. Using DNA metabarcoding, we characterized the endophytic mycobiome of one-year-old canes of cultivars Cabernet Sauvignon and Syrah in two vineyards in Portugal and Italy and assessed the impact of established and novel PWPPs on the fungal communities of treated canes. Our results reveal a large fungal diversity (176 taxa), and we report multiple genera never detected before in grapevine wood (e.g., Symmetrospora and Akenomyces). We found differences in mycobiome beta diversity when comparing vineyards (p = 0.01) but not cultivars (p > 0.05). When examining PWPP-treated canes, we detected cultivar- and vineyard-dependent alterations in both alpha and beta diversity. In addition, numerous fungal taxa were over- or under-represented when compared to control canes. Among them, Epicoccum sp., a beneficial genus with biological control potential, was negatively affected by selected PWPPs. This study demonstrates that PWPPs induce alterations in the fungal communities of grapevines, requiring an urgent evaluation of their direct and indirect effects on plants health with consideration of factors such as climatic conditions and yearly variations, in order to better advise viticulturists and policy makers. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-05-04T15:36:49Z 2023 2023-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/27715 |
url |
http://hdl.handle.net/10400.5/27715 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Del Frari, G.; Aggerbeck, M.R.; Gobbi, A.; Ingrà, C.; Volpi, L.; Nascimento, T.; Ferrandino, A.; Hansen, L.H.; Ferreira, R.B. Pruning Wound Protection Products Induce Alterations in the Wood Mycobiome Profile of Grapevines. J. Fungi 2023, 9, 488. https://doi.org/10.3390/jof9040488 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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